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OPTIMASI PRODUKSI ENZIMATIS DIASILGLISEROL MELALUI GLISEROLISIS KONTINU [Optimization of Enzymatic Diacylglycerol Production through Continuous Glycerolysis] - Tri-Panji; Septiany C. Palilingan; I Made Artika
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

OPTIMASI PRODUKSI ENZIMATIS DIASILGLISEROL MELALUI GLISEROLISIS KONTINU[Optimization of Enzymatic Diacylglycerol Production through Continuous Glycerolysis]Tri-Panji1)*, Septiany C. Palilingan2) dan I Made Artika2)1) Balai Penelitian Bioteknologi Perkebunan Indonesia, Jl. Taman Kencana No. 1 Bogor2) Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 24 Juni 2013 / Disetujui 10 Februari 2014ABSTRACT Diacylglycerol (DAG) produced from crude palm oil (CPO) is one of the healthy oils that can be consumed for daily human diet. DAG production in Indonesia is constrained by the high cost of the mostly imported lipase. To overcome this problem, research of DAG production has been carried out using crude extracts of lipase produced by local species of fungi Rhizopus oryzae. This study aims to develop a continuous process of enzymatic glycerolysis of CPO for DAG production; to establish optimum conditions of DAG production which includes flow rate of CPO and glycerolysis time; and to test the performance of lipase from the local mold R. oryzae in catalyzing continuous process of glycerolysis for the production of DAG. Lipase isolation was carried out by acetone precipitation and lipase was used as a catalyst in the continuous glycerolysis process. The glycerolysis was conducted by reacting CPO with glycerol continuously at various time periods. The optimum condition of automatic continuous glycerolysis process was achieved at a CPO flow rate of 3 mL/min with a glycerolysis time at the 18 cycles (9 hours). The conversion of DAG was 29%. The performance of lipase was proven to remain stable up to 3 times changes of CPO substrate for 9 hours of glycerolysis process with the best condition at the 3 cycles and can improved conversion of DAG until 37%.

Penggunaan kombinasi adsorben sebagai media filtrasi dalam menurunkan kadar fosfat dan amonia air limbah laundry Septiany Christin Palilingan; Meity Pungus; Farly Tumimomor
Jurnal Kimia Fullerene Vol 4 No 2 (2019): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Wastewater of detergent residual that comes from the washing process (laundry) has the potential to pollute environmental sustainability such as rivers and soil if it is thrown away in large volumes without any prior processing. Easy and inexpensive methods for processing laundry wastewater for household-scale have been carried out and proven to reduce levels of pollutants contained in laundry wastewater such as phosphate and ammonia. This study aims to determine the effect of the use of filtration media using a combination of activated charcoal adsorbent, zeolite, silica sand, anthracite and ferolite in reducing phosphate and ammonia levels in laundry wastewater. Based on the results of laboratory tests on the parameters of phosphate and ammonia test, it was found that after the filtration process, there was a decrease in phosphate and ammonia levels in the samples of laundry wastewater by 83.3% and 63.6% respectively. Thus, from the research data, it can be concluded that the filtration media in the form of a combination of adsorbents used in this study proved to be able to reduce phosphate and ammonia levels in samples of laundry wastewater.

Produksi enzimatis Virgin Coconut Oil (VCO) dengan enzim bromelin serta pemurniannya menggunakan adsorben zeolit Septiany Christin Palilingan; Meity Pungus
Jurnal Kimia Fullerene Vol 3 No 2 (2018): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

One of the processed coconut products that is beneficial to health and has been widely used as an industrial raw material is VCO. Traditional rural communities and households have produced their own VCO for personal consumption because of its health benefits. However, the production process of VCO which still traditionally produces VCO products with a standard of quality and yield that has not been maximized. The research was carried out by enzymatic method, which added the enzyme bromelain contained in pineapple stem extract into coconut milk. The VCO product obtained was then purified by zeolite adsorbent, and water content and free fatty acid levels were tested. The results showed the highest VCO yield was found at a concentration of 20%, which was 36% and the purification of VCO with adsorbent zeolite was proven to reduce water content and free fatty acid levels with the highest percentage decrease in water content by 66%, and the highest percentage decrease in free fatty acid 63%. From the results of the study it can be concluded that the addition of pineapple stem extract containing the enzyme bromelain in the production of VCO can increase the yield of VCO to a maximum of 35.9% and purification of VCO samples with adsorbent zeolite proven to help reduce water content and free fatty acid levels.

Pemanfaatan karbon aktif dari sabut kelapa sebagai elektroda superkapasitor Farly Reynol Tumimomor; Septiany Christin Palilingan
Jurnal Kimia Fullerene Vol 3 No 1 (2018): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

In this study, coconut fiber based activated carbon has been used for fabrication of supercapacitors. Iodine absorption test is performed to measured absorption level porous activated carbon sample size is relatively small (microporous). Characteristics of the activated carbon material covering the surface morphology and structure were tested using SEM and XRD. Electrode materials with composition (Activated charcoal: PVDF = 9: 1 (w / w)), the current collector and separator has been assembled to be tested its performance as an electrical charge storage device. The test results by cyclic voltammetry method was to look at the performance supercapacitor devices at once to obtain the value of the capacitance curve obtained voltammograms. Based on the results of the calculation of the capacitance, the highest capacitance values obtained in the supercapacitor with activated carbon electrodes in coconut fiber steam 50 mL / bar with a capacitance value is 50.73 F / g.

Penurunan kadar BOD dan COD dalam limbah cair laundry menggunakan kombinasi adsorben alam sebagai media filtrasi Meity Pungus; Septiany Christin Palilingan; Farly Tumimomor
Jurnal Kimia Fullerene Vol 4 No 2 (2019): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.872 KB)

Laundry wastewater is generated in large quantities will pollute the environment such as rivers and soil if thrown away without any prior treatment. Laundry wastewater treatment methods that have been carried out are easy and inexpensive for household scale and have been proven to reduce levels of organic pollutants. Two parameters that can indicate the presence of organic pollutants in laundry wastewater are Biological Oxygen Demand (BOD) and Chemical Oxygen Demand (COD). This study aims to determine the effect of the use of filtration media in the form of a combination of activated carbon adsorbent, zeolite, silica sand, anthracite and ferolite in reducing levels of BOD and COD in laundry wastewater. Based on the results of laboratory tests on the BOD and COD test parameters, it was found that after the filtration process there was a decrease in the BOD and COD levels in the laundry wastewater samples respectively by 53% and 54%. The results of the statistical analysis also showed that the presence of filtration treatment had a significant effect in reducing levels of BOD and COD in laundry wastewater samples. Thus, it can be concluded that the presence of filtration treatment is proven to significantly reduce BOD and COD levels which can also indicate decreased levels of organic pollutants contained in laundry wastewater.

Pengaruh Model Pembelajaran Cooperative Learning Tipe Jigsaw Terhadap Hasil Belajar Siswa Di SMA Negeri 6 Halmahera Utara Yosina Sylena Souhuwat; Septiany Ch Palilingan
Oxygenius Journal Of Chemistry Education Vol 3 No 2 (2021): Oxygenius: Journal of Chemistry Education
Publisher : Chemistry Department, Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/ojce.v3i2.290

The purpose of this study was to determine the difference in the average student learning outcomes of classes taught with the jigsaw cooperative learning model and the average learning outcomes of conventional models. This research is a quantitative study using a non-equivalent control group design. The population in this study were all students of class XI MIPA SMA Negeri 6 North Halmahera, the sample consisted of 2 classes, namely XI MIPA 3 as the control class and XI MIPA 4 as the experimental class. Research data obtained in the form of the difference in the average value of the pretest and posttest, the experimental class of 29.7, and the difference in the average value of the pretest and posttest of the control class of 17.95. After being tested through the t-test, the results obtained between the control class and the experimental class were significant, namely = 2,024 Thus, it can be concluded that there is a difference in the average learning outcomes of classes that are applied using the jigsaw type of cooperative learning model with classes that use conventional models.

Pengaruh Metode Problem Based Learning Pada Materi Redoks Di Kelas X SMA Negeri 1 Wori Regina Mongi; Meytij J Rampe; Septiany Ch Palilingan
Oxygenius Journal Of Chemistry Education Vol 1 No 2 (2019): Oxygenius : Journal of Chemistry Education
Publisher : Chemistry Department, Universitas Negeri Manado

The purpose of this study, to determine whether there are differences in student learning outcomes in using the Problem Based Learning method with the lecture method. During approximately 1 month different methods were applied in 2 classes, namely the Problem Based Learning class and the Lecture class with a total of 25 students each, then to see the learning outcomes a post-test was conducted. The results showed the value of t arithmetic = 2.92> t table 2.010, so that the conclusion Ha is accepted and H0 is rejected, it means there are differences in student learning outcomes using the Problem Based Learning method with those using the lecture method on redox material.

Penerapan Media Pembelajaran Berbasis Video Animasi Pada Materi Laju Reaksi dan Faktor-faktor Yang mempengaruhi Laju Reaksi Adelia Mewengkang; Meytij J Rampe; Septiany Ch Palilingan
Oxygenius Journal Of Chemistry Education Vol 2 No 1 (2020): Oxygenius : Journal of Chemistry Education
Publisher : Chemistry Department, Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/ojce.v2i1.143

The Purpose the Study Determine The Average Students Learning on Animation Video Media. The research is a experimental class XI MIA with one class experimental and one class control each class consisting of 30 students. Data the results a average 86,40 for experimental average 53,50 for class control. This matter average class experimental higher then class control.

Pengaruh Metode Demonstrasi Terhadap Hasil Belajar Siswa Pada Materi Sistem Koloid Ijing Modeong; Desire A. S. Rumondor; Septiany Ch. Palilingan
Oxygenius Journal Of Chemistry Education Vol 4 No 1 (2022): Oxygenius : Journal of Chemistry Education
Publisher : Chemistry Department, Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/ojce.v4i1.350

The demonstration method is a method that can make students' attention more focused on what is being taught so that students are more active and do not feel bored in learning. This study aims to see the effect of the demonstration method on student chemistry learning outcomes on colloidal system material at SMA Negeri 1 Tutuyan in the 2020/2021 academic year. This research is a pre-experimental research with one group pretest-posttest design. The population in the study were all students of class XI IPA and the sample taken was class XI IPA MIA 1, totaling 30 people. Testing the hypothesis using the t-test formula at the significant level = 0.05. Based on the data analysis, the value of tcount = 23.90 and ttable = 2.045. The value of tcount > ttable then H1 is accepted and H0 is rejected, so it can be concluded that H1 is accepted, which means there is a significant difference between student learning outcomes on colloidal system material before and after using the demonstration method because there are differences. student learning outcomes. To find out how much the effectiveness of using the demonstration method is continued with the N-Gain test and obtained is 0.76 with high criteria.

Sintesis Diasilgliserol Melalui Gliserolisis Enzimatis Kontinu Menggunakan Enzim Lipase Amobil Septiany Christin Palilingan
Jurnal Kimia Fullerene Vol 7 No 1 (2022): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v7i1.403

Sintesis minyak diasilgliserol (DAG) dari crude palm oil (CPO) masih terkendala mahalnya harga enzim lipase komersial yang masih diimpor dari luar negeri. Telah dilakukan penelitian sintesis DAG menggunakan enzim lipase amobil yang dihasilkan dari kapang lokal Rhizopus oryzae dan diamobilisasi dalam butiran zeolit. Penelitian ini bertujuan untuk menentukan waktu gliserolsis enzimatis kontinu yang optimum dalam sintesis DAG; serta untuk menguji aktivitas dan stabilitas enzim lipase amobil dalam mengkatalisis gliserolisis enzimatis kontinu. Isolasi enzim lipase dilakukan melalui pengendapan dengan aseton serta diamobilisasi pada butiran zeolit. Sintesis DAG melalui gliserolisis enzimatis kontinu dilakukan dengan mengalirkan substrat secara kontinu ke dalam bioreaktor dengan berbagai variasi waktu dan penggantian substrat. Hasil penelitian menunjukkan bahwa DAG berhasil disintesis melalui gliserolisis enzimatis kontinu menggunakan enzim lipase amobil, dengan waktu gliserolisis optimum pada jam ke-21 dengan nilai konversi DAG sebesar 33 %. Adanya penggantian substrat ternyata membuat aktivitas dan stabilitas enzim lipase amobil cenderung semakin menurun.

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