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All Journal JURNAL TEKNOLOGI LINGKUNGAN Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Seruni - Seminar Riset Unggulan Nasional Inoformatika dan Komputer Konversi BERITA BIOLOGI JURNAL BIOLOGI INDONESIA JURNAL PERTANIAN Jurnal Leverage, Engagement, Empowerment of Community (LeECOM)
Joko Sulistyo
Bagian Teknologi Hasil Hutan, Fakultas Kehutanan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Engineering Environmental Science Social Sciences Veterinary

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Pembuatan Education Game Tenses Pada Sekolah Menengah Pertama Negeri 2 Boyolali Sulistyo, Joko
Seruni - Seminar Riset Unggulan Nasional Inoformatika dan Komputer Seruni 2012 - Seminar Riset Unggulan Nasional Informatika dan Komputer
Publisher : Seruni - Seminar Riset Unggulan Nasional Inoformatika dan Komputer

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Abstract

Abstraksi: Kendala terbesar bagi siwa SMP Negeri 2 Boyolali Kelas VII dalam belajar bahasa inggris adalah penggunaan tenses. Banyak dari mereka yang tidak memahami dan menguasai penggunaan tenses. Bahkan mereka kesulitan dalam pemilihan kata kerja yang sesuai dengan waktu kejadiannya. Selain itu guru dalam menyampaikan materi juga masih bersifat konvensional yaitu hanya menggunakan papan tulis dan spidol. Selain itu penyampaiannya pun hanya satu arah tanpa adanya interaksi dari siswa. Sehingga siswa menjadi jenuh dalam belajar bahasa inggris khusunya belajar tenses. Agar semua siswa khususnya di SMP Negeri 2 Boyolali lebih menguasai present tenses, past tenses maupun future tenses maka salah satunya dengan pembuatan game tentang penggunaan kata kerja dalam present tenses, past tenses maupun future tenses yang dapat digunakan sebagai media pembelajaran mandiri. Adapun penulis dalam hal ini menggunakan metode kepustakaan, observasi, studi analisis, pengambilan data, perancangan sistem, uji coba dan implementasi. Hasil Penelitian ini ini berupa terciptanya Education Game Tenses Pada Sekolah Menengah Pertama (SMP) Negeri 2 Boyolali
PENGARUH PENGGUNAAN NANAS DAN UMBI POHON GADUNG SEBAGAI KOAGULAN TERHADAP KUALITAS BAHAN OLAHAN KARET RAKYAT Praharnata, Praharnata; Sulistyo, Joko; Wijayanti, Hesti
Konversi Vol 5, No 1 (2016): April 2016
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/k.v5i1.4776

Abstract

Abstrak- Prakoagulasi lateks merupakan penggumpalan spontan lateks yang biasanya disebabkan oleh pengaruh asam, enzim dan bakteri. Bahan yang digunakan untuk mempercepat prakoagulasi sering dikenal sebagai koagulan. Beberapa penduduk di Kalimantan Selatan menggunakan beberapa koagulan alami yang tidak banyak dikenal seperti umbi akar pohon gadung dan tongkol nanas, sehingga dalam penelitian ini kami ingin mengkaji lebih dalam mengenai penggunaan koagulan alami ini, mengamati dan membandingkan kualitas karet yang dihasilkan. Percobaan dilakukan dengan melakukan pengamatan pada sampel lateks yang telah dicampurkan dengan koagulan yang telah divariasi, penelitian ini mencoba meniru cara pengolahan karet remah kering, sehigga sampel karet yang dibuat memiliki sifat teknis yang dapat diuji. Adapun uji yang dilakukan dalam penelitian ini antara lain; uji prakoagulasi dengan koagulan yang bervariasi, uji prakoagulasi dengan dosis koagulan yang divariasikan, uji prakoagulasi lateks dengan air sisa prakoagulasi awal dan uji sifat teknis karet. Dari hasil analisa sampel gadung diperoleh kadar nitrogen karet sebesar 0,22 %, Po 31, dan PRI 76, pada sampel nanas diperoleh kadar nitrogen sebesar 0,24%, Po 21,5 dan PRI 56. Kata kunci: Prakoagulasi, lateks, koagulan, enzim, bakteri Abstract- Latex precoagulation is a spontaneous clot of latex that is usually caused by the effects of acids, enzymes and bacteria. Materials used to make precoagulation faster known as a coagulant. Some people in South Kalimantan using some natural coagulant that is not widely known as a gadung tree root and pineapple cob, so in this study we wanted to examine more deeply about the use of this natural coagulant, observe and compare the quality of rubber produced. The experiments were performed by making observations on a sample of latex was mixed with a coagulant which has been varied, this study tried to imitate the dry crumb rubber processing, so the rubber samples that created has technical properties that can be tested. The tests performed in this study were precoagulation test with varying coagulant, precoagulation test with coagulant dose varied, latex precoagulation test with waste water of early precoagulation and test of the technical properties of the rubber. From the analysis of gadung sample  obtained rubber nitrogen content of 0.22 %, Po 31, and the PRI 76, the pineapple samples obtained rubber nitrogen content of 0.24 %, 21.5 Po and the PRI 56. Keywords: Precoagulation, latex , coagulants , enzymes , bacteria  
Biological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth Soeka, Yati Sudaryati; Widiasih, Lutfi Erlita; Sulistyo, Joko; Joko Sulistyo
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.37 KB) | DOI: 10.14203/jbi.v3i3.3467

Abstract

ABSTRACTBiological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth. We have studied an indigenous bacterial strain produced a glycosyl transfer enzyme (CGTase) yielding polyphenol glycosides from a substrate of starch and polyphenol-aglycone. We observed that the CGTase derived from culture filtrate of some microbial strains (Candida rugosa, Bacillus megaterium, B. coagulans and B. polymixa) could synthesize transfer products in the presence of appropriate polyphenol-aglycones as their acceptors. An inhibitory effects of enzymatically synthesized polyphenol glycosides against bacterial growth was furthermore examined. It was found that polyphenol-glycoside, as one of the transfer products, exhibited high antibacterial activity on the growth of Bacillus subtilis and Escherichia coli, no effect when on Bacillus cereus.Key words : Cyclodextrin glucano transferase (CGTase), enzymatic transglycosylation,polyphenol glycoside, antibacterial activity.
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) [Bioprocessing of Fermented Coconut Oil by Application of Fermentation Technology] Joko Sulistyo; Sulistyo, Joko; Soeka, Yati Sudaryati; Triana, Evi; Napitupulu, Rostiati NR
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.461 KB) | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.
AKTIVITAS ANTIMIKROBA FLAVONOID - GLIKOSIDA HASIL SINTESIS SECARA TRANSGLIKOSILASI ENZIMATIK Soeka, Yati Sudaryati; Naiola, Elidar; Sulistyo, Joko; Joko Sulistyo
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.43 KB) | DOI: 10.14203/beritabiologi.v8i6.825

Abstract

Flavonoid-glycoside was synthesized enzymatically using CGT-ase (EC.2.4.1.19) of indigenous Bacillus licheniformis in a phosphate buffer pH 6.0 at 45°C for 24 h, through transglycosylation reaction in the present of flavonoid those were extracted from rhizomes such as ginger, flngerroot, turmeric, white turmeric and white curcuma as natural acceptors, and commercial rice,cassava, corn and wheat flour as substrates.The result showed that CGT-ase of B. licheniformis transferred a glycosyl moiety in a bilayer enzymatic reaction system of n-hexanol and phosphate buffer yielding glycosides as transfer products in the present of wheat flour as substrate and white curcuma extract as its acceptor.An inhibitory effects of the synthesized flavonoid glycosides against microbial growth was furthermore examined. It was found that flavonoid-glycoside, as the transfer product, exhibited high antimicrobial activity at MIC 200 ppm on the growth of Bacillus subtilis, Escherichia coli and Saccharomyces cerevisiae, however no effect when it was assayed on Candida tropicalis, while arbutin and flavonoid-aglycon showed very low inhibitory activity on the growth of two out of four tested microbial strains.
DEVELOPMENT OF PURE CULTURE STARTER FOR KECAP, AN INDONESIAN SOY SAUCE [Pengembangan Ragi Kecap Khas Indonesia Dengan Biakan Murni] Joko Sulistyo; Sulistyo, Joko; Nikkuni, Sayuki
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.719 KB) | DOI: 10.14203/beritabiologi.v7i6.863

Abstract

In order to prevent aflatoxin contamination during the production of kecap, an Indonesian soy sauce, a white-spored mutant K-IA strain induced from an aflatoxin-negative koji mold was applied to koji making process of kecap. While kecap koji making process took 9 days by conventional method without inoculum, the use of K-IA strain as a starter shortened the process to 3 days at room temperature. The koji prepared with this starter contained 2.3 x 107 cfu/g of Aspergillus sp. K-IA as the dominant speciesand some contaminants from the environment such as other species of Aspergillus (2.0 x 10 cfu/g) and Mucorales (1.4 x 10s cfu/g). The mash prepared with kecap koji inoculated with this starter contained higher formol nitrogen (FN) and water-soluble nitrogen (WN) than those prepared with koji inoculated without any starter.These results demonstrated that the use of this starter did not only contribute to the prevention of aflatoxin contamination but also improved the conventional kecap fermentation process.
PERTUMBUHAN DAN KELANGSUNGAN HIDUP BENIH IKAN GURAMI (Osphronemus gouramy) PADA SISTEM RESIRKULASI DENGAN PADAT TEBAR 5,7 DAN 9 EKOR/LITER Sulistyo, Joko; Muarif, Muarif; Mumpuni, Fia Sri
JURNAL PERTANIAN Vol 7, No 2 (2016)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.73 KB) | DOI: 10.30997/jp.v7i2.21

Abstract

Ikan gurami (Osphronemus gouramy) merupakan komoditas unggulan ikan air tawar, tetapihasil produksinya masih belum dapat memenuhi permintaan pasar. Oleh karena itu perluupaya dan teknologi untuk meningkatkan ketersediaan benih dan produksi benih ikan guramedengan pembenihan secara intensif melalui peningkatan padat penebaran dengan sistemresirkulasi. Penelitian ini bertujuan untuk mengetahui pertumbuhan dan kelangsungan hidupbenih ikan gurami terbaik dengan padat penebaran 5, 7 dan 9 ekor/liter, dengan ukuran benih1 cm dengan sistem resirkulasi. Hasil penelitian ini menunjukkan perlakuan padat penebaranbenih ikan gurami yang berbeda dalam pemeliharaan sistem resirkulasi memberikan pengaruhnyata terhadap derajat kelangsungan hidup, laju pertumbuhan bobot harian, dan panjangmutlak. Nilai tertinggi derajat kelangsungan hidup diperoleh pada padat tebar 5 ekor/literdengan nilai 83,85%, sedangkan nilai laju pertumbuhan bobot harian dan pertumbuhanpanjang mutlak tertinggi diperoleh pada perlakuan padat tebar 7 ekor/liter.Kata kunci: padat penebaran, ikan gurame, tingkat pertumbuhan, tingkat kelangsungan hidup
ANALISA ASAM ORGANIK DAN ASAM AMINO PADA NATTO YANG DIFERMENTASI OLEH 7 STRAIN BACILLUS NATTO Joko Sulistyo; SULISTYO, JOKO
BERITA BIOLOGI Vol 3, No 6 (1986)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.539 KB) | DOI: 10.14203/beritabiologi.v3i6.1326

Abstract

JOKO SUUSTYO. 1986. Analysis of organic acid and amino acid in natto fermented by 7 strains of Bacillus natto. Berita Biologi 3 (6): 277 - 282. The ability of seven strains of Bacillus natto produced a number of organic and amino acid components in natto were investigated. Raw, submerced and autoclaved soybeans for natto contained 1.15% 0.46% and 0.77% in organic acid and 0.19%, 0.30% and 0.30% in amino acid, while after fermentation those range were 0.36 - 0.90% in organic acid and 2.86 -6.08% in amino add. During submercing, autoclaving and fermenting, citric acid component decreased down very sharply to about one hundredth of starting level. While acetic,iso-butiric and iso-valeric components increased up to 84.92%, 96.60% and 95.87%, respectively. Mean while, glutamic acid, iso-leucine,leucine andphenylalanine components of amino acid increased up to 84.36%, 90.52%, 91.67% and 95.88%, respecti
Aktivitas Antimutagen Isoflavon Glikosida Hasil Transglikosilasi Enzimatik CGT-ase Bacillus macerans Sulistyo, Joko; Soeka, Yati Sudaryati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.276 KB) | DOI: 10.24002/biota.v14i1.2629

Abstract

It has been known that isoflavone have biological activities such as antioxidant, antibacteria, antimutagenesis, and anticancer. Isoflavone aglycone uses such as genistein, daidzein and glycitein are limited since they are unstable and uneasily to dissolve in water. Through enzymatic transglycosylation reaction, its stability and solubility could be improved. In this study, genistin (isoflavone glycoside) was synthesized from genistein (isoflavone aglycone) by application of transfer reaction using enzyme cyclodextrin glucanotransferase (CGT-ase) of Bacillus macerans. Identification of the product was determined by TLC with methanol: chloroform (1:3, v:v) as eluent. Rf value 0.75 of the synthesized product was close to the Rf value standard of authentic genistin glycoside. The synthesized genistin was furthermore assayed to determine its antimutagenesis activity according to the Ames methode on E. minimal glucose media had been precultured with a mutant strain of Salmonella thypimurium TA98. The tested bacterial strain was induced with aflatoksin B1 as mutagen which had been activated with a lever homogenate. The result showed that the solubility and some biological properties of the synthesized genistin were improved higher than that of genistein, while it was found to be lower than that of the commercial genistin.
SEMI PURIFIKASI DAN UJI REAKSI TRANSGLUKOSILASI P-GLUKOSIDASE DARI ASPERGILLUS PULVERULENTUS Sulistyo, Joko; Soeka, Yati Sudaryati; Dewi, Purnama
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1263

Abstract

An extracellular p-glucosidase[EC 3.2.1.21]derived from Asvereillus vulverulentus were separated and partially purified by successive chromatographies and its some characterization and transglucosylation capacity were studied.The purification protocol included precipitation with ammonium sulphate,gel filtration,ion exchange chromatography and native polyacrylamide gel electrophoresis.The enzyme readily hydrolysed cellobiose to form transglucosylation products in the present of primary alcohol acceptors.This P-glucosidase was stable at temperatures up to 70 °C and from pH 2.5 to 8.5, while its highest activity was in the pH 4.5 at 65°C.
Co-Authors Evi Triana Fia Sri Mumpuni Hesti Wijayanti Irra Chrisyanti Dewi Joko Sulistyo Muarif, Muarif Naiola, Elidar Napitupulu, Rostiati NR Nikkuni, Sayuki Praharnata, Praharnata Purnama Dewi R. Sapto Hendri Boedi Soesatyo Rini Handayani Rita Dwi Rahayu Widiasih, Lutfi Erlita Widiasih, Lutfi Erlita Yati Sudaryati Soeka Yoewono, Jessica Renata