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All Journal Jurnal Penelitian Saintek Bioma : Berkala Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Eksergi Journal of Natural Sciences and Mathematics Research Jurnal Kimia Riset Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education JKPK (Jurnal Kimia dan Pendidikan Kimia) International Journal of Active Learning Jurnal Ilmiah Sains Gunung Djati Conference Series
Elizabeth Betty Elok Kristiani
Universitas Kristen Satya Wacana
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Other

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Quality of Yeast Extract of Industry Yeast Press Alcohol waste as Drying result with Various Temperature Variation Yusta, G. Maria; Meitiniarti, V. Irene; Kristiani, E.B.E.
Journal Of Natural Sciences And Mathematics Research Vol 2, No 2 (2016): Volume 2, Nomor 2, 2016
Publisher : Faculty of Science and Technology, State Islamic University Walisongo Central Java

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (952.257 KB) | DOI: 10.21580/jnsmr.2016.1.2.1664

Abstract

The content of yeast in industrial solid waste fermented beverages, has not been widely noticed and utilized, whereas many sources of nitrogen, vitamins and minerals are still present in this yeast. One of the utilization of waste yeast is processed into autolysis yeast extract. The drying process is often done using a spray dryer, but the process is costly so it is not efficient. In this study, we want to determine the temperature and drying time appropriate for maintain of yeast extract quality of yeast press. From this research, we concluded that a drying oven at 60 ° C for 6 hours is drying which does not damage the nutrient (protein, vitamine B2, carbohydrate, prolin, and lysin content) in yeast extract.©2016 JNSMR UIN Walisongo. All rights reserved.
Secondary Metabolites and Antioxidant Activity of Ethanolic Extract of Faloak (Sterculia quadrifida) Dillak, Hory Iramaya; Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20736

Abstract

Faloak (Sterculia quadrifida) is a medicinal plant used by the people of East Nusa Tenggara to treat lumbago, liver dysfunction and to restore the stamina. The research aims were to determine the qualitative and quantitative content of flavonoids, phenols, and tannins, as well as to examine the antioxidant activity of roots, stem barks, leaves, fruits and seeds extracts of faloak plant. Each organ was extracted with ethanol 70% using the maceration method. The qualitative content of bioactive compounds was determined using the phytochemical screening method. The determination of bioactive compounds concentration was using spectrophotometric methods and antioxidant activity was using the DPPH method. The result of phytochemical screening showed that all of the extracts were exhibit phenols compounds, but the flavonoids and tannins were only found in roots, barks, leaves, and fruits extracts. The quantitative content of total flavonoids of roots, barks, leaves, fruits, and seeds was 48.09; 62.76; 12.56; 11.91 and 1.55 mg/g, while the phenols total content were 82.90; 45.37; 3.43; 29.50 and 2.89 mg/g. Tannins total content were 71.26; 59.64; 10.52; 13.18 and 14.12 mg/g samples respectively. The stem barks and roots extracts showed a very strong antioxidant activity, while leaves, fruits, and seeds extracts belong to the strong category.  The potential of faloak as an antioxidant has been widely studied, especially in the stem bark. Studies on the antioxidant activity of roots, leaves, fruits, and seeds can provide new information about the benefits of phaloac plants as a source of natural antioxidants.
PERTUMBUHAN ARTEMISIA VULGARIS SECARA KULTUR PUCUK PADA MEDIUM DENGAN KANDUNGAN MIOINOSITOL DAN EKSTRAK KHAMIR Kasmiyati, Sri; Herawati, Maria M.; Kristiani, Elizabeth B.E.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.038 KB) | DOI: 10.24002/biota.v13i2.2672

Abstract

The effects of mioinositol and yeast extract were studied to assess their influence on growth of plantlets of Artemisia vulgaris by shoot culture. The plants regeneration of A. vulgaris were established by removing the nodes of stem and growing in MS multiplication medium with 1 ppm kinetin and 1 ppm NAA for 4 weeks. Shoots were induced for roots on MS treatment medium supplemented with mioinositol and yeast extract, added with 2 ppm IBA. Combination of four levels mioinositol concentration (mg/l): 100, 200, 300, and 400, and four levels of yeast extract concentration (mg/l): 0, 200, 300, and 400 were simultaneously added. Plantlets (2 weeks) were sub cultured on semi liquid MS medium. Plantlets were harvested on 6 weeks old. Measured parameters were fresh weight of plantlets. Data were analyzed using ANOVA followed by HSD test (p=95%). The results showed that the treatment of mioinositol and yeast extract were not significantly influenced on fresh weight of plantlets. Yeast extract was not influenced the growth of plantlets. The growth and morphogenesis of plantlets A. vulgaris were induced in treatment 100 ppm mioinositol, and addition mioinositol were higher than 100 ppm not significantly influenced the growth of plantlets.
EFFECT OF INDUCED POLYPLOIDY ON PLANT GROWTH, CHLOROPHYLL AND FLAVONOID CONTENT OF ARTEMISIA CINA Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok; Herawati, Maria Marina
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.361 KB) | DOI: 10.15294/biosaintifika.v12i1.22548

Abstract

Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.
The Study of Morphology, Phytochemical, and Distribution of Pittosporum moluccanum in Mount Merbabu National Park (TNGM)For the Development of Educationl Teaching Materials Setiyarini, Chatarina Titik; Kristiani, Elizabeth Betty Elok; Yulianto, Sri
International Journal of Active Learning Vol 5, No 1 (2020): April 2020
Publisher : International Journal of Active Learning

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.072 KB) | DOI: 10.15294/ijal.v5i1.22768

Abstract

This study aims to 1) observe the morphology of P. moluccanum, 2) analyze the content of chemical compounds in P. moluccanum, and 3) determine Normalized Difference Vegetation Index (NDVI) and Enhanced Vegetation Index (EVI) of P. moluccanum. The research was conducted in the form of morphology studies by conducting field studies, phytochemical compounds sample test by conducting sample preparation, chemical screening, and detection of aromatic compounds and quercetin, and remote sensing of Landsat 8.0 OLI satellite imagery. The results of morphological studies show that P. moluccanum have the same characteristics as plants in the literature, whereas the phytochemical test shows that P. moluccanum’s leaves and fruit contain flavonoids, tannins, saponins, steroids, and alkaloids. These compounds are known to have many functions for medicines. NIR analysis shows that leaf and fruit samples are detected to have aromatic compounds (essential oils) which are widely used as raw materials for fragrance oils or cosmetics. The results of remote sensing using NDVI in the Selo region, Boyolali produce a maximum vegetation index value of 0.464, meaning that the area has a high level of greenness, while the EVI used to see the level of health and fertility of plants in August 2018 shows the same figure, which is 0.464, meaning that the plant is in healthy condition. Remote sensing with satellite imagery helps analyze the distribution of sample plants in a large area. The results of this study can be used as a development of teaching materials both at junior and senior high school levels.
Korelasi Antara Aktivitas Antioksidan dengan Kandungan Senyawa Fenolik dan Lokasi Tumbuh Tanaman Celosia argentea Linn. Wardani, Yulia Kusma; Elok Kristiani, Elizabeth Betty; Sucahyo, S
Bioma : Berkala Ilmiah Biologi Vol. 22, No 2, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.22.2.136-142

Abstract

Celosia argentea Linn. is a herbaceous plant of Amaranthaceae family. The plant has antioxidant property. The antioxidant capacity is determined by the existence of secondary metabolite of which phenolics compound is one of them. This research aim was to determine the correlation between phenolics compound content and the growing environment with the antioxidant activity of Celosia argentea Linn. The plants were obtained from three different locations in Salatiga area included Ngronggo Village Landfill (TPA), Jalan Raya Lingkar Selatan (JLS), and in Blotongan Housing Complex areas (PB). The fresh leaves of the plant were extracted by maceration method using ethanol 96% in the room temperature for 48 hours. The determination the phenolics content was using Folin-Ciocalteu method, while antioxidant activity using DPPH assay. The phenolis content of C. argentea Linn. leaves were 10,22; 6,99; 4,97 ppm from TPA, JLS, and PB respectively, while the IC50 values of antioxidant activity were 2,98; 3,18; and 3,35 ppm respectively. There were a correlation between antioxidant activity and phenolics compound with the growing environment of C. argentea Linn. The higher of phenolics compound content, the lower the IC50 value (higher an antioxidant activity). The phenolics content was also has a strong correlation with environment.  
Pengaruh Lokasi Tumbuh Terhadap Kadar Flavonoid, Fenolik, Klorofil, Karotenoid Dan Aktivitas Antioksidan Pada Tumbuhan Pecut Kuda (Stachytarpheta Jamaicensis) Utomo, Daniel Setyo; Elok Kristiani, Elizabeth Betty; Mahardika, Anggara
Bioma : Berkala Ilmiah Biologi Vol. 22, No 2, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.22.2.143-149

Abstract

Indonesia is a tropical country with high biodiversity of flora and fauna. Jamaica vervain (Stachytarpheta Jamaicensis) is abundance in Indonesia. The purpose of this study is to compare the effect of the habitats to the product of secondary metabolit. Samples were collected from different locations in Central Java: Kopeng (K) (1350 mdpl) and Plamongan Indah, Semarang City (S) (4 mdpl). Fresh leaves are macerated at the room temperature for 48 hours in ethanol solvent. The level of compound were determined by spectrophotometry, while antioxidant activity were analyzed with DPPH method. Data were analyzed using SPSS with the T-test. Levels of flavonoids, phenolic, chlorophyll, and carotenoids (respectively) in the S sample are: 37.11; 8.74; 122.49 and 9.76 mg/L, while the K sample: 20.44; 3.81; 103.74 and 10.23 mg/L. The IC50 value of S sample is 1.17 mg/ml and 2.17 mg/ml in K sample. The results of measurements of environmental conditions in S and soil pH values are 7.0 and 6.8; light intensity of 6300 and 6150 lx, temperatures in the range of 27-38oC and 13-24oC. The growing location affect the levels of flavonoids, phenolics, and antioxidant activity in S. jamaicensis but do not affect levels of chlorophyll and carotenoids.
The Antioxidant Content and Activity of Various Plant Organs of Kitolod (Isotoma longiflora) Egarani, Grahita Rezhi; Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23888

Abstract

Antioxidants play a crucial role in human health owing to their ability to neutralize oxidative reactions. Kitolod (Isotomalongiflora) is one of the medicinal plants that can be used as a source of antioxidant. The aim of study wasto compare the content of antioxidant compounds (flavonoids, phenolics, chlorophylls, carotenoids, and ascorbic acid) in various organs of the kitolod plant. The plant organs studied includingroots, stems, leaves, flowers, and fruits. The extraction of each plant organ wasconducted by maceration technique using ethanol as a solvent at the room temperature for 48 hours. The analysis of total flavonoids, phenolics, and ascorbic acid was performed by colorimetry method using AlCl3, Folin, and sulfosalicyclic acid reagent respectively, while chlorophylls and carotenoids was determined by direct colorimetry method with methanol as a solvent. The antioxidant activity of various plant organs of kitolod was analyzed using the DPPH method. The highest total flavonoid, phenolic, chlorophyll, and carotenoid compounds werefound in leaves, respectively by 10.48, 1.46, 7.25, and 56.98 ppm. The highest ascorbic acid content and antioxidant activity were obtained fromfruits.The research findings provide new and important information aboutthe contents and antioxidant activity of the secondary metabolites (flavonoid, phenolic, chlorophyll, carotenoid, and ascorbic acid) in each organ of kitolod plant. The information from the results of this study can be used to increase the medicinal value ofkitolod plants.
Antibacterial Activity and Flavonoids Content of Artemisia cina Berg. ex Poljakov Ethyl Acetate Extracts Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok; Herawati, Maria Marina; Sukmana, Andreas Binar Aji
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27665

Abstract

The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial  potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent. 
The Combination of Phyllanthus niruri, Euphorbia hirta, and Loranthus sp. as a Source of Antioxidant Agents Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.30718

Abstract

Meniran (Phyllanthus niruri), patikan kebo (Euphorbia hirta), and benalu (Loranthus sp.) have often been used by people as medicinal plants. This research aimed to measure the levels of flavonoids, phenolics, and ascorbic acid compounds, as well as the Free Radical Scavenging (FRS) activity of ethyl acetate extract from the mixture of P. niruri, E. hirta, and Loranthus sp. The FRS activity was measured with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The level of compounds was measured by using the spectrophotometry method with specific reagents. The result of the FRS activity in ethyl acetate extract from the mixture of P. niruri, E. hirta, and Loranthus sp. varied depending on its dose. The measure of FRS in the P. niruri: E. hirta: Loranthus sp 0:0:1 (K-OOL) composition showed a strong result with a value of IC50 97.2 ± 2.1 ppm, while in the 0:0.5:0.5 (K-OEL) composition it was moderate with a value of IC50 147.6 ± 6.5 ppm. The other compositions showed weak and inactive results. The K-OOL composition had the highest flavonoid and phenolic content that were 298.8±0.00 mg QE/g extract and 141.5±2.85 mg GAE/g extract respectively. The composition with the highest ascorbic acid content (298.8±0.00 mg/g extract) was K-OEO composition. So far, research on medicinal plants is still limited to one type of plant. The combination of several types of plants in several formulations allows obtaining a composition that can produce maximum antioxidant capacity. Therefore, this research is expected to produce a combination formulation of various types of medicinal plants that have the K-OOL composition very strong antioxidant activity and can be used as herbal medicines.
Co-Authors Agung Rimayanto Gintu Agung Rimayanto Gintu Agung Rimayanto Gintu Andreas Binar Aji Sukmana Anggara Mahardika Ari Hasna Widyapuspa Egarani, Grahita Rezhi Hartati Sulastri Jeanelsin A.P Langgori Lilik Linawati Maria Marina Herawati Setiyarini, Chatarina Titik Sri Kasmiyati Sri Yulianto Sucahyo, S Utomo, Daniel Setyo VINCENTIA IRENE MEITINIARTI Wardani, Yulia Kusma Yayi Suryo Prabandari Yohanes Martono Yusta, G. Maria