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All Journal Jurnal Medik Veteriner Journal of Parasite Science journal of Basic Medical Veterinary

Chusniati, Sri

Department Of Veterinary Microbiology - Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 3693450

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Anthelmintic Potential Extract Mango Gadung Seed (Mangifera indica L.) Mecistocirrus digitatus in vitro Ria Nikmatul Jannah; Sri Mumpuni Sosiawati; Sri Chusniati; Kusnoto Kusnoto; Rahmi Sugihartuti; Setiawati Sigit
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

This research aim to attest the potential anthelmintic extract mango seed gadung (Mangifera indica L.) against worms Mecistosirrus digitatus in vitro, the study also aims to determine the effect of immersion time, variasis concentration and the relationship between time and treatment. this study used 210 M. digitatus worm extracted regardless of gender worms. treatment that is given was the concentration extract mango seed gadung was 5%, 7.07%, 10%, 14.14%, 20%, negative control using NaCl physiological and comparison using levamisole Hidrokloride 0.0025 mL. Observations death worm Mecistocirrus digitatus and observations were made at the 6th hour, 12th hour, 18th hour and 24th hour. The results showed that variations in concentration, soaking time and the relationship between soaking time with the treatment affect mortality Mecistocirrus digitatus worms. Test results of the analysis statitistik using factorial Anova and Duncan's Multiple Range Test showed a significant difference (p<0.01) between the time factor, variations in concentration and relations between time and treatment.

Uji Total Escherichia coli pada Susu Sapi Segar di Koperasi Peternak Sapi Perah (KPSP) Karyo Ngremboko Kecamatan Purwoharjo Kabupaten Banyuwangi Adhitya Yhoga Pradika; Sri Chusniati; Muhammad Thohawi Elziyad Purnama; Mustofa Helmi Effendi; Aditya Yudhana; Prima Ayu Wibawati
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Penelitian ini bertujuan untuk mengukur kualitas susu sapi segar yang disetorkan oleh peternak ke Koperasi dan kemudian dihitung jumlah bakteri Escherichia coli yang selanjutnya dibandingkan dengan SNI. Pengambilan sampel dilakukan pada saat peternak mengantarkan susu sapi segar ke koperasi. Sampel yang diambil adalah susu yang ada di dalam milk can yang diambil dengan pipet steril dan juga karet penghisap. Sampel selanjutnya disimpan di dalam botol steril dan dibawa dengan menggunakan coolbox dilengkapi ice gel beku, selanjutnya dilakukan pengujian di Laboratorium. Selanjutnya dilakukan penanaman pada media Lauryl Tryptose Broth (LTB), Escherichia coli Broth (ECB), Levine Eosin Methylen Blue Agar (L-EMBA) dan dilakukan uji biokimia Indole, Methyl Red, Voges Proskauer, Citrate (IMViC) selanjutnya dilakukan pendugaan jumlah bakteri dengan menggunakan Most Probable Number (MPN). Hasil penelitian didapatkan bakteri Escherichia coli yang melebihi standart SNI > 3 APM/g yaitu 5 dari 16 sampel atau 31,25%. Hasil perhitungan berdasarkan MPN, sampel 7 menghasilkan angka 3APM/g, sampel 9 menghasilkan angka 3,6 APM/g, sampel 10 menghasilkan angka 23 APM/g, sampel 13 menghasilkan angka 7,4 APM/g dan sampel 14 menghasilkan angka 3,6 APM/g.

Isolasi dan Identifikasi Staphylococcus aureus pada Susu Kambing Peranakan Etawah Penderita Mastitis Subklinis di Kelurahan Kalipuro, Banyuwangi Laila Nur Hayati; Wiwiek Tyasningsih; Ratih Novita Praja; Sri Chusniati; Maya Nurwartanti Yunita; Prima Ayu Wibawati
Jurnal Medik Veteriner Vol. 2 No. 2 (2019): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Staphylococcus aureus merupakan salah satu bakteri yang sering menyebabkan mastitis subklinis pada Kambing Peranakan Etawah (PE) yang menimbulkan kerugian ekonomi yang cukup besar akibat turunnya produksi susu. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Staphylococcus aureus pada susu kambing PE pederita mastitis subklinis di Kelurahan Kalipuro, Banyuwangi. Sampel yang digunakan berupa susu kambing PE yang diambil pada bulan Desember 2018 di Kelurahan Kalipuro, Banyuwangi. Metode sampling yang digunakan yaitu metode purposive sampling dimana kriteria kandang yang menggunakan bahan baku kayu dan bambu, umur kambing PE 2-3 tahun, kambing yang aktif diperah dan terbukti positif 3 dan 4 mastitis subklinis melalui uji CMT. Sampel yag didapat sebanyak 17 kemudian dianalisis laboratorik di laboratorium mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga Banyuwangi. Tahap isolasi pada media MSA dan dilanjutkan tahap identifikasi dengan menggunakan pewarnaan Gram, uji katalase, uji manitol, uji koagulase dan uji VP. Diperoleh hasil yaitu terdapat 16 (94.1%) sampel positif Staphylococcus aureus.

Isolasi dan Identifikasi Streptococcus sp. dari Sapi Perah Penderita Mastitis Subklinis di Purwoharjo Banyuwangi Adinda Dilla Pribadi; Aditya Yudhana; Sri Chusniati
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Mastitis subklinis adalah radang pada ambing yang tidak menunjukkan gejala klinis dan dapat menurunkan produksi susu. Streptococcus sp. adalah salah satu mikroorganisme patogen yang dapat menyebabkan mastitis subklinis pada sapi perah. Tujuan dari penelitian ini adalah untuk mendeteksi Streptococcus sp. pada susu sapi perah. Sebanyak 26 ekor sampel sapi perah dilakukan deteksi mastitis subklinis menggunakan Uji California Mastitis Test (CMT) di Purwoharjo, Banyuwangi. Isolasi dan identifikasi bakteri dilakukan dengan menggunakan kultur agar, pewarnaan Gram, dan uji biokimia. Hasil penelitian menunjukkan bahwa bakteri Streptococcus sp. terdeteksi pada 5 (19.23%) ekor sampel di Purwoharjo Banyuwangi.

Carcass Quality and Tetracycline Residues in Broiler Chicken Meat in Banyuwangi Traditional Market Safira Fauziyyah Subagyo; Nenny Harijani; Prima Ayu Wibawati; Mustofa Helmi Effendi; Sri Chusniati; Maya Nurwartanti Yunita
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.1-7

This study aimed to examine the quality of broiler carcasses in the Banyuwangi traditional market with carcass quality examination based on SNI number 3924-2009 and tetracycline antibiotic residues test based on SNI number 7424:2008. A total of ten broiler chicken sellers as representative of almost all broiler chicken sellers in the Banyuwangi market, with each seller was taken for one carcass in this research. Carcass quality examination used a standart carcasses weight 1,0-1,3 kg. Carcass quality examination was carried out with descriptive analysis with reference to the physical characteristics of SNI (Standarisasi Nasional Indonesia). The bioassay method is used to test for tetracycline antibiotic residue. The result of the carcass quality examination showed that from the whole sample consisting of conformation, disjointed or broken bones and missing parts, discolorations, and cleanliness of broiler carcasses are good enough. The percentage quality of broiler carcasses quality I is 60%, quality II is 30% and quality III is 10%. The examination of antibiotic tetracycline residues in broiler meat showed negative results indicating that no feeds containing additional antibiotics made carcasses in the traditional Banyuwangi market safe for consumption.

PEMBERIAN EKSTRAK MENIRAN (Phyllanthus niruri Linn) TERHADAP GAMBARAN HISTOPATOLOGI INFUNDIBULUM AYAM PETELUR YANG DIINFEKSI Escherichia coli Adinda Riezki Irawan; Emy Koestanti Sabdoningrum; Sri Hidanah; Sri Chusniati; Sri Pantja Madyawati; Benjamin Christoffel Tehupuring
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19992

The purpose of this research aimed to prove the effect of meniran extract (Phyllanthus niruri Linn) on layer hen infected with Escherichia coli to reduce inflammation and congestion on infundibulum histopathological view. This research used 20 samples of layer hen. The treatment in this research was P0- (not infected with Esherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P0+ (infected with Escherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P1 (infected with Escherichia coli and treated with 10% meniran extract (Phyllanthus niruri Linn)), P2 (infected with Escherichia coli and treated with 20% meniran extract (Phyllanthus niruri Linn)), P3 (infected with Escherichia coli and treated with 30% meniran extract (Phyllanthus niruri Linn)). The data of this study analyzed by Kruskal-wallis and followed with Mann-withney test. The result of this research can be concluded that 10% concentration of meniran extract (Phyllanthus niruri Linn) was effective to eliminate Escherichia coli, 20% concentration of meniran extract (Phyllanthus niruri Linn) able to promote antibacterial activity, and 30% concentration of meniran extract shown antibacterial rate on high potential so it can be used to reduce inflammation and congestion as the result of Escherichia coli infection.

Effect of Vaccination Outer Membrane Protein 52 kDa on Changes in Erythrocyte Index of Tilapia (Oreochromis niloticus) Infected by Aeromonas hydrophila Nanda Rino Nurrahmad; M. Gandul Atik Yuliani; Rahaju Ernawati; Sri Chusniati; Eduardus Bimo Aksono Herupradoto; Retno Sri Wahyuni
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28592

The purpose of this study was to determine the effect after being vaccinated by OMP 52 kDa Aeromonas hydrophila for 1 week and then infected with Aeromonas hydrophila 10 CFU/mL for 4 days on changes in erythrocyte index. Tilapia (Oreochromis niloticus) used in this study was 10-12 cm long. There were 20 tilapia (Oreochromis niloticus) which were divided into 4 groups, consisting of 2 control groups and 2 treatment groups which were given various types of vaccine formulations. Group P0 (-) (unvaccinated and infected), Group P0 (+) (unvaccinated and infected), group P1 (vaccinated with the whole cell protein "HydroVac®" and infected), and P2 (vaccinated with Outer Membrane Protein 52 kDa and infected) by intramuscular injection. Post-treatment blood samples were collected on day 5 post-infection, collected through a caudal punctie and then analyzed using a hematology analyzer. Post-treatment outcomes led to statistically significant changes (p < 0.05). Therefore, the vaccine caused a significant change in the erythrocyte index.

The Efficacy of Putrimalu Root Extract (Mimosa pudica) Against Mortality and Histopathological of Rat Muscles Injected Naja sputatrix Venom Briantono Willy Rendragraha; Djoko Legowo; Suryo Kuncorojakti; Sri Chusniati; Arimbi Arimbi
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36486

The purpose of this experiment was to know the effect of Mimosa pudica root extract (normal water extraction) on mortality and muscle histopatological in experimental animal (Rattus norvegicus) which injected with LD50 of Naja sputatrix venom. Fresh Mimosa pudica root dried without sunshine and ground into coarse powder with steamroller (mash 3mm). Plant extract were prepared by stirring 4 g of the powder into 200 ml of water for 3 hours at room temperature and filtered with muslin cloth before freezdried. 30 rat divided into five groups, P0(C+), P1(C-) P2, P3, and P4. P0 (positive control group) injected by saline intra muscular and given aquadest peroral after five minutes, P1 (negative control) injected with Naja sputatrix venom and given aquadest peroral after five minutes. P2, P3, and P4 injected by Naja sputatrix venom and given Mimosa pudica extract with 250mg/KgBW, 500mg/KgBW, and 1000mg/KgBW doses. All of the experimental animal observed about six hours and than euthanized, musculus gluteus maximus (location of injection) was taken for histopatological examination. In this experiment observed that the normal water extraction of mimosa pudica can’t reduce mortality of experimental animals but it can decrease muscle damage in 1000mg/KgBW dose.

Co-Authors Adhitya Yhoga Pradika Adinda Dilla Pribadi Adinda Riezki Irawan Aditya Yudhana Arimbi Arimbi Benjamin Christoffel Tehupuring Briantono Willy Rendragraha Djoko Legowo Eduardus Bimo Aksono Herupradoto Emy Koestanti Sabdoningrum Gandul Atik Yuliani Kusnoto Kusnoto Laila Nur Hayati Maya Nurwartanti Yunita Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nanda Rino Nurrahmad Nenny Harijani Prima Ayu Wibawati Rahaju Ernawati Rahmi Sugihartuti Ratih Novita Praja Retno Sri Wahyuni Ria Nikmatul Jannah Safira Fauziyyah Subagyo Setiawati Sigit Sri Hidanah Sri Mumpuni Sosiawati Sri Pantja Madyawati Suryo Kuncorojakti Tyasningsih, Wiwiek

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