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Isolasi Sinensetin dari Kumis Kucing (Orthosiphon aristatus Blume miq.) Varietas Putih Fahrauk Faramayuda; Soraya Riyanti; Adella Shindy Pratiwi; Totik Sri Mariani; Elfahmi Elfahmi; Sukrasno Sukrasno
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 6, No 2 (2021)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v6i2.48084

Abstract

Kumis kucing mengandung metabolit sekunder sinensetin yang termasuk ke dalam golongan senyawa flavonoid. Sinensetin berpotensi sebagai agen antivirus dan imunomodulator.  Tujuan penelitian ini adalah standardisasi tanaman kumis kucing varietas putih dan upaya produksi senyawa sinensetin. Ekstraksi dilakukan dengan metoda maserasi. Tahap pemisahan lanjutan dilakukan dengan metoda ekstraksi cair-cair, kromatografi kolom dan kromatografi cair vakum. Hasil ekstraksi cair-cair terpilih tiga fraksi  yaitu fraksi air, etil asetat dan n-heksana. Sebanyak 2,08 gram fraksi etil asetat dilanjutkkan pada tahap pemisahan lanjutan menggunakan kromatografi cair vakum dengan fasa diam silika gel H60 dan fasa gerak n-heksana dan etil asetat. Hasil dari kromatografi cair vakum diperoleh sebanyak 11 subfraksi. Penggabungan dilakukan pada subfraksi 8-11 yang terdeteksi adanya senyawa sinensetin, selanjutnya terhadap subfraksi gabungan dilakukan pemisaahan lanjutan dengan kromatografi kolom. Hasil pemisahan dengan kromatografi kolom diperoleh subfraksi sebanyak 142 vial. Pada subfraksi hasil kromatografi kolom nomor 91-114 terdeteksi adanya isolat sinensetin. Kromatografi lapis tipis preparatif (KLTP) gabungan subfraksi kromatografi kolom 91-114 (SFK) menunjukkan adanya senyawa sinensetin. Berdasarkan hasil pemeriksaan kemurnian dengan menggunakan KLT 2 dimensi dan analisis profil spektrum UV isolat diduga senyawa sinensetin. 
Comparison of Polyphenol Levels of Callus and Wild Type of Cat's Whiskers Plant ( Orthosiphon aristatus (Blume) Miq.) Purple Varieties Fahrauk Faramayuda; Soraya Riyanti; Ari Sri Widyaswari; Zaini Alfahmi; Sultan Salahudin Jamal; Totik Sri Mariani; Elfahmi Elfahmi; Sukrasno Sukrasno
Journal of Applied Agricultural Science and Technology Vol. 6 No. 2 (2022): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.608 KB) | DOI: 10.55043/jaast.v6i2.51

Abstract

The cat's whiskers plant ( Orthosiphon aristatus (Blume) Miq) that grows in Indonesia has white flowers, purple flowers, and white flowers with purple patterns. The quality of the secondary metabolite content of each cat's whiskers plant is different because it depends on environmental influences such as rainfall or soil mineral content. Plant tissue culture methods such as callus culture or cell culture are used to overcome these obstacles because these methods do not depend on the environment and can produce secondary metabolites such as those produced by the original plant. Cat's whiskers contain the main secondary metabolite compounds: rosmarinic acid, eupatorin, and sinensetin. Rosmarinic acid belongs to the phenolic group, while sinensetin and eupatorin are flavonoids. This study aimed to determine the total levels of polyphenols and flavonoids in purple cat whiskers and determine the ratio of secondary metabolites of cat whiskers callus with wild-type plants—determination of polyphenol content using Folin Ciocalteau reagent. Determining flavonoid levels is based on colorimetry using AlCl 3 reagent, specific for flavone and flavonol compounds. The levels of polyphenols contained in the ethanolic extract of callus and cat whiskers of the purple variety were 16.056 ± 0.204 mgQE/g and 30.780 ± 0.425 mgQE/g, while the polyphenol content of the callus ethyl acetate extract and the purple variety of cat whiskers leaves was 15,489 ± 0.425 mgQE/g and 13,262 ± 0.044 mgQE/g. The levels of flavonoids contained in the ethanol extract of the cat whiskers leaf were 1.39±0.03 mgQE/g and callus 0.16±0.02 mgQE/g in the ethyl acetate extract of the leaves of the cat whiskers 2.16±0.012 mgQE/g and callus 2.24±0.010 mgQE/g. The polyphenols and flavonoids in the callus ethyl acetate extract were more significant than the ethyl acetate extract of the purple variety ( wild type ) cat whiskers leaf.
Sosialisasi Penanganan Pasca Panen Bahan Baku Rempah Obat Tradisional Fahrauk Faramayuda; Soraya Riyanti; Akhirul Kahfi Syam; Ari Sri Windyaswari; Yeni Karlina; Remare Halomoan Limbong; Anggita Esa Putri Fitrichia; Ananda Tunjung Pertiwi; Trialisa Aprilia; Ema Mutya; Renti Mutiara Dewi; Dwi Ayu Pratiwi; Usi Septiani; Esanda Zulfi Amedea; Yesi Wulandari; Adinda Fitriani; Ami Soega Dwigantina
Kawanad : Jurnal Pengabdian kepada Masyarakat Vol. 1 No. 2 (2022): October
Publisher : Yayasan Kawanad

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56347/kjpkm.v1i2.52

Abstract

Community service activities carried out by students of Universitas Jenderal Achmad Yani were carried out on August 29, 2022, taking place in a medicinal plant garden, Faculty of Pharmacy, Universitas Jenderal Achmad Yani which was attended by UMKM Spice Nusantara Bandung participants. The socialization carried out was the presentation and delivery of post-harvest processing materials (wet sorting, washing, drying, and dry sorting) and the standardization of traditional raw materials. Conventional medicine is a form of treatment efforts in the community to support health which is currently being recommended by the government. Communities can use the toga as an economical and efficient measure to maintain body immunity because the community can obtain and use the toga quickly and simply to maintain family health. The postharvest process greatly determines the existence of active compounds that are efficacious for treatment. Incorrect postharvest management can change, reduce, or destroy the active substance of a plant into a substance that has no therapeutic effect and can even endanger health. The results of the participant's level of understanding were carried out post-test with the results of what is the purpose of post-harvest processing (100%), the sequence of post-harvest processing (80%), what is the general drying temperature (100%), a medicinal plant has good quality and efficacy then we need to know (80%), and what is the function of the washing process (90%) with the conclusion that socialization activities can significantly increase the knowledge of participants from MSMEs regarding planting, post-harvest processing, and the properties of medicinal plants significantly.