Article Per Year (5 Year)
p-Index From 2019 - 2024
2.155
P-Index
This Author published in this journals
All Journal Indonesian Journal of Cancer Chemoprevention Jurnal Ilmu Kesehatan Jurnal Kesehatan Tambusai Journal of Fundamental and Applied Pharmaceutical Science BAKTIMU : Jurnal Pengabdian Kepada Masyarakat Medimuh : Jurnal Kesehatan Muhammadiyah Jurnal Ilmiah Bakti Farmasi Medical Sains : Jurnal Ilmiah Kefarmasian CERATA Dedikasi : Jurnal Pengabdian Lentera
Ismanurrahman Hadi
Prodi Sarjana Farmasi, STIKES Muhammadiyah Cirebon
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

Published : 14 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Indonesian Journal of Cancer Chemoprevention

 1 
Pentagamavunon-0 (PGV-0) Enhance Cytotoxic Effect of Doxorubicin through Increasing of Apoptosis, Senescence and ROS Level on Triple Negative Breast Cancer 4T1 Ismanurrahman Hadi; Riris Istighfari Jenie; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 11, No 1 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss1pp7-15

Abstract

TNBC, one of the sub type of breast cancers was widely known with high tumorigenic and poor prognosis than others. The development of combination agent (co-chemotherapy) with doxorubicin for chemotherapy of TNBC were carried out to decrease doxorubicin side effect and resistance in cancer. This present study aims to explore the co-chemotherapeutic properties of PGV-0 and investigate induction of doxorubicin on apoptosis, senescence and ROS against TNBC. 4T1 Cell line were used as a TNBC in vitro model. Cytotoxic measurement was performed using MTT assay resulting in IC50 values of 52 μM. Meanwhile, the combination of doxorubicin and PGV-0 showed synergistic effect which decreased cell viability of 4T1 better than single treatment of doxorubicin. Apoptosis analysis was performed using annexin V/PI assay indicated that the combination treatment of PGV-0 and doxorubicin increased apoptosis evidence. Senescence detection was carried out using senescence-associated-β galactosidase (SA-β-gal) assay. The results showed that a single treatment of PGV-0 induced cellular senescence and increased senescence cells in combination treatment. Moreover, DCFDA staining showed that PGV-0 increased ROS level at single treatment, whereas combination treatment increased ROS intracellular compared to the positive control of doxorubicin. Based on these results, PGV-0 has potential as a co-chemotherapeutic candidate on TNBC.Keyword: 4T1, PGV-0, Co-chemotherapy, Cytotoxic, Senescence, Apoptosis, ROS
Co-Authors Ade Irawan Alvia Nuraeni Amelia Zannah Antika Mardhotillah Meilian Arief Kusuma Wardani Asya Nurfazera Ayu Zakkiyah Dadan Hidayattullah Deby Nurlutfiana Edy Meiyanto Erawati Erawati Flori Sindi Agwiana Hilda Nurul Fauzia Like Efriani Mahfud Anwar Mailatul Ilal Haq MARIAM ULFAH MARIAM ULFAH ULFAH Muhamad Rifki Purnama Nirma Rohmatika Perdana Priya Haresmita Putri Wulan Dari Riris Istighfari Jenie Samsudin Azhari Shalma Risqi Amanda Teguh Adiyas Putra Tonasih Tonasih