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Quantity of Coliform Bacteria as Bioindicator of Water Pollution (Case Study: Several Tributaries in Purwokerto City, Banyumas Regency, Central Java) Rahayu, Nur Laila; Hendarto, Eko; Sulistiyawati, Indah; Agustiani, Rina Dwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 5, No 1 (2020): February 2020
Publisher : Universitas Atma Jaya Yogyakarta

The city of Purwokerto is crossed by several large rivers, this research examines several tributaries of a large river. Some tributaries that pass through the city of Purwokerto are the Raden River, the Caban River, the Jurig River and the Luhur River. Tributaries have the potential to influence water quality. This study aimed to analyze the quantity of coliform bacteria as an indicator of water pollution in several tributaries in Purwokerto City, Banyumas Regency. Microbiological tests were carried out by calculating the total coliform quantity and fecal coliform using the Most Probable Number (MPN) test. Total coliform and fecal coliform can be used as a reference indicator of water quality in the presence of water pollution. The laboratory test results for the quantity of coliform bacteria showed in several tributaries in Purwokerto City, Banyumas Regency, very high in total coliform or fecal coliform, exceeding the water quality standard.

Uji Ekstrak Bawang Putih Tunggal (Allium sativum L.) Terhadap Bakteriuria Escherichia coli Pada Calon Pekerja Migran Indonesia Indah Sulistiyawati; Muhammad Falah; Ari Dwi Nurasih
Biotropic : The Journal of Tropical Biology Vol. 5 No. 1 (2021): Biotropic, Volume 5 Nomor 1, 2021
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2021.5.1.50-57

Garlic (Allium sativum L) is a tested type of phytopharmaca because it is proven to have beneficial benefits as antibacterial and protect the body from pathogens. The majority of Indonesian Migrant Workers, especially Prospective Indonesian Migrant Workers (CPMI), are indicated to be susceptible to Urinary Tract Infection (UTI), which is caused by the bacteriuria Escherichia coli. In this study, the crude extract of single garlic (A. sativum L) will be used as an antibacterial against E. coli bateriuria in the urine sample of CPMI, with variations in the concentration of single crude extract of garlic, namely; 0%, 25%, 50%, 75%, and 100%. This research was conducted in a period of 1 (one) year from January to December 2020, with urine research samples from CPMI in the Banyumas Regency area. Extraction was carried out by extracting a single garlic using 96% ethanol solvent in a ratio of 1: 1, then evaporated to produce a filtrate that would be tested with a varied concentrate composition. Isolation of bacteriuria in urine samples using EMBA selective media. From a total of 37 urine samples, 17 about 46% were positive for E. coli, and 20 samples were 54% negative for E. coli. Bacteriuria that was found challenged with crude garlic extract resulted in differences in the antibacterial test at several extract concentrations with amoxicillin positive control, with One Way Anova a P-value = 0.000 <0.05, and continued with the analysis of the Post Hoc Test and the Tuckey Test, that the garlic extract The single most effective used as an anti-bacterial agent is the concentration of 100%, resulting in an average inhibition of 12.81 mm. The ability of single garlic extract to inhibit increases with increasing extract concentrate.

Kuantitas Total Bakteri Coliform pada Instalasi Pengolahan Limbah Cair Medis Laboratorium Klinik Indah Sulistiyawati
Jurnal Ilmiah Universitas Batanghari Jambi Vol 19, No 3 (2019): Oktober
Publisher : Universitas Batanghari Jambi

Health service activities at the Clinical Laboratory produce infectious and non-infectious waste in the form of solid or liquid. Clinical laboratory liquid waste is one source of environmental pollution if the concentration exceeds the standard quality of wastewater. Polluted water quality parameters include microbiologics of Coli / Total Coliform Germs. Water quality with microbiological parameters can be used to determine the presence of bacteria, viruses, parasites. Bacteria used as indicators are Coliform bacteria. At present several clinical laboratories have implemented a Liquid Wastewater Treatment Plant. So that the waste discharged into the environment meets the standard quality standards for hospital liquid waste, and minimal pollution. This study aims to determine the quantity of Total Coliform at the inlet and outlet points of the Wastewater Treatment Plant Laboratory Medical Liquid. The results showed that the quantity of Total Coliform at the inlet sampling point of the liquid media waste at the Wastewater Treatment Plant Laboratory reached the highest value > 1.6 x 105 MPN / 100 ml, and after processing at the Wastewater Treatment Plant Laboratory decreased to 0 MPN / 100 ml (no coliform was found ), and with the highest value of 2.7 x 103 MPN / 100 ml, according to the waste water quality standard.

Profil Ekspresi MikroRNA Kanker Payudara di Purwokerto Ari Dwi Nurasih; Indah Sulistiyawati; Muhammad Falah
Jurnal Kesehatan Andalas Vol 9, No 4 (2020): Online December 2020
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i4.1553

Penemuan metode deteksi dini kanker payudara secara sensitif, spesifik dan minimal invasif merupakan kunci dalam keberhasilan pengobatan. Hal ini dapat dilakukan menggunakan biomarker yang bersirkulasi dalam plasma darah yaitu mikroRNA. MikroRNA adalah asam ribonukleat yang tidak mengkode protein tetapi memiliki peran dalam menghambat dan mendegradasi mRNA sehingga mempengaruhi perkembangan kanker. Tujuan: Mengetahui profil ekspresi mikroRNA pada pasien kanker payudara di Purwokerto dan mikroRNA yang mengalami disregulasi sebagai kandidat biomarker kanker payudara. Metode: Analisis profil ekspresi mikroRNA pada kanker payudara dilakukan dengan isolasi plasma dari pasien kanker payudara dan individu sehat, sintesis cDNA, dan kuantifikasi mikroRNA dengan quantitative real time PCR (qPCR). Kemudian dilakukan penghitungan dengan metode Livak untuk mengetahui mikroRNA yang mengalami peningkatan dan penurunan ekspresi yang signifikan. Hasil: Biomarker kanker payudara di Purwokerto adalah MikroRNA yang mengalami upregulation adalah hsa-miR-543 (sebanyak 1612,28 kali), hsa-miR-495-3p (1797,95 kali), hsa-miR-382-5p (8780,32 kali), hsa-miR-155-5p (8552,18 kali) dan hsa-miR-154-5p (2339,41 kali). mikroRNA yang mengalami downregulation adalah hsa-miR-590-5p (sebanyak 4,70 kali), hsa-miR-33a-5p (sebanyak 74,29 kali), hsa-miR-100-5p (sebanyak 23,09 kali), hsa-miR-19b-3p (sebanyak 11,71 kali) dan hsa-miR-144-3p (sebanyak 25,30 kali) (p < 0,05). Simpulan: Biomarker yang dapat digunakan sebagai alat deteksi dan diagnostik dini kanker payudara di Purwokerto adalah miR-382-5p.Kata kunci: biomarker, kanker payudara, mikroRNA, qPCR

Quantity of Coliform Bacteria as Bioindicator of Water Pollution (Case Study: Several Tributaries in Purwokerto City, Banyumas Regency, Central Java) Nur Laila Rahayu; Eko Hendarto; Indah Sulistiyawati; Rina Dwi Agustiani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 5, No 1 (2020): February 2020
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v5i1.2938

The city of Purwokerto is crossed by several large rivers, this research examines several tributaries of a large river. Some tributaries that pass through the city of Purwokerto are the Raden River, the Caban River, the Jurig River and the Luhur River. Tributaries have the potential to influence water quality. This study aimed to analyze the quantity of coliform bacteria as an indicator of water pollution in several tributaries in Purwokerto City, Banyumas Regency. Microbiological tests were carried out by calculating the total coliform quantity and fecal coliform using the Most Probable Number (MPN) test. Total coliform and fecal coliform can be used as a reference indicator of water quality in the presence of water pollution. The laboratory test results for the quantity of coliform bacteria showed in several tributaries in Purwokerto City, Banyumas Regency, very high in total coliform or fecal coliform, exceeding the water quality standard.

PENERAPAN STRATEGI PEMBELAJARAN PROBLEM BASED LEARNING MELALUI LESSON STUDY UNTUK MENINGKATKAN KETERAMPILAN MEMECAHKAN MASALAH MAHASISWA Zainul Mustofa; Anna Jarrotul Khoiriyah; Indah Sulistiyawati; Diah Harmawati; Mimien Heni Irawati Al Muhdhar
Jurnal Pendidikan Biologi Vol 8, No 1 (2016)
Publisher : Universitas Negeri Malang

This research aims at improving students' problem solving skills. The type of research used is Classroom Action Research by implementing Lesson Study-based Problem Based Learning strategy as an effort to improve students’ problem solving skills. This study was conducted for 1 cycle consisting of two meetings. The results of this research show that LS-based PBL strategy can improve students' problem solving skills, from 30% during observation to 93%. The data of students' average problem solving skill scores reached 80.07. The achievement of this score has met the minimum standard score of State University of Malang, which is 55.00. Based on these results, learning by using LS-based PBL strategy can improve students' problem solving skills.

Deteksi Resistensi Amoxicillin Helicobacter pylori pada Pasien Dispepsia Indah Sulistiyawati; I Gede Arinton; Hendro Pramono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.308

Amoxicillin is one of the antibiotics that commonly used on treatment of H. pylori infection. pbp1A gene mutation in H. pylori is a dominant cause of amoxicillin resistance. This study was aimed to evaluate the presence of H. pylori in patients with dyspepsia by using non-invasive method i.e. H. pylori stool antigen (HPSA) and invasive method i.e. pbp1A gene amplification, and also evaluate the amoxicillin resistance of H. pylori by assessing the pbp1A gene mutations. The samples were 26 faeces and 26 gastric biopsies of patients with dyspepsia from the Internal Disease of Prof. Dr. Margono Soekardjo Hospital in Purwokerto. DNA amplification performed by using polymerase chain reaction (PCR) to detect the presence of amoxicillin resistance encoding gene i.e. penicillin binding protein (pbp1A). Sequencing of the DNA sample was performed at the First Base Malaysian Company, to analyze the existence of a point mutation. DNA sequencing analysis of 12 samples showed the presence of a mutations in pbp1A gene from 2 samples, in the third motive of pbp i.e. amino acid changes, Alanine 599 substituted to Threonin and Threonin 592 to Alanine. Those mutations become a dominant risk factor for resistance of H. pylori, toward the bacterial peptidoglycan synthesis. In this research, it was known that the detection of H. pylori infection by using PCR remains more accurate and specific method. The presence of H. pylori mutant strains in this study may becomes the risk factors of resistance to amoxicillin treatment.

Uji Sinergitas Rendaman Tembakau (Nicotiana tabacum L.) dengan Jamur Trichoderma spp. Secara In Vitro dan Potensinya Sebagai Gabungan Biopestisida Alami Nura Khabita; Indah Sulistiyawati; Ari Dwi Nurasih
Jurnal Ilmiah Universitas Batanghari Jambi Vol 22, No 2 (2022): Juli
Publisher : Universitas Batanghari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/jiubj.v22i2.2263

Management of plant pests and diseases can prevent crop failure of agricultural commodities. Trichoderma spp. is a type of biological control fungus. Tobacco can be used as a vegetable pesticide. However, the synergy of tobacco and Trichoderma spp. when applied simultaneously in controlling pathogens is not yet known, considering the content of the active compound nicotine in tobacco which has antifungal properties, it is necessary to conduct this study with the aim of knowing the effect of tobacco immersion on the growth of the fungus Trichoderma spp. and if there is no inhibition then both will have the potential as a combination of natural biopesticides. This study used a completely randomized design (CRD). The treatment tried was different concentrations of tobacco soak mixed with PDA as a culture medium for Trichoderma spp. The concentrations tested were 0% (T0, positive control), 5% (T1), 10% (T2), 15% (T3), and 20% (T4). The negative control used a fungicide with the active ingredient mankozeb. Each treatment was repeated 3 times. Colony thickness and color data were observed visually by descriptive method. Meanwhile, data on colony diameter, number of spores, dry weight of mycelium were analyzed by F test, if significantly different, then followed by DMRT test with a confidence level of 5%. Based on research that has been carried out from July-August 2021, the results obtained on PDA media with tobacco soaking content with concentrations of 0% (K+), 5% (T1), 10% (T2), 15% (T3), 20% (T4 ) can be grown with Trichoderma spp., while the media with the addition of synthetic fungicides made from mankozeb is not grown. The difference in concentration showed different growth results and the effective concentration in this research was 15%, because at 20% concentration there was a decrease in spore density and mycelium dry weight.

PEMANFAATAN SERBUK GERGAJI KAYU UNTUK BUDIDAYA JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS) DI KELOMPOK MASYARAKAT DESA PASIR KIDUL Eti Wahyuningsih; Indah Sulistiyawati; Nur Laila Rahayu
Diseminasi: Jurnal Pengabdian kepada Masyarakat Vol. 4 No. 2 (2022)
Publisher : Pusat Pengabdian kepada Masyarakat- LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/diseminasiabdimas.v4i2.2704

Pasir Kidul Village is one of the industrial villages in West Purwokerto District, Banyumas. Wood industrial waste (pencil factory), namely wood sawdust and wood chips accumulate and disturb the surrounding environment. One of the uses of this waste is the raw material for white mushroom baglog (Pleurotus ostreatus). This Community Partnership Program (PKM) aims to utilize wood sawdust for the cultivation of white oyster mushroom (Pleurotus ostreatus) in community groups in Pasir Kidul Village, West Purwokerto District, Banyumas Regency. This PKM is held for 5 months, from August to December 2018. There are 19 community groups in this community partnership program, with the target that after counseling, training and assistance in the application of "white oyster mushroom cultivation" technology can be applied to 80% of the program participants. The extension stages were training on how to make lemur and baglog for white oyster mushroom cultivation, training on planting seeds and maintaining white oyster mushroom cultivation media, counseling on the influence of types of growing media on white oyster mushroom production, and counseling on environmental factors as determinants of the success of white oyster mushroom cultivation. Some of the problems found by farmers who have carried out cultivation are Baglog sterilization and baglog rot. The results of the community partnership program show that there is an optimization of the utilization of wood sawdust waste which is abundant in the village, by processing wood sawdust into a growing medium for white oyster mushrooms (Pleurotus ostreatus) so that it has a higher useful value and selling value. Pasir Kidul adalah Kelurahan di Kecamatan Purwokerto Barat, Kabupaten Banyumas, Provinsi Jawa Tengah, Indonesia. Desa Pasir Kidul merupakan salah satu desa industri dengan terdapatnya industri pengolahan kayu (pabrik pensil) yang berdampak langsung dengan melimpahnya limbah padat berupa serbuk gergaji kayu dan serpihan kayu yang terbuang menumpuk di suatu lokasi tertentu yang dapat mengganggu kondisi lingkungan sekitar. Selama ini, serbuk gergaji kayu dibiarkan membusuk, ditumpuk dan dibakar yang berdampak negatif terhadap lingkungan sehingga penanggulangannya perlu dipikirkan. Salah satu upaya untuk membantu mengatasi permasalahan menumpuknya serbuk gergaji kayu di Desa Pasir Kidul adalah pemanfaatan serbuk gergaji kayu untuk budidaya jamur tiram putih (Pleurotus ostreatus) di kelompok masyarakat Desa Pasir Kidul Kecamatan Purwokerto Barat Kabupaten Banyumas. Upaya daur ulang serbuk gergaji kayu yaitu dengan memanfaatkan serbuk gergaji kayu tersebut untuk media tanam jamur tiram putih (Pleurotus ostreatus). Menurut Rachman dan Malik (2011), jika dilihat dari mata rantai industri pengolahan kayu, maka dalam industri penggergajian terjadi proses perubahan kayu pertama kali yaitu kayu dalam bentuk dolok menjadi kayu gergajian (sawn timber) atau disebut juga kayu konversi berupa papan, balok, tiang dan sortimen lainnya.

Budidaya Empon-Empon dan Praktik Pembuatan Jamu pada Siswa Sekolah Menengah Pertama Indah Sulistiyawati; Wahyu Maulana Endris; Chamid Sutikno
Madaniya Vol. 3 No. 4 (2022)
Publisher : Pusat Studi Bahasa dan Publikasi Ilmiah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53696/27214834.295

Kegiatan budidaya empon-empon di Kabupaten Banyumas sudah menjadi tradisi budaya dalam bentuk kearifan lokal, yang didefinisikan sebagai strategi kehidupan yang berwujud aktivitas hidup. Seiring perkembangan jaman tradisi budidya empon-empon dan pembuatan jamu tradisional mulai tidak dikenali generasi muda, olehkarenya diperlukan upaya pelestarian. Tujuan program ini mengenalkan dan melatih ketrampilan hardskill siswa SMP Maarif NU 1 Purwokerto dalam budidaya empon-empon dan membuat jamu tradisional. Metode yang digunakan dalam pelaksanaan kegiatan meliputi; koordinasi program, sosialisasi, penyuluhan, dan pelatihan serta evaluasi dan monitoring pelaksanaan kegiatan. Kegiatan ini telah diikuti sejumlah 32 siswa kelas IX SMP Maarif NU 1 Purwokerto, yang diawali dengan kegiatan pre test untuk mengukur pemahaman materi dan dilanjutkan penyuluhan dan praktik budidaya dan membuat jamu tradisional, serta diakhiri dengan post test. Peningkatan hasil pre test dan post test menjadi tolok ukur kelancaran dan keberhasilan program dengan peningkatan 25%. Selama kegiatan berlangsung mitra sangat antusias mengikuti dan telah memiliki perubahan dengan kemampuan mempraktekkan ketrampilan buddidaya empon-empon dan membuat jamu tardisional sebagai bentuk pelestarian lingkungan.

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