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Winarto Haryadi

Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta

Author-ID : 3959573

Chemical Engineering, Chemistry & Bioengineering Chemistry

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Articles

CYTOTOXICITY IDENTIFICATION OF THE SUPERNATANT OF Manihot esculenta Crantz RHIZOME AS RIBOSOME-INACTIVATING PROTEIN TO NORMAL CELL Endang Astuti; Sabirin Matsjeh; Winarto Haryadi; Deni Pranowo; Nuning Sri Mulatsih
Indonesian Journal of Chemistry Vol 4, No 2 (2004)
Publisher : Universitas Gadjah Mada

The supernatant of Manihot esculenta Crantz rhizome has been used as an alternative remedy of infectious and cancer in Jogjakarta. Cytotoxicity assay showed that Manihot esculenta Crantz supernatant had cytotoxic effect to cancer cell line, namely Myeloma (LC50 = 180,24 μg/mL) and HeLa (LC50 = 415,55 μg/mL), but have a little cytotoxic effect to SiHa. This research was aimed to identify cytotoxic activity of Manihot esculenta Crantz's supernatant to normal cell, particularly to human mononuclear cell and Vero cell line, and to compare to the cancer cell lines. The result showed that supernatant of M. esculenta had cytotoxic effect to normal mononuclear cell (LC50 = 564,00 μg/mL) and Vero cell line (LC50 = 686,00 μg/mL). The supernatant of M. esculenta had the highest cytotoxic activity to myeloma and relatively toxic to cervix cancer HeLa and normal cell, but less to SiHa.

STUDY OF EPOXIDE DECYCLISATION OF CARYOPHYLENE OXIDE WITH SYNTHETIC ZEOLITE AS CATALYSTS Winarto Haryadi; M. Muchalal
Indonesian Journal of Chemistry Vol 4, No 2 (2004)
Publisher : Universitas Gadjah Mada

The reaction of epoxide ring opening of caryophillene oxide has been done using zeolite H-Y, H-sodalit, and H-ZSM-5 as catalysts. The reactions were done in two types, there were in dioxane solvent at temperature of 110 oC and without solvent at temperature of 175 oC. The catalyst weight was 10 % from caryophillene oxide weight, and the time of reaction was four hours. The product of reaction was analyzed using GC, FTIR, and GC-MS. The reactions of caryophillene oxide in dioxane solvent with the three kinds of zeolites did not give any targeted product. Whereas, the reactions without solvent gave three main products, there was one compound with one group of secondary hidroxyl (secondary alcohol), and two compounds of ketone from caryophillene. The reaction product of caryophillene oxide obtained without using solvent with the three type of catalysts were then compared. Conversion of three main products produced by H-ZSM-5 catalyst, H-sodalit catalyst and H-Y catalyst were 82.11 %, 54.92 % and 38.53 % respectively. For that reason, the transformation of caryophillene oxide using H-ZSM-5 catalyst was considered to be the best selective product. The alcohol product was resulted from reaction between caryophillene oxide and Bronsted acid, and the ketone products was resulted from the reaction with Lewis acid in zeolite.

EXTRACTION OF PALM OIL’S FREE FATTY ACIDS BY TRIETHYLAMMINE (TEA) IN POLAR-NONPOLAR MIX-SOLVENT Endang Astuti; Winarto Haryadi; Sabirin Matsjeh
Indonesian Journal of Chemistry Vol 2, No 1 (2002)
Publisher : Universitas Gadjah Mada

Palm oil contains unsaturated fatty acids that can be oxidated and can make rancidity of the palm oil. One of the trigliserida degradation products is free fatty acid. The usage of triethylammine (TEA) in the polar-nonpolar mix solvents could enhance the free fatty acids extraction efficiency. Free fatty acids extraction from palm oil was carried out for a minute with solvents ratio: TEA 0,00-0,20M in the following solvent: 0-99% diethyl ether + 99-0% ethanol + 1% water, % volume. The extraction was also performed in the following solvent: TEA 0,00-0,20M in 0-95% heptane + 95-0% isopropanol + 5% water, % volume. Reextraction was conducted for a minute by adding Na2SO4 1M. The alcohol layer which was rich with free fatty acid was taken out and the acid value was determined. As comparison, palm oil's free fatty acids was taken out with standard method by refluxing the sample in 95% alcohol for ten minutes. Identification of the free fatty acids was done by Gas Chromatography-Mass Spectrometer. The best solvent ratio for acid value determination was TEA 0,10-0,20M in the mix-solvent: 19% diethyl ether + 80% ethanol + 1% water, % volume and the extraction product contained 8 kind of free fatty acids. In addition, TEA 0,01M in the solvent: 95% isopropanol + 5% water, % volume could be obtained 8 kind of free fatty acids too. In contrary, the free fatty acid extraction by standard method contained 7 kind of fatty acids. Linoleic acid was the free fatty acid which could not be taken out by standard method. The usage of TEA in the proper polar-non polar mix-solvent was the complete method to determine free fatty acid contained in the palm oil.

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