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All Journal Indonesian Journal of Urology Journal of Mathematical and Fundamental Sciences JURNAL BIOLOGI INDONESIA The Indonesian Biomedical Journal
Marselina Tan
School of Biological technology and science. Bandung Technological Institute, Bandung.
Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Mathematics Medicine & Pharmacology Physics Public Health

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PENGARUH ESTRADIOL-17? DAN KOLAGEN TIPE IV TERHADAP EKSPRESI GEN PIK3CA UNTUK MENGINDUKSI EKSPRESI C-ERBB2 PADA LINI SEL KANKER OVARIUM SKOV-3 Martgrita, Merry Meryam; Tan, Marselina Irasonia
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2312

Abstract

Ovarian cancer cell metastasis is induced by signaling pathway activated by the binding of type IV collagen to ?1 integrinreceptor on the surface of cancer cells and estrogen binding to estrogen receptor. However, the role of estradiol-17? andtype IV collagen on the development of ovarian cancer have not been clearly understood. Therefore, this research wasconducted to observe the differential gene expression in SKOV-3 ovarian cancer cells cultured on type IV collagen andtreated with estradiol-17?, and incubated for 12, 24 and 72 hours. Differential display RT-PCR was used to express thedifferential expression gene after treatment. cDNA fragment that expressed differentially was isolated and sequenced.Sequencing result on one of the cDNA fragment showed that PI3K is one of the gene expressed in SKOV-3 ovarian cancercell. To verify this result, cDNA was amplified using PIK3CA specific primer. The increasing level of PIK3CA is inducedby three kinds of receptor activities, those are c-erbB2 receptor bound to estradiol-17?, homodimer receptor of c-erbB2, andthe activity of integrin receptor bound to type IV collagen. The increasing level and activity of PIK3CA can also increasethe expression of c-erbB2 gene. In SKOV-3 cells cultured on type IV collagen for 72 hours, the increasing of PIK3CA andc-erbB2 expression level is very low. The conclusion is that estradiol-17? gives a more significant effect than type IVcollagen to induce the increasing expression of PIK3CA and c-erbB2 genes.Keywords: ovarian cancer, SKOV-3, type IV collagen, estradiol-17?, PIK3CA, c-erbB2
EFFECT OF TESTICULAR TORSION ON SPERMATOZOA IN CONTRALATERAL EPIDIDYMIS Pramod, Sawkar Vijay; Sugandi, Suwandi; Sihombing, Aaron Tigor; Tan, Marselina
Indonesian Journal of Urology Vol 19 No 2 (2012)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v19i2.62

Abstract

Objective: To determine the abnormality of spermatozoa in the contralateral epididymis after unilateral testicular torsion. Material & method: Twenty wistar rats were divided into two groups i.e. Group B (sham procedure) Group A (torsio and orchiectomy 24 hours later), and contralateral epididymectomy was performed a month later. Spermatozoa in the contralateral epididymis are extracted and analyzed by an experienced biologist. Data were analyzed using Chi-square or Fischer exact test. Results: Sperm morphology changes in group B is higher than Group A (6,6% vs 0,5%, p = 0,009). Conclusion: Unilateral testicular torsion causes sperm abnormal morphology in the contralateral epididymis. Keywords: Unilateral testicular torsion, contralateral epididimal spermatozoa.
Inhibition of Mammary Gland Cancer Development by Propolis and Mangostin in Female Mice Balb/C Marselina Irasonia Tan; Irham Hayati
Journal of Mathematical and Fundamental Sciences Vol. 49 No. 1 (2017)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2017.49.1.4

Abstract

The development of breast cancer involves many processes, including angiogenesis and metastasis. Some factors play a major role in angiogenesis, such as HIF-1α, and in metastasis, such as FAK and Wnt2. The aim of this study was to observe the effect of propolis and mangostin on the development of mammary gland cancer and on the expression of Wnt2 and FAK in Balb/C mice. Mammary gland tumors were induced in Balb/C mice by DMBA. The mice were divided into 5 treatment groups: negative control (K"‘); positive control treated with doxorubicin (14.04 mg/kg bw) (Dx); mice treated with propolis (0.32 mg/kg bw) (P) or mangostin (0.128 mg/kg bw) (M); and mice treated with a combination of propolis (0.32 mg/kg bw) and mangostin (0.128 mg/kg bw) (MP). Both mangostin and propolis did not affect the body weight of the mice. Treatment with propolis or treatment with propolis combined with mangostin was able to reduce tumor development activity in Balb/C mice. Moreover, the combination of mangostin and propolis was able to lower Wnt2, FAK and HIF-1α expression. It can be concluded that the combination of propolis and mangostin has potential to inhibit cancer development through downregulation of Wnt2, FAK, and HIF1α expression.
Role of Hypoxia on Growth and Differentiation of Human Adipose Derived Stem Cells Grown on Silk Fibroin Scaffold Induced by Platelet Rich Plasma Anggraini Barlian; Marselina Irasonia Tan; Ergha Widya Sarjana; Noviana Vanawati
Journal of Mathematical and Fundamental Sciences Vol. 53 No. 3 (2021)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2021.53.3.6

Abstract

Previous research has proven that 10% platelet-rich plasma (PRP) can enhance growth and differentiation of human adipose derived stem cells (hADSC) grown on silk fibroin scaffold into chondrocytes. A low oxygen concentration (hypoxia) condition is an important factor that potentially affects the ability of hADSC to grow and differentiate. The objective of this research was to analyze the difference in growth and differentiation capacity of hADSC grown on salt leached silk fibroin scaffold supplemented by 10% PRP under normoxic and hypoxic conditions. The growth capacity of the hADSC was determined by MTT assay and differentiation was tested using glycosaminoglycan (GAG) content analysis, while chondrocyte markers were visualized with the immunocytochemistry (ICC) method. This research observed hADSC proliferation under normoxic and hypoxic conditions for 21 days. Visualization of type 2 collagen showed that it was more abundant under hypoxia compared to normoxia.  HIF-1α was only detected in the hADSC cultured in hypoxic conditions. In conclusion, culture under hypoxic conditions increases the capacity of hADSC to grow and differentiate into chondrocytes. This is the first study that has shown that hypoxia is able to enhance the proliferation and differentiation of hADSC grown on 3D salt leached silk fibroin scaffold supplemented by 10% PRP.
SARS-CoV-2 Neutralization Assay System using Pseudo-lentivirus Anastasia Armimi; Afina Firdaus Syuaib; Katherine Vanya; Marselina Irasonia Tan; Dessy Natalia; David Virya Chen; Chikako Ono; Yoshiharu Matsuura; Anita Artarini; Ernawati Arifin Giri-Rachman
The Indonesian Biomedical Journal Vol 15, No 2 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i2.2212

Abstract

BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects humans' lower respiratory tracts and causes coronavirus disease-2019 (COVID-19). Neutralizing antibodies is one of the adaptive immune system responses that can reduce SARS-CoV-2 infection. This study aimed to develop a SARS-CoV-2 neutralization assay system using pseudo-lentivirus.METHODS: The plasmid used for pseudo-lentivirus production was characterized using restriction analysis. The gene encoding for SARS-CoV-2 spike protein was confirmed using sequencing. The transfection pseudo-lentivirus optimal condition was determined by choosing the transfection reagents and adding centrifugation step. Optimal pseudo-lentivirus infection was analysed using fluorescent assay and luciferase assay. The optimal condition of pseudo-lentivirus infection was determined by the target cell type and the number of pseudo-lentiviruses used for neutralization test. SARS-CoV-2 pseudo-lentivirus was used to detect neutralizing antibodies from serum samples.RESULTS: The plasmid used for pseudo-lentivirus production was characterized and confirmed to have no mutations. Lipofectamine 2000 reagent generated pseudo-lentivirus with a higher ability to infect target cells, as indicated by a percentage green fluorescent protein (GFP) of 12.68%. Pseudo-lentivirus centrifuged obtained more stable results in luciferase expression. Optimal pseudo-lentivirus infection conditions were obtained using puromycin-selected HEK 293T-ACE2 cells as target cells. The number of pseudo-lentiviruses used in the neutralization assay system was multiplicity of infection (MOI) 0.075. Serum A samples with a 1:10 dilution had the highest neutralizing antibody activity.CONCLUSION: This study shows that SARS-CoV-2 neutralization assay system using pseudo-lentivirus successfully detected neutralizing antibodies in human serum, which were indicated by a decrease in the percentage of pseudo-lentivirus infections.KEYWORDS: COVID-19, neutralizing antibody, neutralization assay, pseudo-lentivirus, SARS-COV-2
Co-Authors Aaron Tigor Sihombing Afina Firdaus Syuaib Anastasia Armimi Anggraini Barlian Anita Artarini Chikako Ono David Virya Chen Dessy Natalia Ergha Widya Sarjana Ernawati Arifin Giri-Rachman Irham Hayati Katherine Vanya Martgrita, Merry Meryam Noviana Vanawati Sawkar Vijay Pramod Suwandi Sugandi Yoshiharu Matsuura