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EFEK SITOTOKSIK DAN ANTIPROLIFERATIF EKSTRAK KLOROFORM BUAH MAHKOTA DEWA TERHADAP SEL KANKER PAYUDARA T47D Nurulita, Nunuk Aries; Siswanto, Agus
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 3, No 4 (2007)
Publisher : Indonesian Research Gateway

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The study on anticancer activity of P. macrocarpa fruit extract had been done. This study was aimed to know cytotoxic and antiproliferative activity of P. macrocarpa Chloroform extract to T47D cell. Cytotoxic and antiproliferative assay of it was done by MTT method. The doubling time and AgNORs staining were used to identify proliferative inhibition of it. Apoptosis of T47D phenomenon wa identified by doubling time method with ethidium bromide-acridyne orange. The result showed than LC50 of P. macrocarpa fruit extract was 103,03 μg/ml. it has antiproliferative effect and prolong doubling time. It also induced apoptosis of T47D cell. ABSTRAK Telah dilakukan penelitian mengenai efek antikanker ekstrak buah mahkota dewa terhadap sel kanker payudara. Penelitian ini bertujuan untuk mengetahui efek sitotoksik, dan antiproliferasi ekstrak kloroform P. macrocarpa pada sel T47D. Uji sitotoksik dan antiproliferatif ekstrak kloroform buah mahkota dewa dilakukan dengan metode MTT. Untuk melihat penghambatan proliferasinya dilakukan dengan metode doubling time dan pengecatan AgNORs. Fenomena apoptosis sel T47D yang diberi perlakuan ekstrak kloroform P. macrocarpa dilihat dengan metode double stainning dengan etidium bromida-akridin oranye. Hasil penelitian menunjukkan nilai LC50 sebesar 103,03 μg/ml. ekstrak ini mempunyai efek antiproliferasi dan mampu memperpanjang waktu doubling time. Pengamatan apoptosis dengan double staining menggunakan etidium bromida-akridin oranye, menunjukkan dapat memacu apoptosis sel T47D.

OPTIMASI TABLET FLOATING TEOFILIN MENGGUNAKAN METODE DESAIN FAKTORIAL siswanto, agus; Hapsari, Indri; zulaikha, yusni ulfiani; rosmawati, eva
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol 3 No 1 (2016)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.05 KB)

ABSTRACTTheophylline is a drug used in the management of asthma. It has relatively short half-life and narrow therapeutic index with 5-20 μg/mL serum concentration. Thus, Floating tablet formulations that can increase the bioavailability of the drug. The aims of this study were to determine the effect of the excipient (HPMC K4M CR and NaHCO3) to the quality of tablets and determine the optimum formula of theophylline floating tablet. A 22 randomized factorial design was used in development of theophylline floating tablet with HPMC K4M CR (A) and NaHCO3 (B) as independent variables.The flowability of mass tablet, floating character, and dissolution profile were chosen as dependent variables.The results showed that NaHCO3 was the most important factor on increasing flowability of mass tablet. HPMC K4M CR was the dominant factor in lowering the Flag time, improving integrity of the tablet, and decreasing the release of drug. The composition of HPMC K4M CR 40.00 mg and NaHCO3 35.39 mg produced the optimum formula of theophylline floating tablet with desirability value of 0.978.

EFEK SITOTOKSIK DAN ANTIPROLIFERATIF EKSTRAK KLOROFORM BUAH MAHKOTA DEWA TERHADAP SEL KANKER PAYUDARA T47D Nurulita, Nunuk Aries; Siswanto, Agus
Jurnal Farmasi Indonesia Vol 3, No 4 (2007)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v3i4.87

The study on anticancer activity of P. macrocarpa fruit extract had been done. This study was aimed to know cytotoxic and antiproliferative activity of P. macrocarpa Chloroform extract to T47D cell. Cytotoxic and antiproliferative assay of it was done by MTT method. The doubling time and AgNORs staining were used to identify proliferative inhibition of it. Apoptosis of T47D phenomenon wa identified by doubling time method with ethidium bromide-acridyne orange. The result showed than LC50 of P. macrocarpa fruit extract was 103,03 Î¼g/ml. it has antiproliferative effect and prolong doubling time. It also induced apoptosis of T47D cell. Â ABSTRAK Telah dilakukan penelitian mengenai efek antikanker ekstrak buah mahkota dewa terhadap sel kanker payudara. Penelitian ini bertujuan untuk mengetahui efek sitotoksik, dan antiproliferasi ekstrak kloroform P. macrocarpa pada sel T47D. Â Uji sitotoksik dan antiproliferatif ekstrak kloroform buah mahkota dewa dilakukan dengan metode MTT. Untuk melihat penghambatan proliferasinya dilakukan dengan metode doubling time dan pengecatan AgNORs. Fenomena apoptosis sel T47D yang diberi perlakuan ekstrak kloroform P. macrocarpa dilihat dengan metode double stainning dengan etidium bromida-akridin oranye. Hasil penelitian menunjukkan nilai LC50Â sebesar 103,03 Î¼g/ml. ekstrak ini mempunyai efek antiproliferasi dan mampu memperpanjang waktu doubling time. Pengamatan apoptosis dengan double staining menggunakan etidium bromida-akridin oranye, menunjukkan dapat memacu apoptosis sel T47D.

OPTIMIZATION OF FLOATING TABLET CONTAINING ASPIRIN USING SIMPLEX LATTICE DESIGN Siswanto, Agus
Indonesian Journal of Pharmacy Vol 31 No 1, 2020 In Press
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss1pp%p

Aspirin is an effective analgesic which is also used as an antiplatelet therapy for stroke prophylaxis. Aspirin in floating tablet formulations is used to improve the bioavailability. The aim of this study was to determine the optimum composition of floating tablets of aspirin by simplex lattice design (Design Expert 7.1.5). The tablets were prepared by direct compression technique, using polimers (Methocel K4M CR, Ethocel) and sodium bicarbonate (gas-generating agent) as independent variables. The optimum formula was determined based on numeric method with flow rate of tablet mass, floating lag time, C60, and Td as independent variables. The results indicated that the optimum composition of the matrix was Methocel K4M CR 16.94 %, NaHCO3 15.18 %, and Ethocel 17.88 %. It was observed that independent factors had significant contribution on all dependent variables.

Uji Bioavailabilitas Tablet Floating Aspirin Siswanto, Agus
Jurnal Kefarmasian Indonesia VOLUME 7, NOMOR 2, AGUSTUS 2017
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v7i2.3498

Aspirin is a non-steroidal anti-inflammatory drug with potential as antiplatelet for stroke prophylaxis. Several approaches of aspirin formulations in various dosage forms have been performed. Formulations of aspirin in conventional tablet dosage form often cause gastric irritation. Aspirin is rapidly absorbed in the upper gastrointestinal tract, especially the first small intestine. Therefore formulation of floating drug delivery system are designed to improve the bioavailability of aspirin. The floating system will retain the tablet in stomach, allowing sufficient absorption time for aspirin in stomach and upper intestine. Aim of this study was to determine relative bioavailability of aspirin floating tablets compared to the aspirin enteric coated tablets in rabbits with crossover design method. Serial blood samples were collected from rabbit ear marginal vein over a 10-h period. Drugs concentration in plasma (aspirin and salisylic acid) were determined by HPLC with benzoic acid as internal standard. The results showed that the floating aspirin tablet has better bioavailability with shorter tmax and more uniform of aspirin levels compared to enteric coated tablets, though the parameters AUC and Cpmax both of those products were not significant (p> 0.05).

Validasi Metode HPLC untuk Penetapan Aspirin dan Asam Salisilat dalam Plasma Kelinci (Lepus curpaeums) secara Simultan Agus Siswanto; Achmad Fudholi; Akhmad Kharis Nugroho; Sudibyo Martono
Jurnal Kefarmasian Indonesia VOLUME 6, NOMOR 2, AGUSTUS 2016
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Aspirin is a nonsteroidal anti-inflammatory drug which also has the effect of antiplatelet for stroke prevention. Aspirin inside human body is very easy to break down into salicylic acid as the main metabolite. The aim of this study is to develop and validate the method for determinating aspirin and salicylic acid concentration in plasma by HPLC. Method validation including system suitability test, linearity test, determination of LOD and LOQ, recovery, accuracy and precision. Concentration of analytes in blood is measured by HPLC using benzoic acid as internal standard, with condition Purospher column Endcapped Star RP-18 (250 x 4.6 mm id, 5 m), acetonitrile : buffer phosphate 20 mM pH 2.5 (30:70 v/v) as mobile phase, injection volume 20 mL, flow rate 1.5 mL/minute, and UVVis detector λ 230 nm. The results showed that the proposed method meets the requirements of system suitability and good linearity (r > 0,990) with LOQ (aspirin = 0.024 mg/mL, salicylic acid = 0.336 mg/mL) and LOD (aspirin = 0.007 mg/mL, salicylic acid = 0.101 mg/mL). The method of analysis provides recovery of 85-115 %, accuracy and precision in accordance with the requirements for bioanalytical with CV < 5 %. Therefore, the proposed method is applicable to determine of aspirin and salicylic acid concentration in plasma.

Profil Distribusi Apotek di Kabupaten Banyumas berdasarkan Sistem Informasi Geografi dan Korelasinya dengan Jumlah Kunjungan dan Resep Tahun 2019 Abdul Manan; Pri Iswati Utami; Agus Siswanto
Jurnal Kefarmasian Indonesia VOLUME 11, NOMOR 2, AGUSTUS 2021
Publisher : Pusat Kebijakan Sistem Ketahanan Kesehatan dan Sumber Daya Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v11i2.3961

The distribution of pharmacies in Indonesia is still becoming a problem. Pharmacies are mostly available around healthcare facilities such as hospitals, service providers, trade centers, transportation facilities, and main roads. The study aims to describe the distribution profile of pharmacies based on Geographic Information System (GIS) analysis and to study the relation between pharmacy distribution profiles with the number of consumer visits and prescriptions. The study is conducted in 27 sub-districts in Banyumas Regency, Central Java using Arc Gis 3.10.2 Software. Pharmacy profile, the number of consumer visits, and prescriptions at the pharmacy are obtained by distributing research questionnaires to pharmacists. The results showed that the distribution of pharmacies in the Banyumas Regency was still dominant in urban areas, which reached 55%. The average distance of pharmacies was 2.34 km. The research results on the distribution pattern of pharmacies in 27 sub-districts showed that in 3 districts (11.1%) were grouped in certain areas, in 24 other districts (88.9%), the pharmacies' distribution had a spread distribution pattern. The conclusion of the research that the distribution of pharmacies in the Banyumas Regency based on GIS visualization was still dominant in urban areas and especially in sub-districts around the capital of Banyumas Regency, Purwokerto. Based on the study, there was a relation between the average distance of pharmacies and the number of healthcare facilities on consumer visits. There was a relation between pharmacy density, population density, and the number of healthcare facilities with the number of prescriptions.

Pemodelan Farmakokinetika Tablet Floating Aspirin pada Kelinci dengan PKSolver AGUS SISWANTO; ACHMAD FUDHOLI; AKHMAD KHARIS NUGROHO; SUDIBYO MARTONO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 2 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Aspirin selain sebagai analgetik juga efektif digunakan sebagai antiplatelet untuk profilaksis stroke. Aspirin diabsorpsi secara cepat di saluran pencernaan bagian atas terutama di bagian pertama usus halus. Formulasi dalam bentuk tablet floating diharapkan mampu memperbaiki profil farmakokinetika aspirin. Tujuan penelitian ini adalah untuk menentukan model farmakokinetika tablet floating aspirin pada kelinci dengan PKSolver. Penentuan model dengan PKSolver berdasarkan parameter: 1) visual goodness of fit (GOF) dan 2) parameter statistik yang meliputi Akaike’s information criteron (AIC) dan Schwarz criterion (SC). Berdasarkan kedua parameter tersebut menunjukkan bahwa model multiple site absorption (MAS) merupakan model terbaik untuk menjelaskan farmakokinetika tablet floating aspirin. Pelepasan dan absorpsi aspirin dari tablet floating terjadi melalui 2 tahap sehingga menghasilkan profil farmakokinetika dengan puncak ganda akibat absorpsi yang terjadi secara paralel di dalam lambung.

OPTIMASI TABLET FLOATING TEOFILIN MENGGUNAKAN METODE DESAIN FAKTORIAL agus siswanto; Indri Hapsari; yusni ulfiani zulaikha; eva rosmawati
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol. 3 No. 1 (2016)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.05 KB)

ABSTRACTTheophylline is a drug used in the management of asthma. It has relatively short half-life and narrow therapeutic index with 5-20 μg/mL serum concentration. Thus, Floating tablet formulations that can increase the bioavailability of the drug. The aims of this study were to determine the effect of the excipient (HPMC K4M CR and NaHCO3) to the quality of tablets and determine the optimum formula of theophylline floating tablet. A 22 randomized factorial design was used in development of theophylline floating tablet with HPMC K4M CR (A) and NaHCO3 (B) as independent variables.The flowability of mass tablet, floating character, and dissolution profile were chosen as dependent variables.The results showed that NaHCO3 was the most important factor on increasing flowability of mass tablet. HPMC K4M CR was the dominant factor in lowering the Flag time, improving integrity of the tablet, and decreasing the release of drug. The composition of HPMC K4M CR 40.00 mg and NaHCO3 35.39 mg produced the optimum formula of theophylline floating tablet with desirability value of 0.978.

Pemodelan Pelepasan In Vitro Tablet Floating Teofilin Dengan Program Ddsolver Agus Siswanto; Indri Hapsari; Yusni Ulfiani Zulaikha; Eva Rosmawati
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 13 No. 01 Juli 2016
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

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ABSTRAK Disolusi merupakan elemen penting dalam pengembangan obat terutama terkait dengan absorpsi dan bioavailabilitas sediaan. Penelitian ini bertujuan untuk menentukan kinetika pelepasan tablet floating teofilin secara in vitro. Tablet floating teofilin dibuat dengan metode granulasi basah menggunakan bahan tambahan HPMC K4M CR, NaHCO3, laktosa, dan magnesium stearat. Hasil evaluasi sifat fisik menunjukkan bahwa tablet floating teofilin mempunyai bobot yang seragam (CV=1,93%), kekerasan yang baik (8,4 kg), dan kerapuhan yang rendah (0,05%). Evaluasi model pelepasan dengan program DDSolver menggunakan 2 kriteria: 1). Parameter statistik: R2adjusted, mean square error (MSE), weighted sum of squares (WSS), akaike information criterion (AIC), model selection criterion (MSC); 2). Visual goodness of fit (GOF). Hasil evaluasi data disolusi dengan program DDSolver menunjukkan bahwa pelepasan tablet floating teofilin mengikuti persamaan Weibull. Kurva disolusi berbentuk eksponensial dan pelepasan teofilin terjadi melalui mekanisme difusi. Kata kunci: tablet floating teofilin, DDSolver, pemodelan pelepasan obat. ABSTRACT The dissolution is an important element in drug development is mainly related to the absorption and bioavailability. The aim of this study was to determine in vitro dissolution study of theophylline floating tablet release kinetics model. The floating tablets were prepared by a wet granulation method with HPMC K4M CR, NaHCO3, lactose, and magnesium stearate as excipients. The result showed that the theophylline floating tablets had a weight uniformity (CV=1.93%), good hardness (8.4 kg), and low friability (0.05%). Dissolution data were evaluated using DDSolver conducted by 1). Statistical parameters: R2adjusted, mean square error (MSE), weighted sum of squares (WSS), akaike information criterion (AIC), model selection criterion (MSC); 2). Visual goodness of fit (GOF). The results showed that theophylline floating tablets release kinetics followed the Weibull equation. The dissolution curve is exponential shape and theophylline release occurs through a diffusion mechanism. Key words: floating tablet of theophylline, DDSolver, modeling of drug release.

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