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. Warsinah
Jurusan Farmasi, Fakultas Kedokteran dan ilmu kesehatan Unsoed Purwokerto
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology

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Antiproliferative Effect and Apoptosis Induced in Human Cell Lines by Bruguiera gymnorhiza Barks Methanol Extract Warsinah, .; Sismindari, .; Susidarti, Ratna Asmah
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

The Antiproliferative effects of methanol extract from Bruguiera gymnorhiza (B. gymnorhiza) barks were tested in vitro against three human cell lines: Hela, Raji and Myeloma cells. The extract was found to have antiproliferative effects against Hela, Raji and Myeloma cells with an IC50 value of 133, 504 and 384 µg/mL, respectively. The antiproliferative test was then performed on Hela, Raji,  and  Myeloma  cells.  Cytotoxicity  assay  of  the  extract  was  then  determined  using  MTT method.  There  were  some  indications  of  apoptosis,  such  DNA  fragmentation,  as  assessed  by acrydine orange- ethidium bromide staining. These results indicate that extract from B. gymnorhiza barks can induce apoptosis in human cell lines.Key words: Antiproliferative, Apoptosis, B. gymnorhiza
Identifikasi Senyawa Antikanker dari Fraksi Kloroform Kulit Batang Bruguiera gymnorhiza dan Aktivitas Sitotoksiknya Warsinah, .; Sismindari, .; Susidarti, Ratna Asmah
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 2 (2012)
Publisher : Indonesian Research Gateway

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Bruguiera gmnorhiza is one of mangrove plant that has not been widely studied as potential anticancer compounds. Previous research noted that the methanol extract of the bark of B.gymnorhiza are cytotoxic towards HeLa cells. The purpose of this study was to determine the compounds in the chloroform fraction and cytotoxic activity in HeLa cells. Stem bark of B. gumnorhiza was extracted with methanol, further fractionation by partitioning method, using of the solvent h-hexane and chloroform. Fraction of chloroform was evaporated, the tested cytotoxic activity against HeLa cells by MTT method and apoptotic induction by double staining method. Faction levels are used for 500, 250, 125, 62.5 and 31.25 mg/mL with 24-hour incubation time. Subsequent fractions were identified by GC-MS method. The result showed that the chloroform fraction contains 21 compounds, 6 in the form of fatty acids and terpenoids that have anticancer cytotoxic activity (IC50) of 134 ug/mL with apoptotic mekanism.Keywords: Apoptosis, Bruguiera gymnorhiza, cytotoxic, Fractionation, HeLa cell
Identifikasi Senyawa Antikanker dari Fraksi Kloroform Kulit Batang Bruguiera gymnorhiza dan Aktivitas Sitotoksiknya Warsinah, .; Sismindari, .; Susidarti, Ratna Asmah
Jurnal Farmasi Indonesia Vol 6, No 2 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.176 KB) | DOI: 10.35617/jfi.v6i2.129

Abstract

Bruguiera gmnorhiza is one of mangrove plant that has not been widely studied as potential anticancer compounds. Previous research noted that the methanol extract of the bark of B.gymnorhiza are cytotoxic towards HeLa cells. The purpose of this study was to determine the compounds in the chloroform fraction and cytotoxic activity in HeLa cells. Stem bark of B. gumnorhiza was extracted with methanol, further fractionation by partitioning method, using of the solvent h-hexane and chloroform. Fraction of chloroform was evaporated, the tested cytotoxic activity against HeLa cells by MTT method and apoptotic induction by double staining method. Faction levels are used for 500, 250, 125, 62.5 and 31.25 mg/mL with 24-hour incubation time. Subsequent fractions were identified by GC-MS method. The result showed that the chloroform fraction contains 21 compounds, 6 in the form of fatty acids and terpenoids that have anticancer cytotoxic activity (IC50) of 134 ug/mL with apoptotic mekanism.Keywords: Apoptosis, Bruguiera gymnorhiza, cytotoxic, Fractionation, HeLa cell
Co-Authors . Sismindari Ratna Asmah Susidarti