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All Journal Jurnal Pembelajaran dan Biologi Nukleus
Reinny Silvana Tuegeh
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

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Antagonistic and Antibacterial Activity of Stapylocossus aureus and Isolates of Oral Bacteria from the Endogenous Fungus Apis dorsata Binghami Nest Mokosuli Yermia Semuel; Masje Wurarah; Reinny Silvana Tuegeh
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 2 (2022): Jurnal Pembelajaran Dan Biologi Nukleus Juli 2022
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i2.2739

Abstract

Apis Dorsata Binghami honey bee is a honey bee endemic to Indonesia, living naturally in the forests of Sulawesi. This study aimed to obtain isolates and characteristics of endogenous fungi antibiotic activity from Apis dorsata Binghami nest. The research consisted of isolation of fungi from beehives using potato dextro agar, pure culture of fungi, antagonist test and antibiotic test using disc diffusion method. Antibiotic test was performed on oral bacteria and Staphylococcus aureus. The results obtained six isolates of fungi from the nest of Apis dorsata Binghami, namely isolates FAB1, FAB2, FAB3, FAB4, FAB5 and FAB6. The results of the antagonist test showed that the isolates FAB2 and FAB3 had the best antagonist properties, while the FAB6 isolates had the weakest antagonist properties. The FAB2 isolate showed the best bacterial growth inhibition zone average for the isolates of oral bacteria and Staphylococcus aureus. The inhibition zone lasts up to 3 x 24 hours so that the activity of the bacteria is bactericidal. From the results of this study, it can be concluded that the endogenous fungus Apis dorsata Binghami is a potential source of antibacterial bioactives.
Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts Reinny Silvana Tuegeh; Herry Maurits Sumampouw; Meity Neltje Tanor; Yermia Semuel Mokosuli
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4514

Abstract

Beenest is rich in secondary metabolites because honeycombs, among others, are formed from plant resins (propolis). This study aimed to analyze differences in flavonoid content and in vitro antioxidant activity of Apis dorsata Binghami and Apis mellifera nest extracts. The samples used were A. dorsata from North Sulawesi and A. mellifera from South Sulawesi. The honeycomb was extracted using 95% ethanol solvent. Honeycomb extract was analyzed for its flavonoid content by the HPLC method, toxicity was tested by the BSLT method, and in vitro antioxidant activity was tested by the DPPH method. The results of the analysis of flavonoids showed that A. dorsata nest extract produced 21 types of compounds while A. mellifera produced 26 types of compounds. The toxicity test results showed that the A. dorsata nest extract had a better LC50 of 245,691 mg/l than the A. mellifera nest extract with an LC50 of 443,701 mg/l. The in vitro antioxidant test results of A. dorsata nest extract were more robust, namely IC 50 1.161 mg/l, compared to A. mellifera nest extract IC 50 2.404 mg/l. However, both were included in the category of powerful antioxidants. In vitro, anticancer test results on MCF-7 cells, A. mellifera nest extract was active with IC 50 100.02mg/l. Compared to A. dorsata extract, it was active with IC 50 102.217mg/l, but the two were not significantly different. Based on the analysis of flavonoid content, toxicity test, and antioxidant test, A.dorsata and A.mellifera honeycomb extracts have potential as in vitro antioxidants
Co-Authors Herry Maurits Sumampouw Masje Wurarah Meity Neltje Tanor Yermia Semuel Mokosuli