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Nanda Rino Nurrahmad
Mahasiswa, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Effect of Vaccination Outer Membrane Protein 52 kDa on Changes in Erythrocyte Index of Tilapia (Oreochromis niloticus) Infected by Aeromonas hydrophila Nanda Rino Nurrahmad; M. Gandul Atik Yuliani; Rahaju Ernawati; Sri Chusniati; Eduardus Bimo Aksono Herupradoto; Retno Sri Wahyuni
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28592

Abstract

The purpose of this study was to determine the effect after being vaccinated by OMP 52 kDa Aeromonas hydrophila for 1 week and then infected with Aeromonas hydrophila 10 CFU/mL for 4 days on changes in erythrocyte index. Tilapia (Oreochromis niloticus) used in this study was 10-12 cm long. There were 20 tilapia (Oreochromis niloticus) which were divided into 4 groups, consisting of 2 control groups and 2 treatment groups which were given various types of vaccine formulations. Group P0 (-) (unvaccinated and infected), Group P0 (+) (unvaccinated and infected), group P1 (vaccinated with the whole cell protein "HydroVac®" and infected), and P2 (vaccinated with Outer Membrane Protein 52 kDa and infected) by intramuscular injection. Post-treatment blood samples were collected on day 5 post-infection, collected through a caudal punctie and then analyzed using a hematology analyzer. Post-treatment outcomes led to statistically significant changes (p < 0.05). Therefore, the vaccine caused a significant change in the erythrocyte index.
Co-Authors Chusniati, Sri Eduardus Bimo Aksono Herupradoto Gandul Atik Yuliani Rahaju Ernawati Retno Sri Wahyuni