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All Journal Jurnal Sains dan Seni ITS Jurnal Teknologi Pangan Plumula : Berkala Ilmiah Agroteknologi Wahana-Bio: Jurnal Biologi dan Pembelajarannya Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JURNAL AGROHITA Agro Bali: Agricultural Journal Nusantara Science and Technology Proceedings Berkala Penelitian Hayati
Sutini
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Pembangunan Nasional
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Other

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PRODUKSI EPIGALLOCATECHIN GALLATE PADA KULTUR IN VITRO KALUS CAMELLIA SINENSIS SEBAGAI KANDIDAT PANGAN FUNGSIONAL Basuki, Sutini
REKAPANGAN Vol 5, No 2 (2011): REKAPANGAN
Publisher : UPN VETERAN JAWA TIMUR

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Abstract

Epigallocatechin gallate (EGCG)  were  secondary metabolite in  tea  (Camellia sinenis)  as  anti  obecity dan  degenerative  syndrom.  EGCG  in  tea  act  as  bioactive  by hydroxyl group and galoil that could act as functional food substitute. Problem to find epigallocatechin gallate from tea were season dependent, need a large area, and low productivity.  By  these  mean epigallocatechin  gallate  production  by  in  vitro  culture. These technique could overcaming all that problem above. General purpose of these research  were  to  find invitro  EGCG  production technique.  Method  to  gain  these purpose were : (1)  Callus induction by explant sprout tea leaves in media  with many variance  grown  culture (2)  Sub  culture  callus  by  using  same  media  in  induction method  (3) qualitative  analisys EGCG  callus compound. Result  of  these  research; callus  hand retention  time  (tR)  on  High  Performance  Liquid  Chromatography  for  6 minutes, same as EGCG standart. EGCG had potential as aditive functional food and baverage  that had astringent or bitter tasteKey words : Epigallocatechin gallate,  HPLC, retention time, Camellia sinensis
IDENTIFIKASI POLIFENOL PADA KULTUR IN VITRO KALUS Camellia sinensis UNTUK BAHAN MINUMAN FUNGSIONAL Basuki, Sutini
REKAPANGAN Vol 6, No 1 (2012): REKAPANGAN
Publisher : UPN VETERAN JAWA TIMUR

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Abstract

Polyfenol  included  secondary  bioactive  metabolite  had  a  function  to  reduce  degenerative syndrome. Polyfenol contained in tea act as bioactive syntesis from hydroksil part and could be act as functional drinking and  control human organ as biological metabolism. Problem in  gain polyfenol productfrom tea plantation / camellia sinensis L  for intance : depent on season, need  a large area for  cultivication,  and  low  contain  for  productivity.For  thesepurpose  there  were  main  idea  to ncrease polyfenol  productivity  by  mean  of  in  vitro  culture  technique.  These  technique  could  reduce  all problem  above.  The  main  purpose  of  these  research  were  to  find  production  technique  by  mean callus  culture.    Method  to  gain  the  purose  were  :  (1)  callus  induction  by  cultify  part  of tea  leaves  in many variance  grown culture , (2)  Sub culture callus  using same culture  media  as callus   induction (3)  Morphologycal  check  for  callus  (4)  Kualitative  analisys  callus  polyfenol.Result  of these  research were  callus with hand time retention (tR) on High Performace Liquid Chromatography for 14 minutes with a same time of standart poyfenol.  Polyfenol had potential to support  the retention of functional beverage.Key words : Polyphenols,  in vitro culture of  callus , Camellia sinensis
In Vitro Culture Technology for the Production of Secondary Metabolites and the Development of Plants That are Resistant to Climate Change Sutini; Widiwurjani; Augustien, Nora; Guniarti; Pribadi, Didik Huswo; Purwanto, Djoko Agus; Muslihatin, Wirda
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0604

Abstract

Climate change that we are experiencing today is triggered by global warming which causes air temperature to rise, drought-land degradation, fluctuating rainfall, floods, landslides, pollution, and rising sea levels. In the agricultural sector, this results in decreased soil fertility, decreased agricultural yields, physiological effects on plants, weed disturbances and attacks by pests. Therefore, the estimation strategy and the provision of adaptive technology include applying plant-invitro culture technology that can adapt to climate change. The problem of this paper is the decrease in productivity and the stagnation of secondary metabolite biomass production in plants. This problem can be overcome by adapting in vitro culture techniques to produce secondary metabolites and plant development. In vitro culture has advantages including it does not depend on climate, altitude, weather and environmental temperature. The aim of this study was to adapt the in vitro plant culture techniques to obtain secondary metabolites and adaptive plant development. The method used is a review of the literature related to in vitro culture techniques that can produce secondary metabolite biomass and the development of various plant varieties. The conclusion of this research is that in vitro culture techniques can produce a variety of secondary metabolites and adaptive plants. The implication of this paper is to obtain a variety of secondary metabolite biomass and various plants that are resistant to climate change.
PERANAN ZAT PENGATUR TUMBUH ALAMI DALAM INDUKSI PEMBUNGAAN DAN PRODUKSI BERBAGAI VARIETAS TANAMAN CABAI (Capsicum annum L.) Alvriado Igo Fransiscus Siahaan; Sukendah Sukendah; Sutini Sutini
Plumula : Berkala Ilmiah Agroteknologi Vol 9 No 1 (2021): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v9i1.42

Abstract

Cayenne pepper (Capsicum annum L.) is a vegetable commodity that has received a lot of attention because it has a high economic value and the need for chili continues to increase every year in line with the increasing population and the development of industries that need chili as raw materials. One of the efforts to increase flowering induction and chilli production is by using natural plant growth from young corn and different types of chili varieties. This research was conducted from February to July 2019, which was carried out in the Karangploso farmer group land, East Java. This research is a factorial experiment that uses 2 factors compiled using a Completely Randomized Design (CRD). The first factor is a natural plant growth (with consentration 0, 3, 6, and 9, ml/L) and the second factor is a variety of chili varieties (Maruti, Santika, and Jalapeno). The results of the study showed that there were interactions on the parameters of the number of leaves, number of flowers, number of chilies, and weight of chili per plant. natural growth regulators of young corn extract affect plant growth and production on the variable number of leaves, number of flowers, number of chilies, and weight of chili per plant with different results, as well as the second factor, the type of Maruti variety shows the best results on the parameter number of leaves, and the number of flowers, Santika varieties showed the best results on the parameters of the number of flowers and the number of fruit per plant, while the Jalapeno variety did not show a significant effect.
Produksi Benih Sintetik Teh Camellia sinensis Alvhi Widya Calandry; Wirdhatul Muslihatin; sutini sutini
Jurnal Sains dan Seni ITS Vol 6, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.331 KB) | DOI: 10.12962/j23373520.v6i2.25415

Abstract

Teh (Camellia sinensis) memiliki kandungan dan manfaat yang banyak, sehimgga diperlukan alternatif untuk mendapatkan benih teh dalam waktu yang singkat dan dalam jumlah banyak. Salah satu alternatif adalah dengan melakukan perbanyakan tanaman secara vegetatif melalui pendekatan bioteknologi yaitu teknik Kultur In Vitro dan teknik enkapsulasi embriosomatik yang dapat menghasilkan benih sintetik. Tujuan dari penelitian ini untuk produksi benih sintetik dari embriosomatik guna penyediaan benih yang dapat disimpan dalam waktu yang lama. Benih sintetik Teh dengan bahan tanam embriosomatik yang dienkapsulasi menggunakan alginat 4% sehingga kapsul tidak terlalu padat dan memungkinkan benih dapat tumbuh dan berkecambah. Karakteristik benih sintetik yang dihasilkan yaitu tekstur benih sintetik padat namun tidak terlalu keras, masih rentan rusak apabila tidak hati-hati ketika memindahkan.. Benih sintetik berwarna hijau kekuningan dengan diameter 5,5 – 5,7 mm dan berat basah 260-290 mg.
In vitro culture technique of Camellia sinensis L for epicatechin production with phosphor inducer Sutini Sutini; Widiwurjani Widiwurjani; Nora Augustien; Hadi Suhardjono; Guniarti Guniarti; Djoko Agus Purwanto; Wirdhatul Muslihatin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.457 KB) | DOI: 10.23869/42

Abstract

In several ASEAN countries, in vitro culture techniques are one of the cultivation technologies that applied in sustainable agriculture. This technology has been performed in response to obtain the secondary metabolite of epicatechin from Camellia sinensis L. However, The yield of epicatechin is remaining low than in the field production. To overcome this problem, phosphor inducers is desirable to be supplemented in the study. The young leaf shoots of C. sinensis L was sliced on a culture-filled bottle, the sliced part was re-cultivated in the same medium by supplementing of 2,4-D growth regulators, then the medium was optimized by phosphor inducer with range of concentartions 40-200 ppm. The callus formation was then evaluated using high performance liquid chromatography methods to obtain qualitative and quantitative data. The results showed that the yield of epicatechin was obtained to 11.75%, suggesting that the supplementing of phosphor inducers at cultivation of in vitro culture of C. sinensis L able to improve the production of epicatechin by in vitro culture technique.
Growth and accumulation of flavan-3-ol in Camellia sinensis through callus culture and suspension culture method Sutini Sutini; Susilowati Susilowati; M. Rasjad Indra; Djoko Agus Purwanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (689.625 KB) | DOI: 10.23869/73

Abstract

This study was aimed to assess flavan-3-ol biomass in C. sinensis through callus cultures and suspension cultures derived from leaf explants. Callus initiation of both cultures were using Murashige and Skoog medium were enriched with plant growth regulators Naphthalene Acetic Acid 3.0 mg/L and kinetin 2.0 mg/L. The procedures in this study were: (1) callus initiation by cutting the leaves of C. sinensis shoots then planted on Murashige and Skoog medium that were enriched with plant growth regulators, (2) sub callus culture on fresh medium that enriched with the same growth regu-lators, (3) suspension culture initiation of liquid callus, (4) growth examination of callus and suspension cultures in week 12, (5) examination of qualitative-quantitative content of flavan-3-olin suspension cultures at week 4. The results show that suspension cultures contain biomass flavan-3-ol that increase in the same manner of the increase of callus age and weight.
MENINGKATKAN PRODUKSI FLAVAN-3-OL MELALUI KALUS Camellia sinensis L. DENGAN ELISATOR CU2+ Sutini B; Tatik W; Wahyu W; Sutiman B. Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/297

Abstract

Flavan-3-ol is a secondary metabolite in tea plant that is used as anti obesity agent. The difficulty in obtaining Flavan-3-ol out of tea plant is due to dependency on season, need for large land, need for very intensive care and relatively low production. Therefore flavan 3-ol production needs tobe developed in vitro culture technique. This technique can cope with the handicapes above. It can effectively control production and requires less space. The purpose of this research was to enhance fl avan 3-ol production by modifyingmedium and precursor appropriately. The steps of this process were: (1) Callus induction through cultivating the tea shoot explants on medium fi lled with some growth hormone, (2) fl avan-3-ol induction on callus culture using Cu 2+ elicitor. (3) Observation over callus growth, (4) Observation over qualitative characterizes of flavan 3-ol. The result of the research using Cu 2+ elicitor shows that the production of flavan-3-ol increases by 12.5%.
The Role of In Vitro Cultute Methods of Cammelia sinensis L. Plants in the Global Society Transformation Sutini; Widiwuriani; Guniarti; Agus Purwanto
Nusantara Science and Technology Proceedings 4th International Seminar of Research Month
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2019.0439

Abstract

The purpose of this paper is to explore the role of Cammelia sinensis L in vitro culture in the transformation of a global society with all its changes, both in positive terms and weaknesses. What makes this study relevant to this goal is the transformation of the global society, besides obtaining benefits, it also raises various problems. Problems that arise include the explosion of the world population due to climate change, the threat of malnutrition, the emergence of obesity disorders, disruption of agricultural production and chaos or the high cost of transportation whether by land, sea, or air. The method used is examining the literature on the role of in vitro culture in global social transformation related to climate change, health, agriculture, and transportation. The results obtained from the study of the role of in vitro culture in this global society transformation include obtaining some seeds with certain clones that are resistant to climate change so that nutritional deficiencies can be anticipated, secondary metabolites obtained from Cammelia sinensis L can be applied to reduce obesity. And in vitro culture can facilitate transportation because of the packaging form model from practical-efficient. This study concludes that the in vitro culture method of Cammelia sinensis L is very important in reducing problems in the global society transformation.
Production of Sweet Corn (Zea mays saccharata Sturt) Bonanza Variety on Planting Distance Setting and The Use of NPK Fertilizer Carolina Rosa Siallagan; Sutini Sutini; Didik Utomo Pribadi; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1503

Abstract

Sweet corn (Zea mays saccharata Sturt) cultivation can be done through variety selection technology use, fertilization, and the proper planting distance. One of the techniques used in sweet corn cultivation is planting distance and fertilization with NPK fertilizer. So, this study is critical in order to set the planting distance and NPK fertilizer dose to enhance sweet corn production. The goal of this study was to see how different planting distances and NPK fertilizer doses affected sweet corn production. The study was carried out in a rice field in Sambiroto Village, Padas District, at an elevation of ± 60 meters above sea level. It was conducted during January-May 2019. The method used was a factorial experiment with three replications based on a Randomized Block Design and two factors. The first factor is the planting distance, they are J1: 60 cm x 15 cm, J2: 60 cm x 20 cm, J3: 60 cm x 25 cm. The second factor is the NPK fertilizer dose, they are P0 = 0 kg / ha, P1 = 100 kg / ha, P2 = 200 kg / ha, P3 = 300 kg / ha. The results showed that (1) the J2 (60 x 20 cm) planting distance had the best effect on sweet corn cobs fresh weight per plant as well as per hectare without husks and sweet corn sugar content. (2) The NPK fertilizer dose of 300 kg/ha and planting distance of 60 x 20 cm resulted in optimal production on sugar content parameters. In conclusion, planting distance setting of 60 x 20 cm and NPK fertilizer dose of 300 kg / ha resulted in optimal production for sweet corn plant.
Co-Authors Agus Purwanto Alvhi Widya Calandry Alvriado Igo Fransiscus Siahaan Augustien, Nora Ayu Silviana Carolina Rosa Siallagan Didik Utomo Pribadi Djoko Agus Purwanto Felicitas Deru Dewanti Guniarti Guniarti Guniarti Guniarti Hadi Suhardjono Juli Santoso M. Rasjad Indra Milatur Royyana Muslihatin, Wirda Nora Agustien Nora Augustien Nora Augustien Pribadi, Didik Huswo Pribadi, Didik Utomo Ramadhani Mahendra Kusuma Shofiyah Zanirah Sukendah Sukendah Susilowati Susilowati Sutiman B. Sumitro Tatik W Wahyu W Widiwuriani Widiwurjani Zulfikar Alvin Naufal