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All Journal Jurnal Nukleus Peternakan
Johny N. Kihe
Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Veterinary

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PENGARUH LAMA EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS-MODIFIKASI DENGAN PENAMBAHAN KRIOPROTEKTAN (Effect of equilibration time on frozen sement quality of the duroc pig in tris diluents modification With..... Biqshierz V.W Koelima; Henderiana L.L. Belli; Johny N. Kihe; Wilmientje M. Nalley
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.5490

Abstract

The purpose of this study was to test the influence of the equilibration time on the frozen semen quality of duroc pig in modified tris-egg yolk with the addition of extracellular cryoprotectants. Semen is collected twice per week using massage methods from two duroc males aged ±2.5 years which in good health and have been trained in semen colection. The design used is a completely randomized design with 4 tests and 3 treatments T1: equilibration time of 1 hour, T2: equilibration time of 2 hours, T3: equilibration time of 3 hours at a temperature of 50C. Observed parameters were motility, viability, abnormality, MPU, and recovery rate (RR). The results showed that motility, viability, abnormalities, MPU, and RR were higher (P<0.05) at 2 hours of equilibration (25.63±3.15%, 55.86±5.41%, 4.92±0.53%, 56.69±5.64%, 36.61±4.49%) compared to 1 and 3 hours (20.00±3.54%, 39.28±9.89%, 7.93±0.76%, 40.21±9.39%, 28.57±5.05%) and (15.00±4.08%, 32.19±7.0%, 8.41±0.70%, 32.71±7.07%, 21.43±5.83%). It was concluded the motility of duroc pig spermatozoa in the tris-modified diluents after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the requirement of 40% spermatozoa motility for artificial insemination. It was concluded that the sperm motility of Duroc pig in tris-modified diluent after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the standard of 40% spermatozoa motility for artificial insemination.
PENGARUH SUBTITUSI SARI BUAH SEMANGKA (Citrullus lanatus) DALAM PENGENCER SITRAT- KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI (Effect of watermelon juice supplementation in citrate - egg yolk extender on spermatozoa quality of bali bulls) Maria M. Bria; Wilmientje M. Nalley; Johny N. Kihe; Thomas M. Hine
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.4393

Abstract

This study aims to determine the effect of adding watermelon juice (WJ) into citrate-egg yolk (C-EY) diluents on the spermatozoa quality of bali catlle. Semen was collected twice a week using an artificial vaginal method of three bali bulls with normal body condition and reproductive organs. Semen that has motility > 70%, concentration >1000 x 106 and abnormalities ≤ 20 % was used in this study. Semen then divided into five treatments: C-EY 100% (T0), C-EY-25% WJ (T1), C-EY-50% WJ (T2), C-EY-75% WJ (T3), WJ 100% (T4) and then stored in a refrigerator at temperature 3-5oC. The quality of spermatozoa was evaluated every 24 hours for sperm motility, viability, and abnormalities. The results showed that spermatozoa diluted with S-KT supplemented with 75% WJ (T3) had a higher quality (P<0,05) compared to the other treatments, with motility 40,53 %, viability 47,35%, and abnormalities 4,21% on the 6th day of preservation. It was concluded that the addition of WJ to C-EY diluent was able to maintain the quality of bali bull spermatozoa, with the best SBS concentration is 75%.
Co-Authors Biqshierz V.W Koelima Henderiana Belli Maria M. Bria Wilmientje Marlene Mesang Nalley