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All Journal Jurnal Sains dan Seni ITS Jurnal Teknologi Pangan Plumula : Berkala Ilmiah Agroteknologi Wahana-Bio: Jurnal Biologi dan Pembelajarannya Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JURNAL AGROHITA Agro Bali: Agricultural Journal Nusantara Science and Technology Proceedings Berkala Penelitian Hayati
Sutini
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Pembangunan Nasional
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Other

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Journal : Jurnal Teknologi Pangan

 1 
PRODUKSI EPIGALLOCATECHIN GALLATE PADA KULTUR IN VITRO KALUS CAMELLIA SINENSIS SEBAGAI KANDIDAT PANGAN FUNGSIONAL Basuki, Sutini
REKAPANGAN Vol 5, No 2 (2011): REKAPANGAN
Publisher : UPN VETERAN JAWA TIMUR

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Epigallocatechin gallate (EGCG)  were  secondary metabolite in  tea  (Camellia sinenis)  as  anti  obecity dan  degenerative  syndrom.  EGCG  in  tea  act  as  bioactive  by hydroxyl group and galoil that could act as functional food substitute. Problem to find epigallocatechin gallate from tea were season dependent, need a large area, and low productivity.  By  these  mean epigallocatechin  gallate  production  by  in  vitro  culture. These technique could overcaming all that problem above. General purpose of these research  were  to  find invitro  EGCG  production technique.  Method  to  gain  these purpose were : (1)  Callus induction by explant sprout tea leaves in media  with many variance  grown  culture (2)  Sub  culture  callus  by  using  same  media  in  induction method  (3) qualitative  analisys EGCG  callus compound. Result  of  these  research; callus  hand retention  time  (tR)  on  High  Performance  Liquid  Chromatography  for  6 minutes, same as EGCG standart. EGCG had potential as aditive functional food and baverage  that had astringent or bitter tasteKey words : Epigallocatechin gallate,  HPLC, retention time, Camellia sinensis
IDENTIFIKASI POLIFENOL PADA KULTUR IN VITRO KALUS Camellia sinensis UNTUK BAHAN MINUMAN FUNGSIONAL Basuki, Sutini
REKAPANGAN Vol 6, No 1 (2012): REKAPANGAN
Publisher : UPN VETERAN JAWA TIMUR

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Polyfenol  included  secondary  bioactive  metabolite  had  a  function  to  reduce  degenerative syndrome. Polyfenol contained in tea act as bioactive syntesis from hydroksil part and could be act as functional drinking and  control human organ as biological metabolism. Problem in  gain polyfenol productfrom tea plantation / camellia sinensis L  for intance : depent on season, need  a large area for  cultivication,  and  low  contain  for  productivity.For  thesepurpose  there  were  main  idea  to ncrease polyfenol  productivity  by  mean  of  in  vitro  culture  technique.  These  technique  could  reduce  all problem  above.  The  main  purpose  of  these  research  were  to  find  production  technique  by  mean callus  culture.    Method  to  gain  the  purose  were  :  (1)  callus  induction  by  cultify  part  of tea  leaves  in many variance  grown culture , (2)  Sub culture callus  using same culture  media  as callus   induction (3)  Morphologycal  check  for  callus  (4)  Kualitative  analisys  callus  polyfenol.Result  of these  research were  callus with hand time retention (tR) on High Performace Liquid Chromatography for 14 minutes with a same time of standart poyfenol.  Polyfenol had potential to support  the retention of functional beverage.Key words : Polyphenols,  in vitro culture of  callus , Camellia sinensis
Co-Authors Agus Purwanto Alvhi Widya Calandry Alvriado Igo Fransiscus Siahaan Augustien, Nora Ayu Silviana Carolina Rosa Siallagan Didik Utomo Pribadi Djoko Agus Purwanto Felicitas Deru Dewanti Guniarti Guniarti Guniarti Guniarti Hadi Suhardjono Juli Santoso M. Rasjad Indra Milatur Royyana Muslihatin, Wirda Nora Agustien Nora Augustien Nora Augustien Pribadi, Didik Huswo Pribadi, Didik Utomo Ramadhani Mahendra Kusuma Shofiyah Zanirah Sukendah Sukendah Susilowati Susilowati Sutiman B. Sumitro Tatik W Wahyu W Widiwuriani Widiwurjani Zulfikar Alvin Naufal