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All Journal Jurnal Kesehatan Masyarakat Indonesian Journal of Biotechnology Majalah Obat Tradisional JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) JFIOnline Jurnal Formil (Forum Ilmiah) Kesmas Respati
Susi Iravati
Faculty of Medicine, Universitas Gadjah Mada. Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : Indonesian Journal of Biotechnology

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Detection of eae, bfpA, espA Genes on Diarrhoeagenic Strains of Escherichia coli Isolates Harti, Agnes Sri; Iravati, Susi; Asmara, Widya
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

The Enteropathogenic Escherichia coli (EPEC) is one of pathogenic strain of diarrheagenic E. coli group in children andinfant that occurs in developing countries. The significant virulence factors in pathogenic EPEC are eaeA (E. coli attachingeffacing), bfpA (bundle-forming pilus A) and espA (encoding secreted protein A) genes. The use of DNA probes to detect thevirulence genes in E. coli in Indonesia is not common yet. In this experiment the gene fragments of eae, bfpA, and espA were usedas probes to detect the EPEC among E. coli isolates from stool specimensin of diarrheic children attending Public Health Centersin Yogyakarta. The DNA samples were isolated from 49 diarrheagenic E. coli isolates. The DNA probes of eae, bfpA and espAwere obtained by amplification of DNA fragment of EPEC O126 using PCR technique. Furthermore, those probes were used toidentify the presence of those genes among E. coli isolates using hybridization technique. The results showed that 42 (85.7%)isolates were espA+, 25 isolates (51%) were eaeA+ (EPEC strains). Therefore among 25 isolates of EPEC, 20 isolates (80 %)among EPEC were bfpA+ (typical EPEC strains).Keywords : DNA probe, eae, bfpA, espA, EPEC.
Co-Authors Adi Heru Sutomo Agnes Sri Harti Ardiansyah, Ririn Teguh Ariyanto Nugroho Damar Tri Boewono Dwi Astuti Eni Harmayani Erna Prawita Setyowati Fauzia Nur Laili, Fauzia Nur Lily Arsanti Lestari Peni Indrayudha Sarto Soesatyo, Marsetyawan Heparis Nur Ekandaru Widya Asmara Wijaya, Abdul Rosyid Thoyib Wijaya, Abdul Rosyid Thoyib Yulia Rina Wijaya