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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Matematika & Sains JSKK (Jurnal Sains Keolahragaan dan Kesehatan) Pharmacology and Clinical Pharmacy Research Jurnal Kefarmasian Indonesia
Slamet Ibrahim
Sekolah Farmasi, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Health Professions Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Nursing Public Health

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Journal : Jurnal Teknologi Dan Industri Pangan

 1 
Determination of Iodate and Iodide Content in Iodized Salt By Ion Pair High Performance Liquid Chromatography Method Wisnu Cahyadi; Kurnia Firman; Slamet Ibrahim; Embit Kartadarma
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Two species of iodine, i.e. iodide and iodate in commercial iodized salt were determined using ion pair HPLC. From 15 samples analysed, the iodide and iodate content ranged from 24,05 ± 2,51 to 70,25 ± 3,78 ppm and from 31,43 ± 8,10 to 87,59 ± 0,44 ppm, respectively. The method used was found satisfactory in terms of precission, accuracy, sensitivity and selectivity, therefore the method seem acceptable for the determination of iodide and iodate content in iodized salt samples. Key words : Iodine species, determination of iodine species content, and ion-pair HPLC
SENYAWA ASAM 2- METILESTER-1-H-PIROL-4-KARBOKSILAT DALAM EKSTRAK ETIL ASETAT BUAH SALAK VARIETAS BONGKOK SEBAGAI ANTIOKSIDAN DAN ANTIHYPERURICEMIA [Studies on 2-Methylester-1-H-Pyrolle-4-Carboxylic Acid Compound in Ethylacetate Extract of Snake Fruit Var Leni Herliani Afrianti; Elin Yulinah Sukandar; Slamet Ibrahim; I Ketut Adnyana
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The aim of the study was to determine the antioxidant and antihyperuricemia activity of ethyl acetate extract of snake fruit (Salacca edulis Reinw.) var. Bongkok. The research methods used in this study comprised  of three stages. First stage, the isolation processes, consist ed of maceration, fractionation, and purification using several techniques of chromatography. The chemical structures of the isolated compounds were determined based on UV, IR, 1-D NMR, and 2-D NMR spectral data. The ethyl acetate extract of snake fruit var. Bongkok isolated was a new compound 2-methylester-1-H-pyrolle-4- carboxylic acid. In the second stage the antioxidant activity of the extract and the isolated compounds were measured by 1,1 diphenol  (DPPH) method. The antioxidant activity of the extracts and the isolated compounds were expressed as IC50, The ethyl acetate extracts at concentrations of 0.2, 2, 20, 200, 400, and 2000 µg/mL showed inhibition of 9.67, 4.47, 41.89, 96.06, 82.54, and 90.60 % respectively, with an IC50 of 1.6 µg/mL. Ascorbic acid standards at the same concentration range showed an  IC50 of 0.54 µg/mL. Meanwhile, at the same concentrations the 2-methylester-1-H-pyrolle-4-carboxylic acid showed free radical inhibition of 17.48, 21.48, 18.14, 31.87, and 62.34 % respectively, with an  IC50 of 3.27  µg/mL. During the third stage,  the antihyperuricemic properties of the extracts and the isolated compound were examinated in vitro using inhibition of xanthin oxidase method. The ethyl acetate extracts at concentrations of 0.01, 0.02, 0.2, 2, and 2000  µg/mL showed xanthin oxidase inhibition of 49.24, 49.58, 50.28 and 52.26 % respectively, with an  IC50 of 24.75 µg/mL. At the same concentrations, the 2-methylester-1-H-pyrolle-4- carboxylic acid, showed xanthin oxidase inhibition of 27.7, 30.5, 37.3, 50.27 and 50.55 % respectively, with an IC50 of 48.86 µg/mL.  Allopurinol as a standard drug showed an IC50 of 0.92 µg/mL.
Co-Authors Abdul Mutalib Amir Mursadad As'ari Nawawi Dea Dwi Puspita Eli Halimah Elin Yulinah Sukandar Embit Kartadarma Fauzan Fikri Hidehiro Uekusa I Ketut Adnyana Ilma Nugrahani Jutti Levita Kurnia Firman Leni Herliani Afrianti Nyi M. Saptarini Nyi M. Saptarini Okky Dwichandra Putra Pusparani Krisnamurthi Rachmat Mauludin Saeful Amin Saeful Amin, Saeful Sophi Damayanti Sri A. Sumiwi Wisnu Cahyadi