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All Journal Jurnal Fitopatologi Indonesia El-Hayah : Jurnal Biologi Jurnal Hortikultura Research Journal of Life Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Sri Widyaningsih
Balai Penelitian Tanaman Jeruk dan Buah Subtropika
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology

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Journal : Jurnal Fitopatologi Indonesia

 1 
Antifungal and Antibacterial Activity of Essential Oils of Kaffir Citrus Fruit Peel Extract Against Important Pathogens of Citrus Plants Triasih, Unun; Ikarini, Imro’ah; Widyaningsih, Sri; Wicaksono, Rudi Cahyo
Jurnal Fitopatologi Indonesia Vol 19 No 6 (2023): November 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.6.246-252

Abstract

Penyakit utama tanaman jeruk yang disebabkan oleh cendawan dan bakteri dapat menghambat pertumbuhan tanaman sehingga berdampak terhadap penurunan produksi tanaman. Pengendalian yang diterapkan selama ini kebanyakan menggunakan pestisida kimia sintetis yang berpotensi memberi dampak negatif pada lingkungan dan kesehatan. Minyak atsiri dari kulit buah jeruk purut (Citrus hystric) mempunyai kandungan senyawa yang berpotensi dalam menghambat pertumbuhan patogen pada tanaman jeruk. Tujuan dari penelitian ini adalah mengetahui aktivitas antifungal dan antibakteri minyak atsiri kulit buah jeruk purut pada lima konsentrasi 0.5%, 1%, 2%, 3%, dan 5% terhadap patogen tanaman jeruk, yaitu Colletotrichum gloeosporioides, Fusarium oxysporum, dan Xanthomonas axonopodis. Hasil penelitian menunjukkan bahwa semua konsentrasi minyak atsiri yang diujikan mempunyai daya hambat kategori rendah terhadap F. oxysporum, dan kategori sedang terhadap C. gloeosporioides. Pengujian terhadap X. axonopodis menunjukkan daya hambat kategori kuat untuk konsentrasi 3% dan 5%. Minyak atsiri kulit buah jeruk purut berpotensi sebagai antifungal dan antibakteri pada tanaman jeruk dan dapat digunakan sebagai teknologi alternatif pengendalian yang ramah lingkungan dan aman.
Comparison of Two Huanglongbing Detection Methods in Samples with Different Symptom Severity: Perbandingan Dua Metode Deteksi Huanglongbing pada Sampel dengan Tingkat Keparahan Gejala yang Berbeda Sariasih, Yenny; Subandiyah, Siti; Widyaningsih, Sri; Khurshid, Tahir; Mo, Jianhua; Donovan, Nerida
Jurnal Fitopatologi Indonesia Vol 20 No 4 (2024): Juli 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.4.174-186

Abstract

Huanglongbing or citrus greening in Asia caused by the pathogen Candidatus Liberibacter asiaticus (CLas) is one of the most devastating citrus diseases worldwide. This disease is one of the causes of decreased citrus production in Indonesia. Symptoms of huanglongbing in citrus plants in the field and greenhouses have different levels of severity. This study was conducted to detect CLas in several types of samples based on leaf symptoms using conventional and real-time PCR (qPCR). Three pairs of primers were used in this study, a pair of Las606/LSS for conventional PCR and two pairs for qPCR, namely Las931/LSS and Lj900F/Lj900R. The results showed that blotchy mottle is the most easily detected symptom of huanglongbing and found in fields and greenhouses. The Lj900F/Lj900R primer pair is more suitable for detecting CLas pathogens using qPCR than Las606/LSS based on the melting curve and Ct value that appear. qPCR detection is more accurate and sensitive even with lower DNA concentrations. The lower limit of Ct value of healthy leaf samples is 34.08. Citrus leaves are considered positive if the Ct value is less than 34.08. Ct value based on severity or scoring between HLB symptomatic leaves from the field and greenhouse showed a significant difference, i.e. the Ct value of symptomatic samples from the field was lower than that of greenhouse samples.
Co-Authors Abdul Latief Abadi Anton Muhibbudin Dewi, Ovilya Kusuma Minarma Dina Agustina, Dina Donovan, Nerida Farida Yulianti Ikarini, Imro’ah Khurshid, Tahir Mo, Jianhua Mutia Erti DWIASTUTI Nirmala Friyanti Devy Siti Subandiyah Triasih, Unun Unun Triasih wicaksono, rudi cahyo Yenny Sariasih