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All Journal Indonesian Journal of Chemistry JURNAL ILMU KEFARMASIAN INDONESIA Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Berkala Penelitian Hayati
Ali Rohman
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences University Of Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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 1 
RAGAMAN GENETIK GEN POLIMERASE VIRUS HEPATITIS B PADA PASIEN HEPATITIS B KRONIK DENGAN PENGOBATAN TELBIVUDIN Gondo Mastutik; Juniastuti Juniastuti; Ali Rohman; Mochamad Amin; Poernomo Boedi Setiawan
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1097

Abstract

Infection caused by hepatitis B virus (HBV) is still a major global health problem and can cause liver cirrhosis and hepatocellularcarcinoma as well. Telbivudine is one among the drugs used to treat the disease routinely. However, using this drug in a long term therapymight cause mutations in HBV polymerase gene that decreases the effectiveness of the therapy. Here with the researchers report the geneticvariations of the gene isolated from telbivudine-which is used treated chronic hepatitis B patients in Surabaya, Indonesia. The blood serawere collected at Dr. Soetomo hospital from 10 telbivudine-treated and 10 untreated chronic hepatitis B patients. The DNA viral wasisolated and purified from each serum. Sequence polymerase gene at nucleotides 455 to 796 was amplified by PCR, and then analyzedbio informatically to determine their mutation profile. This study revealed a point mutation in HBV25 sample at nucleotide A1525G thatgives rise to I509V modification. Such mutation is also observed in a sequence that is available in Gen Bank with an accession numberAY641562. Additionally, the researchers found point mutations A1554G, T1593C, and C1629T in HBV25 sample and a point mutationA1554G in HBV20 sample. However, these mutations are silent. To conclude, the mutation in HBV polymerase gene among telbivudinetreatedchronic hepatitis B patients in Surabaya is known as A1525G.
HIDROLISIS BEBERAPA JENIS XILAN DENGAN ENZIM XILANOLITIK TERMOFILIK REKOMBINAN Ni Nyoman Tri Puspaningsih; Hery Suwito; Sri Sumarsih; Ali Rohman; One Asmarani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/353

Abstract

The aims of this research were to know the ability of recombinant xylanolytic enzyme from recombinant E. coli DH5a (pTP510) to hydrolyze several commercial xylan and analysis the reduction sugar product. Recombinant xylanolytic enzyme (exo-xylanase, b-xylosidase and a-L-arabinofuranosidase) could hydrolyzed several commercial xylan (oat-spelt xylan, birchwood, wheat, rye, and arabinan) with xylanolytic activities are: oat-spelt xylan (1.73 U/mL), birchwood (0.92 U/mL), wheat (6.52 U/mL), rye (4.94 U/mL), and arabinan (3.40 U/mL). Xylanolytic enzyme assay use specific substrate p-nitrophenyl-b-D-xylopyranoside (pNP-X) shown xylosidase activity 15.869 U/mL. Hydrolysis product was analyzed by HPLC. The results showed that xylose, arabinose, and xylo-oligosaccharide were produced from birchwood, wheat, rye, and arabinan hydrolysis, although xylose and arabinose were produced from hydrolysis of oat-spelt xylan.
GENOTIPE DAN SUBTIPE VIRUS HEPATITIS B PENDERITA YANG TERINFEKSI KRONIK AKTIF Gondo Mastutik; Juniastuti Juniastuti; Ali Rohman; Mochamad Amin; Poernomo Boedi Setiawan
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1077

Abstract

Chronic activivity of Hepatitis B Virus (HBV) infection can lead to liver cirrhosis or hepatocellular carcinoma. The objective of thisstudy was to know by analyzing the distribution of HBV genotypes and subtypes from hepatitis B patients suffering from chronic activehepatitis B infection in Surabaya. The HBV genotypes were determined by comparing the S gene sequences to those kept in the GeneBank. The phylogenetic tree was constructed by means of the unweighted-pair group method using arithmetic averages. Furthermore,the subtypes were deduced based on the prediction of amino acid residues 116 to 183 of HBsAg on multiple sequences alignment withClustalW2. This study involved 20 sera obtained from patients suffering chronic active hepatitis B infection. After PCR and sequencing,it was found that 13 samples could be used for sequence analysis. The results showed that all sequences were clustered into HBV genotypeB. The subtype adw2 was identified from 12 of 13 sequences, whereas one (1) belonged to ayw1. The subtype adw2 is most prevalent inIndonesia, namely in the islands of Sumatra, Java, South Kalimantan, Bali, Lombok, Ternate, and Morotai, while ayw1 is found in theislands of Nusa Tenggara and Moluccas. Based on this study, it was found that the patients with HBV subtype adw2 were from Surabaya, whereas with ayw1 was from Nusa Tenggara. It can be concluded that the HBV infected patients with chronic active hepatitis B inSurabaya have the genotype B with subtype adw2 which was originally from Surabaya, whereas, ayw1 was a patient originally fromNusa Tenggara.
Karakterisasi Glukoamilase Hasil Produksi Aspergillus niger BCS dengan Penyangga Fermentasi Sekam dan Dedak Mahyudin A. Rahman; Koesnandar Koesnandar; Gany H.; Ahmad Marasabessy; Ali Rohman; Usman Sumo FT
JURNAL ILMU KEFARMASIAN INDONESIA Vol 2 No 2 (2004): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.993 KB)

Abstract

Glucoamylase, the enzyme which converts starch to dextrins and glucose, is used in the starch-processing industry, especially for the production of glucose crystal and high fructose syrup. The aim of this work was to produce glucoamylase by Aspergillus niger BCS using rice husk as solid substrate support. For this purpose, rice husk added to the mixture was optimized on a fixed weight ratio of cassava starch and rice bran. After that, using optimum rice husk concentration, optimization of the weight ratio of cassava starch and rice bran was carried out. Glucoamylase A. niger produced in this work was characterized by determining its optimum pH and temperature, its stability under various pH and temperature, its kinetic parameters, its hydrolysis products using soluble starch as substrate, and its molecular weights. The optimum conditions for glucoamylase production by A. niger obtained when the weight ratio of cassava starch: rice bran was 1:1 (dry basis, db) and the rice husk concentration in the fermentation medium was 20% by weight (db). Under these optimized conditions, enzyme units as high as 1774 U/g dry fermented substrate was obtained when fermentation was carried out for 5 days at 30°C. Optimum pH of glucoamylase A. niger was 4.5 and its optimum temperature activity was 65°C. This enzyme was stable at pH 3.0 - 7.0 and at a temperature up to 50°C.
A THERMOPHILIC MICROBE PRODUCING DEXTRANASE FROM HEATED SUGAR CANE Afaf Baktir; Zumrotul Koiriyah; Ali Rohman
Indonesian Journal of Chemistry Vol 5, No 3 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.98 KB) | DOI: 10.22146/ijc.21794

Abstract

A thermophilic aerobe microorganism designated NP4, was isolated from the heated sugar cane. It grew on dextran, and produced a thermoactive extracellular dextranase. Screening and isolation was done by assay of dextranase activity semi quantitatively on solid medium containing blue dextran. It provided several colonies with different morphology exhibited decolourized zones around, on culture plates containing blue dextran 2000R. The screening resulted in isolation of one microbe which efficiently assimilate dextran as carbon source. Dextranase production from the choised strain in liquid medium was conducted at room temperature for 8 hours with shaking speed of 125 rpm. The dextranase enzyme showed optimum pH of 8 and optimum temperature of 60 oC.
Co-Authors AFAF BAKTIR Ahmad Marasabessy Gany H. Gondo Mastutik Hery Suwito Juniastuti Juniastuti Koesnandar Koesnandar Mahyudin A. Rahman Mochamad Amin Ni Nyoman Tri Puspaningsih One Asmarani Poernomo Boedi Setiawan Sri Sumarsih Usman Sumo FT Zumrotul Koiriyah