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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Samakia: Jurnal Ilmu Perikanan
Agustiani, Rina Dwi
International Women University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Other

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Quantity of Coliform Bacteria as Bioindicator of Water Pollution (Case Study: Several Tributaries in Purwokerto City, Banyumas Regency, Central Java) Rahayu, Nur Laila; Hendarto, Eko; Sulistiyawati, Indah; Agustiani, Rina Dwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 5, No 1 (2020): February 2020
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (536.91 KB) | DOI: 10.24002/biota.v5i1.2938

Abstract

The city of Purwokerto is crossed by several large rivers, this research examines several tributaries of a large river. Some tributaries that pass through the city of Purwokerto are the Raden River, the Caban River, the Jurig River and the Luhur River. Tributaries have the potential to influence water quality. This study aimed to analyze the quantity of coliform bacteria as an indicator of water pollution in several tributaries in Purwokerto City, Banyumas Regency. Microbiological tests were carried out by calculating the total coliform quantity and fecal coliform using the Most Probable Number (MPN) test. Total coliform and fecal coliform can be used as a reference indicator of water quality in the presence of water pollution. The laboratory test results for the quantity of coliform bacteria showed in several tributaries in Purwokerto City, Banyumas Regency, very high in total coliform or fecal coliform, exceeding the water quality standard.
DNA Barcoding Ikan Kepala Timah Dan Betok Berdasarkan Gen COI sebagai Ikan Pioneer di Kolong Pascatambang Timah, Pulau Bangka Diah Mustikasari; Rina Dwi Agustiani
Samakia : Jurnal Ilmu Perikanan Vol 12 No 1 (2021): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.817 KB) | DOI: 10.35316/jsapi.v12i1.1005

Abstract

Tin mining activities in the Bangka Belitung Archipelago Province have led to the formation of pits that have not ideal water quality for aquatic organisms in general. Acidic pH and high metal contamination at certain age under certain causes only a few species are able to live well in these ecosystems. This study aimed to determine the identity of the pioneer fishes in ex-tin mining pit <1 year of Bangka Island through DNA barcoding based on COI gene. The pioneer fish in ex-tin mining pit <1 year were Kepala Timah fish dan Betok fish. Species determination was based on sequences similarity in NCBI and BOLD System showed that Ikan Kepala Timah had similarity with Aplocheilus panchax and its morphology indicated Ikan Kepala Timah was Aplocheilus panchax. The sequences similarity of Betok fish in NCBI and BOLD System showed that Betok fish had similarity with Anabas testudineusi and its morphology showed that Betok fish was Anabas testudineusi. The Kimua 2 Parameters (K-2P) Neigh­bour-Joining (NJ) clearly confirmed that Kepala Timah fish found in ex-tin mining pit <1 year was Aplocheilus panchax, whereas Betok fish was located outside clade of the group. It indicated that Betok fish was found in ex-tin mining pit <1 year can be expected as a morphotype of Anabas testudineusi that needed further confirmation in other studies, both molecularly and morphologically. The sequence data of COI gene of Betok fish found in ex-tin mining pit <1 year was expected can supported available sequence data of Anabas testudineusi in NCBI and BOLD System.
Co-Authors Diah Mustikasari Eko Hendarto Rahayu, Nur Laila Sulistiyawati, Indah