Jurnal Nukleus Peternakan
Vol 9 No 1 (2022): Juni

PENGARUH SUBTITUSI SARI BUAH SEMANGKA (Citrullus lanatus) DALAM PENGENCER SITRAT- KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI (Effect of watermelon juice supplementation in citrate - egg yolk extender on spermatozoa quality of bali bulls)

Maria M. Bria (Universitas Nusa Cendana)
Wilmientje M. Nalley (Universitas Nusa Cendana)
Johny N. Kihe (Universitas Nusa Cendana)
Thomas M. Hine (Universitas Nusa Cendana)

Article Info

Publish Date
30 Jun 2022


This study aims to determine the effect of adding watermelon juice (WJ) into citrate-egg yolk (C-EY) diluents on the spermatozoa quality of bali catlle. Semen was collected twice a week using an artificial vaginal method of three bali bulls with normal body condition and reproductive organs. Semen that has motility > 70%, concentration >1000 x 106 and abnormalities ≤ 20 % was used in this study. Semen then divided into five treatments: C-EY 100% (T0), C-EY-25% WJ (T1), C-EY-50% WJ (T2), C-EY-75% WJ (T3), WJ 100% (T4) and then stored in a refrigerator at temperature 3-5oC. The quality of spermatozoa was evaluated every 24 hours for sperm motility, viability, and abnormalities. The results showed that spermatozoa diluted with S-KT supplemented with 75% WJ (T3) had a higher quality (P<0,05) compared to the other treatments, with motility 40,53 %, viability 47,35%, and abnormalities 4,21% on the 6th day of preservation. It was concluded that the addition of WJ to C-EY diluent was able to maintain the quality of bali bull spermatozoa, with the best SBS concentration is 75%.

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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Veterinary


Aims Jurnal Nukleus Peternakan purposes to publish original research and reviews articles on tropical veterinary medicine and domesticated animals such as dog, cat, cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as Indonesian wild life. Scope Jurnal Nukleus Peternakan cover a broad ...