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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL ILMU PERTANIAN AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Karet Jurnal Ilmu Pangan dan Hasil Pertanian Jurnal Penelitian Pertanian Terapan Biosaintifika: Journal of Biology & Biology Education Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Ilmu Ilmu Agribisnis: Journal of Agribusiness Science Jurnal Triton Wahana Peternakan Jurnal Akademika Kimia Magistrorum et Scholarium: Jurnal Pengabdian Masyarakat Teknologi dan Industri Pertanian Indonesia National Multidisciplinary Sciences AGRIC Open Access DRIVERset
Maria Marina Herawati
Univeristas Kristen Satya Wacana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Public Health Social Sciences Veterinary Other

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OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE Herawati, Maria Marina; Pudjihartati, Endang; Pramono, Suwijiyo; Sulistyaningsih, Endang; Purwantoro, Aziz
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.Keywords: 2,4-D, Artemisia cina, BA, polyploidy
DAYA SAING BUDIDAYA AYAM RAS PEDAGING PADA BERBAGAI POLA USAHA Herawati, Maria; Haryono, Dwi; Lestari, Dyah Aring Hepiana
Jurnal Ilmu-Ilmu Agribisnis Vol 4, No 3 (2016)
Publisher : Lampung University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.061 KB) | DOI: 10.23960/jiia.v4i3.%p

Abstract

The purpose for this research is to know the implementation partnership in broiler chicken farming with contract agreement and verbal agreement, to analyze the competitiveness broiler chicken farming and to analyze sensitivity of competitiveness due to changes in price of input and output.  The data was analyzed by descriptive qualitative using PAM (Policy Analysis Matrix) analysis model and elasticity.  The result showed that private profit of autonomous pattern was Rp2,582,549 and social profit was Rp2,740,642 per 1,000 birds. Private profit of partnership pattern with contract agreement was Rp707,381 and social profit was Rp3,898.950.  Private profit of partnership pattern with verbal agreement was Rp845,200 and social profit was Rp1,491,519.  Based on the analysis competitiveness, it showed that broiler chicken farm in South Lampung Regency had competitive and comparative advantages, in which PCR and DRCR value 0.38; 0,37 (autonomous pattern), 0.75; 0,35 (contract agreement pattern) and 0.69;0,56 (verbal agreement pattern). Elasticity value showed that competitiveness of broiler chicken farming in South Lampung Regency was sensitive with the price of broiler chicken and also price of broiler feed.Key words: Broiler chicken, competitiveness, PAM (Policy Analysis Matrix)
KOMPOSISI EKSTRAK STEVIA (STEVIA REBAUDIANA) TERHADAP KARAKTERISTIK SIRUP BIT (BETA VULGARIS L.) Simarmata, Elvi Fitriani; Herawati, Maria Marina; Sutrisno, Alfred Jansen; Handoko, Yoga Aji
Jurnal Penelitian Pertanian Terapan Vol 19, No 3 (2019)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v19i3.1429

Abstract

Beetroots are a type of tubers with a distinctive purplish red color and are beneficial for health with a high amount of betasianin as an antioxidant, as well as its use in preventing infection. But the beetroot is less liked by the public because of the strong enough earthy taste, so it is necessary to do new innovations for the product to be accepted by the community, one of which is the functional beet syrup drink. To create a preferred flavor, the addition of other food additives and sweetener stevia which has a sweetness level of 200-300 times that of sugar and includes non-calorie sweeteners is done. This study aims to analyze the best concentration of stevia extract on the sweetness level and beetroot characteristics and determine the level of panelists' preference for beetroot syrup (Beta vulgaris L.) with organoleptic tests. This research was conducted with a randomized block design (RBD), the data were analyzed by variance (ANOVA), if the data obtained were significantly different, the DMRT would be further tested at 5% level. The results showed that the highest betasianin was in the SBS15 treatment (31.74 mg / l), the highest total dissolved solids was in the SBS treatment (3.25ºBrix), the highest pH was in the SBS15 treatment (4.22), the highest vitamin C was in the SBS15 treatment (11.66 mg ascorbic acid / 10 ml). Organoleptic results showed that beet syrup with the addition of stevia extract of 6% was the most preferred sample by panelists. 
PERTUMBUHAN ARTEMISIA VULGARIS SECARA KULTUR PUCUK PADA MEDIUM DENGAN KANDUNGAN MIOINOSITOL DAN EKSTRAK KHAMIR Kasmiyati, Sri; Herawati, Maria M.; Kristiani, Elizabeth B.E.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.038 KB) | DOI: 10.24002/biota.v13i2.2672

Abstract

The effects of mioinositol and yeast extract were studied to assess their influence on growth of plantlets of Artemisia vulgaris by shoot culture. The plants regeneration of A. vulgaris were established by removing the nodes of stem and growing in MS multiplication medium with 1 ppm kinetin and 1 ppm NAA for 4 weeks. Shoots were induced for roots on MS treatment medium supplemented with mioinositol and yeast extract, added with 2 ppm IBA. Combination of four levels mioinositol concentration (mg/l): 100, 200, 300, and 400, and four levels of yeast extract concentration (mg/l): 0, 200, 300, and 400 were simultaneously added. Plantlets (2 weeks) were sub cultured on semi liquid MS medium. Plantlets were harvested on 6 weeks old. Measured parameters were fresh weight of plantlets. Data were analyzed using ANOVA followed by HSD test (p=95%). The results showed that the treatment of mioinositol and yeast extract were not significantly influenced on fresh weight of plantlets. Yeast extract was not influenced the growth of plantlets. The growth and morphogenesis of plantlets A. vulgaris were induced in treatment 100 ppm mioinositol, and addition mioinositol were higher than 100 ppm not significantly influenced the growth of plantlets.
EFFECT OF INDUCED POLYPLOIDY ON PLANT GROWTH, CHLOROPHYLL AND FLAVONOID CONTENT OF ARTEMISIA CINA Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok; Herawati, Maria Marina
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.361 KB) | DOI: 10.15294/biosaintifika.v12i1.22548

Abstract

Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.
Embriogenesis Somatik dari Eksplan Benih Gandum Tropis (Triticum aestivum L.) Pudjihartati , Endang; Marina Herawati , Maria
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 2 (2012): June 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (24.699 KB) | DOI: 10.24002/biota.v17i2.136

Abstract

Embriogenesis somatik dalam penelitian ini menggunakan eksplan embrio dewasa (benih) gandum tropis varietas Dewata dan galur R-036 dan R-040 koleksi Pusat Studi Gandum Fakultas Pertanian dan Bisnis UKSW, meliputi tahap induksi dan proliferasi. Penelitian ini bertujuan mendapatkan genotipe gandum tropis yang mudah di kultur secara in vitro dan medium yang baik dalam rangka memperoleh Embrio Somatik (ES). Pada tahap induksi, benih gandum dikultur pada empat konsentrasi 2,4-d (2; 2,5; 3 dan 3,5 ppm). Pada tahap proliferasi diteliti empat komposisi media yang mengandung 2,5 dan 3 ppm 2,4-d dengan dan tanpa 0,5 ppm BAP. Hasil penelitian menunjukkan bahwa jumlah ES dan skor ES primer relatif tertinggi dari varietas Dewata pada 3 ppm 2,4-d, galur R-040 pada 2,5 ppm 2,4-d, dan galur R-036 pada 2 ppm 2,4-d. Eksplan gandum galur R-036 menghasilkan jumlah ES dan skor ES primer relatif tertinggi dibandingkan genotipe lain. Mutu benih yang digunakan mempengaruhi respons eksplan dalam menghasilkan jumlah ES dan skor ES primer dan kurang mempengaruhi jumlah ES dan skor ES sekunder. Pada tahap proliferasi, jumlah ES sekunder relatif tertinggi, dari galur R-036 pada 2,5 ppm 2,4-d, sedangkan varietas Dewata dan R-040 berturut-turut pada 2,5 ppm dan 3,0 ppm 2,4-d. Aplikasi 2,4-d yang dikombinasikan dengan BAP dapat menurunkan jumlah ES, tetapi hanya cenderung menurunkan skor ES sekunder pada ketiga genotipe. Pada skor ES hasil proliferasi tidak diamati adanya pengaruh komposisi ZPT yang diteliti (2,4-d dan BAP) pada varietas Dewata dan galur R-040, sedangkan pada galur R-036 skor ES sekunder maksimum pada 2,5 ppm 2,4-d.
Efisiensi Ekonomi Pemeliharaan Ayam Kampung Super yang Diberi Fitobiotik dengan Teknologi Nanoenkapsulasi Minyak Buah Merah (Pandanus conoideus) Maria Herawati; Bangkit Lutfiaji Syaefullah; Ni Putu Vidia Tiara Timur
Wahana Peternakan Vol. 4 No. 2 (2020): Volume 4 Nomor 2 September 2020
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v4i2.209

Abstract

Red fruid as one of Indonesia’s native medicinal plants as a phytobiotic is developed to maintain the health status of livestock. The drawback of using phytobiotics as feed additives is done by making nanoencapsulation so that the products are easily absorbed by the livestock digestive system. This research was conducted to know the value of the economic efficiency using red fruit oil nanoencapsulation in super native chickens. The study used a descriptive method with 5 treatments and 4 replication. P0 (control), P1 (2.5% red fruit oil extract), P2 (2.5% red fruit oil nanoencapsulation), P3 (5% red fruit oil nanoencapsulation), P4 (10% red fruit oil nanoencapsulation). The result showed that the economic efficiency of using feed in each treatment was efficient (EEPP<1) dan the best business efficiency (R/C) in P2 treatment (1,474). The conclusion of this study is that the use of red fruit oil nanoencapsulation in super native chicken can provide a better economic efficiency value than those without nanoencapsulation. The use of 2.5% red fruit oil nanoencapsulation gave the best economic efficiency value compared to other treatments.
Antibacterial Activity and Flavonoids Content of Artemisia cina Berg. ex Poljakov Ethyl Acetate Extracts Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok; Herawati, Maria Marina; Sukmana, Andreas Binar Aji
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27665

Abstract

The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial  potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent. 
Production of Sugar From Sweet Sorghum Stems with Hydrolysis Method Using Trichoderma viride Ndapamuri, Melycorianda Hubi; Herawati, Maria Marina; Meitiniarti, V Irene
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.25954

Abstract

Sorghum stem bagasse waste is one of the materials with high cellulose content. It can be utilized in glucose production through enzymatic hydrolysis of cellulose by Trichoderma viride. This study aims to determine sorghum stem bagasse’s potential in producing glucose, assessing the time and concentration of sorghum stem bagasse in the hydrolysis process to produce glucose optimally and following SNI. Hydrolysis was carried out using a concentration of 5%, 8%, and 11% sorghum stem bagasse for 10, 15, and 20 days. The results showed that sorghum stem bagasse waste could produce glucose with an average glucose yield of 10.09% to 24.40 %. There is a tendency that increasing substrate concentration and hydrolysis time will increase the yield of liquid glucose. The treatment of 5% concentration of sorghum stem bagasse with a long hydrolysis time of 10 days can produce the highest liquid glucose, namely 24.40% with total dissolved solids of 7.40% Brix, the ash content of 0.26%, but 47.54% water content has not met SNI standards. 
Pengaruh Konsentrasi Minyak Nabati terhadap Lama Simpan dan Kualitas Pasta Bawang Merah (Allium ascalonicum L.) Ndruru, Celvia Carlinawati; Herawati, Maria Marina
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 1 (2021): February 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i1.2929

Abstract

Shallots (Allium ascalonicum L.) have a short shelf life because these tubers can experience decay and premature germination. Therefore, innovation is needed in shallot processing, one of which is by processing shallots into pasta products. This research conducted the making of onion paste with the addition of vegetable oil to improve the quality of the paste. The purpose of this study was to examine the effect of vegetable oil concentration treatment on shelf life and quality of shallot paste and to determine the level of preference for the panelists to shallot paste by organoleptic test. This study used a completely randomized design (CRD), namely one treatment factor with the addition of vegetable oil concentrations of 30%, 25%, and 20%. The data were analyzed using variance, if the results were significantly different, then it was further tested with DMRT with a significant level of 5%. The results showed that the concentration of vegetable oil on shelf life and quality of shallot paste were not significantly different so that the addition of vegetable oil concentration had no effect on shelf life and quality of shallot paste. Panelists preferred color and taste parameters of shallot paste with the addition of 30% vegetable oil. Meanwhile, for the pasta aroma that the panelists like, the paste with the addition of 25% and 20% vegetable oil.
Co-Authors Adella Natasha Andre Agusta Andreas Binar Aji Sukmana Andreas Binar Aji Sukmana Andree Wijaya Setiawan Ayu Valentina Ayu Valentina Aziz Purwantoro Aziz Purwantoro Bangkit Lutfiaji Syaefullah Bayu Setyaji Chrismita B. Lusihanne Clement Panji Dinda Sukma Wibowo Dwi Haryono Dyan Yulianti Ebit Eko Bachtiar Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Elizabeth Betty Elok Kristiani Elizabeth Betty Elok Kristiani Endang Pudjihartati Endang Pudjihartati Endang Sulistyaningsih Farid Maulana Fransisca Mareta Kurnia Sari Hendrik Johannes Nadapdap Herry Susanto Ivan Tjahja Pranata Jennifer Larisa Liem Lestari, Dyah Aring Hepiana Meitiniarti, V Irene Melycorianda H. Ndapamuri Naning Restiani Titaley Ndruru, Celvia Carlinawati Ni Putu Vidia Tiara Timur Ni Putu Vidia Tiara Timur Rastosari A Sari Setiyowati Simarmata, Elvi Fitriani Sri Kasmiyati Suwijiyo Pramono Suwijiyo Pramono Theresa Dwi Kurnia Tinjung Mary Prihtanti Yayi Suryo Prabandari Yoga Aji Handoko Yohanes Hendro Agus Yohanes Hendro Agus