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Endang Lukitaningsih
Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
Chemical Engineering, Chemistry & Bioengineering Chemistry

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Journal : Indonesian Journal of Chemistry

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Quantification of Andrographolide Isolated from Andrographis paniculata Nees Obtained from Traditional Market in Yogyakarta Using Validated HPLC Yandi Syukri; Ronny Martien; Endang Lukitaningsih; Agung Endro Nugroho
Indonesian Journal of Chemistry Vol 16, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.901 KB) | DOI: 10.22146/ijc.21163

Abstract

This research was aimed to quantification of andrographolide isolated from A. paniculata Ness found in traditional market in Yogyakarta using validated HPLC to obtain high level content of andrographolide. The extraction of andrographolide from A. paniculata was carried out using ethanol as the solvent. Fractionation and isolation were continued using a non-polar solvent. Next, the extracts were re-crystallized to obtain isolated andrographolide. The identity of the compound was confirmed through an analysis of the melting point, IR spectra, and TLC. The purity of the compound was confirmed by the validated HPLC. The data obtained were then compared using an analytical grade of andrographolide as the standard. The isolated andrographolide confirmed melting point, IR spectra and TLC analysis were similar to the standard andrographolide. The method to determine the content of isolated andrographolide showed an adequate precision, with a relative standard deviation (RSD) smaller than 1%. The accuracy showed good recovery values were obtained for all concentrations used. The HPLC method in this study showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for the quantification of andrographolide isolated in A. paniculata. When compared to the standard, the purity of the isolated andrographolide was 95.74 ± 0.29%.
A Rapid and Simple High-Performance Liquid Chromatographic Method for Determination of Levofloxacin in Human Plasma Dion Notario; Sudibyo Martono; Zullies Ikawati; Arief Rahman Hakim; Fathul Jannah; Endang Lukitaningsih
Indonesian Journal of Chemistry Vol 17, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.483 KB) | DOI: 10.22146/ijc.23552

Abstract

A simple and rapid high-performance liquid chromatography method was developed and validated for quantifying LEV in human plasma. Chromatographic separation was performed under isocratic elution on a Luna Phenomenex® C18 (150 × 4.6 mm, 5 µm) column. The mobile phase was comprised of acetonitrile, methanol, and phosphate buffer 25 mM pH 3.0 (13:7:80 v/v/v) and pumped at a flow rate of 1.5 mL/min. Detection was performed by UV detector at a wavelength of 280 nm. Samples were pre-treated with acetonitrile followed by centrifugation, evaporation, and reconstitution step. The method proved linear (r = 0.995), sensitive (LLOQ and LOD was 1.8 and 0.6 µg/mL respectively), accurate (% error above LLOQ ≤ 12% and LLOQ ≤ 20%), precise (RSD ≤ 9%), robust in the ranges of 1.8-28.8 µg/mL, rapid (separation time not more than 10 min), and simple (use no organic additive in mobile phase). The method was showed reliable for quantifying LEV in human plasma.
Co-Authors Agung Endro Nugroho Arief Rahman Hakim Dion Notario Fathul Jannah Ronny Martien Sudibyo Martono Yandi Syukri Zullies Ikawati