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All Journal HAYATI Journal of Biosciences Jurnal Kimia (Journal of Chemistry) Jurnal Promosi Pendidikan Ekonomi Indonesian Journal Of Environmental Science and Technology Chimica et Natura Acta JURNAL PHOTON Jurnal Inovasi Pendidikan dan Sains Jurnal Teknik Kimia USU
Saryono
Universitas Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Nursing Physics

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Phytoremediation with Acasia (Acasia crassicarpa) on peat soil using fly ash and dreg as ameliorants Rejeki, Yayuk Sri; ', Nelvia; ', Saryono
Indonesian Journal of Environmental Science and Technology Vol 1, No 1 (2014)
Publisher : Universitas Riau

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Abstract

Utilization of fly ash and dreg on peat soil to improve soil fertility is potential because there are largeamount of fly ash and dreg as well as the existence of critical. This study was aimed to determine the effect of fly ash and dreg on the growth and the accumulation  of metals Ba, Cd, Cr, Ni, Pb, and Se of Acacia crassicarpa and its absorption  efficiency in the peat soil. The results showed that the addition of fly ash and dreg with dif­ ferent doses and different  harvesting  time influenced  on the growth and the accumulation  of heavy metals in Acacia crassicarpa. The results of the study also indicated that there were differences in the absorption efficien­ cy of heavy metals by Acacia crassicarpa.Keywords: fly ash, dreg, metal accumulation, absorption efficiency, Acacia crassicarpa.
Pollutant dispersion modelling (Dust, CO, NOx, and SOx) from palm oil mill stack (Case Study of PT. Mustika Agung Sawit Sejahtera) Hermantika Sari, Nadya Hermantika x; Tarumun, Suardi; ', Saryono
Indonesian Journal of Environmental Science and Technology Vol 1, No 1 (2014)
Publisher : Universitas Riau

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Abstract

Pollutant dispersion  modeling study of a stack is one of the efforts in addressing  the problems  thatemissions of the palm oil industry can match the capacity of the environment, so it will not impact on the envi­ ronment  and society.  Screeen  view SCREEN3  software,  based of Gaussian  dispersion  method  for dust, CO, NOx, and SOx pollutant,  was used for pollutant  dispersiion modeling.  The input parameters  in the simulation were the rate of emission of pollutants  emitted from the stack, the air velocity around the source of emissions, atmospheric stability factor, and the topography of the site. While the expected output was a visualization of dis­ tribution of pollutants concentration  in the form of 2-dimensional  plane-shaped  contour that came with concen­ tration values of the distance from the source  of emission. Modeling  in this study was conducted  with several scenarios that the average meteorological  conditions at the time of measurement and the worst conditions. The results showed that the dominant wind direction was to the south with an average speed of 5.87 m/s. The result­ ing emission rate was 36.4 g/s. As for the pollutants, CO was 13.84 g/ s, SOx was 0.213 g/s and NOx 24.49 g/ s. Emission maximum distance was 1340 m from the stack with dust concentration  at 133.9 ug/m3   The concentra­ tions of CO, NOx, and SOx were 50.90 ug/m3   90.06 ug/m3;  0.7833 ug/m3  respectively.Keywords: environment,  pollutant  dispersion  modeling,  stack,  palm oil mill,  Gaussian  dispersion  method,  Screen  viewScreen3
BEBERAPA MIKROORGANISME YANG MENGHASILKAN ENZIM INULINASE, ISOLASI DAN KARAKTERISASI ENZIM DARI Aspergillus flavus Gmn11.2 GALUR LOKAL Saryono Saryono; Fitriani Fitriani; Ukun M.S. Soedjanaatmadja
Chimica et Natura Acta Vol 4, No 3 (2016)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.219 KB) | DOI: 10.24198/cna.v4.n3.11030

Abstract

Indonesia merupakan negara tropis yang  kaya akan berbagai tumbuhan dan mikroorganisme. Beberapa mikroorganisme memiliki kemampuan untuk menghasilkan enzim inulinase yang sangat bermanfaat untuk industri pangan dan farmasi. Inulinase adalah enzim yang menghidrolisis inulin menjadi fruktosa atau frukto-oligosakarida. Untuk mengisolasi inulinase dalam jumlah yang cukup banyak dari tumbuhan cukup sulit, oleh sebab itu inulinase mikrobial merupakan obyek penelitian yang sangat menarik bagi para peneliti. Tujuan penelitian ini adalah isolasi dan indentifikasi berbagai jenis jamur lokal yang potensial menghasilkan inulinase, serta isolasi dan pemurnian inulinase dari A. flavus Gmn11.2 galur lokal. Mikroorganisme A. flavus Gmn11.2 galur lokal dikembangbiakan pada media fermentasi  skala labu 250 mL, yang mengandung induser inulin selama 84 jam pada suhu 37oC. Enzim inulin (ekstraselular) yang diproduksi selanjutnya diisolasi dan dimurnikan melalui tahapan fraksionasi amonium sulfat pada rentang kejenuhan 40-80%, kromatografi kolom filtrasi Gel Sephadex G-25 (100 × 2 cm), kromatografi kolom penukar ion DEAE selulosa (20 × 1,6 cm), dengan elusi gradien 0,01-0,1 M buffer natrium fosfat pada laju alir 3 mL/menit. Enzim murni hasil isolasi kemudian ditentukan kondisi optimum aktivitas serta parameter kinetikanya. Hasil Penelitian menunjukkan bahwa dari berbagai mikroorganisme yang memiliki potensi untuk menghasilkan enzim inulinase dengan aktivitas yang tinggi adalah Aspergillus clavatus (BG5), Fusarium solani (PB3), Fusarium sp2 (LB2), dan A. flavus (ML2). Setelah melalui tahapan kromatografi penukar ion, diperoleh enzim inulinase yang cukup murni yang ditandai dengan intensitas pita hasil SDS elektroforesis, dengan peningkatan faktor kemurnian 15,66 kali dibandingkan dengan ekstrak enzim kasar. Adapun aktivitas  enzim inulinase hasil isolasi dari A. flavus Gmn11.2 galur lokal, bekerja pada kondisi optimum: pH 5,0, suhu 50°C dan waktu inkubasi 4 jam. Parameter kinetika enzimatik dari inulinase diperoleh harga KM33 mg/L dan Vmaks 1,8 x 10-3 µmol/menit. Dari hasil elektroforesis SDS poliakrilamida menunjukkan enzim inulinase dari A. flavus Gmn11.2 galur lokal, memiliki berat molekul (Mr) 138 kDa, terdiri dari dua sub-unit polipeptida (dimer) dengan Mr masing-masing 72 dan 66 kDa.
PENGEMBANGAN TECHNOPRENEURSHIP DI UNIVERSITAS RIAU Sumarno Sumarno; Saryono Saryono; Gimin Gimin
PROMOSI: Jurnal Program Studi Pendidikan Ekonomi Vol 5, No 2 (2017): PROMOSI
Publisher : UNIVERSITAS MUHAMMADIYAH METRO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/ja.v5i2.1217

Abstract

Nation or state will prosper if it have at least 2% of entrepreneurs.According to the Minister of Cooperatives and SMEs, entrepreneurs in Indonesia are still around 1.6%.For that, we need to developtechnopreneurship in order to produce highly competitive entrepreneurs.The purpose of this research is to get information about technopreneurship development in University of Riau.The research was conducted on the application of entrepreneurship at University of Riau, Pekanbaru.Data were collected through interviews, observation, and documentation, analyzed descriptively.The results showed that the number of students who have a business plan in accordance with their academic disciplines on average only 5%.The factor is the implementation of technopreneurship development has not been optimal.Lecturers have not emphasized the creative ability of students in accordance with academic science, while entrepreneurship lecturers only instill knowledge, insight, and entrepreneurial interest.Units and support student entrepreneurship programs have not direct students' creative business ideas to the field that were appropriate to their academic sciences.
POTENSI TIGA ISOLAT BAKTERI INDIGEN DARI KABUPATEN SIAK PROVINSI RIAU DALAM MENDEGRADASI NAFTALENA R. Novianty; B. Antika; . Saryono; A. Awaluddin; N. W. Pratiwi
Jurnal Kimia (Journal of Chemistry) Vol.14 No.1 Januari 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.61 KB) | DOI: 10.24843/JCHEM.2020.v14.i01.p16

Abstract

Naphthalene is a group of Polycyclic Aromatic Hydrocarbons (PAHs) which is carcinogenic when presents in the environment. The purpose of this study was to determine the growth response and test the effectiveness of three isolates of indigen bacteria from the Joint Operation Agency area of ??PT. Siak Bumi Pusako-Pertamina Hulu (BOB BSP-PHE) in degrading naphthalene. The three isolates were tested in a Minimum Media (MM) liquid containing naphthalene with a final concentration of 0.2 mM and was incubated for 7 days. Optical Density (OD) and the degradation ability were analyzed using a UV-Vis spectrophotometer. The results showed that Pseudomonas sp. LBKURCC149 was able to grow in media containing naphthalene. Naphthalene is used as the only source of carbon and is able to degrade naphthalene by 21.98%. Keywords: biodegradation, indigenous, naphthalene, Pseudomonas sp.
Molecular Identification, GC-MS Analysis of Bioactive Compounds and Antimicrobial Activity of Thermophilic Bacteria Derived from West Sumatra Hot-Spring Indonesia Zona Octarya; Titania Tjandrawati Nugroho; Yuana Nurulita; Saryono
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.549-561

Abstract

Thermophilic bacteria are a source of bioactive compounds that have many benefits for human life. One of them is as a source of antimicrobials. This research aimed to identify and characterize the promising thermophilic bacterial isolates by analyzing bioactive compounds and their potential as antimicrobial agents. Thermophilic bacteria with the code LBKURCC were taken from the collection of the Biochemistry Laboratory of the University of Riau. Forty-four purified strains of thermophilic bacteria were tested for antimicrobial ability. These thermophilic bacteria were taken from hot springs located in the Sumatra provinces of West Sumatra and Riau. Strain LBKURCC218 isolated from Rimbo Panti hot springs in West Sumatra was chosen to further investigate antimicrobials production. Isolates of hot spring bacteria that produced the highest antimicrobial were identified by comparing the similarity of the 16S rRNA gene sequences. BLAST result and phylogenetic tree showed that the selected thermophilic bacterial strain was similar to Bacillus paramicoides with the similarity index of 99.93%. Analysis of bioactive compounds of the ethyl acetate extract of liquid cultures of B. paramycoides LBKURCC218 showed the best producer of antimicrobial compounds compared to other isolates. The most identified compounds from the ethyl acetate extract were Dodecanoic acid, representing 23.62% of the total compounds, followed by 11-Dodecanoic acid at 17.84%. Ethyl acetate extract of B. paramycoides LBKURCC218 has a high inhibition zone against Escherichia coli, Staphylococcus aureus, and Candida albicans.
OPTIMASI PERTUMBUHAN ISOLAT FUNGI INDIGEN Penicillium sp. LBKURCC153 PENDEGRADASI NAFTALENA MENGGUNAKAN GLUKOSA SEBAGAI KOSUBSTRAT PADA MINIMAL MEDIUM Aisyah Fitrida; Riryn Novianty; Saryono Saryono; Amir Awaluddin; Nova Wahyu Pratiwi
JURNAL INOVASI PENDIDIKAN DAN SAINS Vol 1 No 1 (2020): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Nahdlatul Wathan Mataram

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Abstract

Naftalena merupakan salah satu polutan yang sulit untuk didegradasi karena termasuk kedalam kelompok PAH dan bersifat karsinogenik. Tujuan dari penelitian ini adalah untuk menguji efektifitas isolat fungi indigen dari Provinsi Riau untuk mendegradasi senyawa toksik naftalena dengan penambahan glukosa sebagai kosubstrat pertumbuhan. Degradasi naftalena oleh isolat fungi dipengaruhi oleh beberapa parameter, diantaranya biomassa, pH dan persentase degradasi yang diukur pada 0, 4, 8, 12 dan 16 hari inkubasi. Berdasarkan dari parameter biomassa isolat fungi Penicillium sp. LBKURCC153 mengalami fase eksponensial sampai hari ke-16. Pada fase eksponensial terjadi pertambahan jumlah sel secara maksimum yang menunjukkan respon positif terhadap penggunaan glukosa dan naftalena sebagai sumber karbon dan energi. Isolat mengalami penurunan pH dari 7 sampai 3,8 yang artinya ada aktivitas pertumbuhan yang menghasilkan asam-asam organik dari proses degradasi naftalena. Isolat juga mampu mendegradasi naftalena sebesar 27,5% oleh Penicillium sp. LBKURCC153 pada media dengan penambahan glukosa pada waktu optimum selama 4 hari inkubasi. Kesimpulannya, penambahan kosubstrat glukosa dapat meningkatkan persentase degradasi naftalena oleh Penicillium sp. LBKURCC153
PENGARUH SUHU PADA PROSES PENGOMPOSAN PELEPAH SAWIT MENGUNAKAN ISOLAT LOKAL Pseudomonas stutzeri (LBKURCC 54 DAN LBKURCC 59) Suci Apriani; Amir Awaluddin; - Saryono
Sistem Informasi Vol 6 No 01 (2015): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v6i01.474

Abstract

Saat ini, pelepah kelapa sawit merupakan limbah pertanian yang belum dimanfaatkan secara optimal. Pada penelitian ini, limbah pelepah kelapa sawit dikonversi menjadi kompos dengan cara fermentasi. Proses pengomposan dilakukan menggunakan kombinasi bioaktivator lokal ditambah kotoran ayam yang berfungsi menyediakan nutrisi bagi bioaktivator. Bioaktivator sebagai starter yang digunakan merupakan kombinasi dari dua Isolat yaitu Pseudomonas stutzeri (LBKURCC 54 dan 59) yang disubkultur pada Nutrient Broth dan difermentasi selama 7 hari menggunakan media bibit. Substrat diinokulasi 10 % starter dari total bahan. Untuk mempelajari kemampuan bioaktivator lokal dalam mendegradasi pelepah kelapa sawit dilakukan variasi sebagai berikut: (1) sampel dengan dan tanpa starter (2) sampel dengan dan tanpa pembalikan. Kualitas kompos terbaik yang dihasilkan dari sampel yang menggunakan starter dengan pembalikan tiga hari sekali. Nilai rasio C/N yang merupakan indikator penentu kematangan kompos, menurun hingga akhir pengomposan dan telah sesuai dengan standar SNI yaitu 13,46.
OPTIMASI PRODUKSI DAN IDENTIFIKASI SENYAWA ANTIMIKROB DARI JAMUR ENDOFIT Sporothrix sp LBKURCC43 TANAMAN DAHLIA (Dahlia variabilis) - Paslun; - Saryono; Chiristine Jose
Sistem Informasi Vol 7 No 01 (2016): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v7i01.557

Abstract

Dahlia tubers contain fungus Sporothrix sp which have antimicrobial activity.The objective of this research was to determine the agitation that effect the activity of antimicrobial in malt extract broth (MEB). The result showed that agitation of 150 rpm have the antimicrobial activity against Staphylococcus aureus with 21.0 mm and Escherichia colii with 12.8 mm. The identification of function group showed the extract contained O-H, C=O and Amide with UV and IR spectroscopy.
PENGARUH SUHU TERHADAP PRODUKSI ASAM LEVULINAT DARI INULIN UMBI DAHLIA (Dahlia Sp.) Rosa Murwindra; Saryono Sikumbang; Amir Awaliddin; Amilia Linggawati
Sistem Informasi Vol 7 No 01 (2016): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v7i01.573

Abstract

Dahlia is a floriculture plant with tuber that rich in inulin. Inulin is soluble in hot water and it will settle on cool temperature in alcohol. The inulin extraction to use hot water from dahlia tuber take from Bukittinggi of West Sumatra. Extraction result from 100 g dahlia tuber is 4% inulin (w/w dahlia tuber), such as inulin flour is white gray. As qualitative test with Seliwanoff and Nelson-Somogyi methods, that inulin positive contain of fructose and reducing sugar. This inulin can be converted into levulinic acid using acid catalyst at high temperature. Production of levulinic acid with variate reaction temperature from 130 – 170 oC. The reaction of inulin convertion is done in ampul that burned in oven. In this experiment, conversion of inulin to be levulinic acid is high 26,25% (w/w inulin) using acid catalyst of hydrochloride acid 1 M and heating 170 oC about 15 minutes.
Co-Authors - Paslun A. Awaluddin Aisyah Fitrida Amilia Linggawati Amir Awaliddin Amir Awaluddin B. Antika Chiristine Jose Fitriani Fitriani Gimin Gimin N. W. Pratiwi Nadya Hermantika x Hermantika Sari, Nadya Hermantika x Nelvia Nelvia Nova Wahyu Pratiwi Nova Wahyu Pratiwi Novianty, Riryn R. Novianty R. Ukun M.S. Soedjanaatmadja Riryn Novianty Rosa Murwindra, Rosa Sefni Hendris Suardi Tarumun Suci Apriani Sumarno Sumarno Titania Tjandrawati Nugroho Yayuk Sri Rejeki, Yayuk Sri Yuana Nurulita Zona Octarya