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All Journal Dinamisia: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education
Andreas Binar Aji Sukmana
Fakultas Biologi Universitas Kristen Satya Wacana
Agriculture, Biological Sciences & Forestry Social Sciences Other

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Characterization of Ficus benjamina and Artocarpus heterophyllus Proteases as Potential Rennet Alternatives Sukmana, Andreas Binar Aji; Widyaningrum, Indy; Lani, Rischa Karmila; Kasmiyati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23516

Abstract

Rennet, a milk coagulant exhibiting proteolytic activity, is a crucial component in cheese industries. Its price and availability have discouraged the growth of some small scale cheese industries. Therefore, an alternative for rennet will be beneficial for the industries. Among other sources, plant proteases offer some advantages as rennet alternatives. This study aimed to investigate the potential of plant proteases obtained from the latex as potential rennet alternatives. A total of six plants from the genus Ficus and Artocarpus were screened for their proteolytic activity and milk coagulating ability. The screening indicated that all six tested plants displayed proteolytic activity at various levels, but only Ficus benjamina and Artocarpus heterophyllus produced a firm milk curd. Hence, both F. benjamina and A. heterophyllus were determined to be the most potential. Further characterizations suggested that F. benjamina and A. heterophyllus protease were optimum at pH 7.0 also at 50°C and 40°C, respectively. At their optimum conditions, both proteases exhibited a lower MCA/PA ratio than that of the rennet. This study contributed to scientific knowledge development by becoming the first to characterize the optimum conditions of F. benjamina and A. heterophyllus’ proteases, investigate their MCA/PA ratio, and compare their activity against commercial rennet. The examination of their potentials as rennet alternatives could benefit small cheese industries and the communities.
Antibacterial Activity and Flavonoids Content of Artemisia cina Berg. ex Poljakov Ethyl Acetate Extracts Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok; Herawati, Maria Marina; Sukmana, Andreas Binar Aji
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27665

Abstract

The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial  potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent. 
The Pendidikan Lingkungan Hidup sebagai Upaya Konservasi di Kawasan Wisata Gedong Songo Desti Cahyaningrum; Andreas Binar Aji Sukmana; Rully Adi Nugroho
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2021): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v5i1.4153

Abstract

Gedong Songo is a National Tourism Development Area in Bandungan region with an increasing number of visitors. Previous studies found that littering and water pollution were some of the prominent problems in Gedong Songo. The lack of knowledge and awareness towards environmental management was identified as the center of the issues. A conservation effort in the form of an early environmental education targeted elementary school students was suggested to address this problem. The environmental education was performed in State Elementary School Candi 1 and Candi 3 Bandungan and was designed as a series of activities, including lectures, discussions, observations, as well as experimental activities. As a result, many students were found to be actively engaged throughout the activities and showed an improved attitude. They realized the importance of washing hands, keeping environmental hygiene, and problem-solving. Further evaluation indicated that the students had developed a better understanding of general environmental management. Therefore, it can be concluded that early environmental education can potentially transform students' knowledge and awareness about the importance of environmental conservation. Through these activities, it is expected that the obtained experiences will support the Gedong Songo environmental conservation in the future.
Co-Authors Desti Cahyaningrum Elizabeth Betty Elok Kristiani Lani, Rischa Karmila Maria Marina Herawati Rully Adi Nugroho Sri Kasmiyati Widyaningrum, Indy Yayi Suryo Prabandari