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PENGARUH CaCl2DAN EDIBLE FILM TERHADAP PENGHAMBATAN CHILLING INJURY BUAH NANGKA KUPAS [Effect of CaCl2 and Edible Film on Chilling Injury Inhibition of fresh-cut Jackfruits] Ida Bagus Banyuro Partha; Suparmo .; Moh Ali Joko Wasono; Maria Ulfah
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 1 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.964 KB)

Abstract

Effect of CaCl2 soaking and edible film coating to delay or inhibit chilling injury of freshly-cut jackfruit has been studied. The onset of chilling injury was monitored based on sadden increase of ethylene emission as measured on-line using CO2-laser driven photoacoustic spectrometer.Freshly cut edible part of ripe jackfruit was used as the research material. The fruit was soaked in 0%, 1%, 1,5%, 2%  of CaCl2  for 15 minutes. After rinsing, sample were divided into two parts each for edible coating and control. The edible film was prepared by soaking of the samples for 30 seconds in solution of white bean (Phaseolus lunatus, L.) edible coating, which was derived from our previous research. The samples were devided into two parts for destructive and non destructive analysis. Both were then stored at 4oC for 3 days, at which time the normal fruit suffer from chilling injury. For the non-destructive analysis, the samples were individually put in special cuvet and a flow-trough air system was connected to each cuvet for on-line ethylene monitoring. Data of real-time ethylene emission were collected  automatically every 120 minutes during the three day cold storage. The other set of samples were put in a plascic bag and stored at 4oC for 3 days period. At the end of cold stored, the samples were let to adjust to room temperature for 1 hr before subjected to respiration rate, reducing sugars, total acidity, and texture analysis.Result indicated that soaking with CaCl2 and edible film coating of freshly-cut jackfruit  delayed the on-set of chilling injury as indicated by late increase of ethylene emission. Based on pattern of ethylene emission  during the three day storage and their respiration rate, the best treatment to delay chilling injury of fresly-cut jackfruit was soaking in CaCl2 2%  in combination with edible film coating.
Efektivitas Poliamin terhadap Penghambatan Chilling Injury pada Beberapa Tingkat Kematangan dan Kemasakan Buah Pisang Mas (Musa paradisiaca, L.) Ida Bagus Banyuro Partha; Suparmo Suparmo; Murdijati Gardjito; Moh. Ali Joko Wasono
agriTECH Vol 28, No 1 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1095.703 KB) | DOI: 10.22146/agritech.9780

Abstract

A study on the effectiveness of polyamines on chilling injury inhibition of banana (Musa paradisiaca, L.) cv. Mas was conducted. It was aimed to identify the effectiveness of polyamines (putrescine, spermidine, spermine) on chilling injury at various levels of maturities and ripeness of banana cv. Mas. The present study exploited banana cv. Mas at 85 % of maturity, full ripe, over ripe, and soaking condition in polyamine solutions, i.e. putrescine (put), spermidine (spd), spermine (spm). The fruits were stored at 10 oC for 10 days and daily real time observations for emitted ethylene were conducted using photo-accoustic spectrometer, while espiration rate, reducing sugar content, textures, chilling injury index (necrosis and pitting) were observed every two days at room temperature after the fruits were keep out from cold storage. Result indicated that soaking in polyamines (putrescine, spermidine, spermine) was able to inhibit emitted ethylene, respiration rate, chilling injury index (necrosis and pitting) and textures softness of banana fruits. The most effective polyamines to inhibit chilling injury in banana cv. Mas fruits at any maturaties and ripeness level was putrescine compound. Putrescine was highly effective to inhibit the chilling injury of banana cv. Mas fruits at 85% maturity.ABSTRAKTelah dilakukan penelitian tentang efektivitas poliamin terhadap penghambatan kerusakan suhu dingin (chilling in- jury) pada buah pisang Mas (Musa paradisiaca, L.), dengan tujuan untuk mengetahui efektivitas poliamin (putresin, spermidin, spermin) terhadap penghambatan chilling injury pada beberapa tingkat kematangan dan kemasakan buah pisang Mas. Penelitian ini menggunakan buah pisang Mas tingkat kematangan 85 %, masak penuh, lewat masak dan perendaman dalam larutan poliamin yaitu putresin (put), spermidin (spd), spermin (spm) dengan konsentrasi 1,5 mM selama 8 menit Buah pisang disimpan selama 10 hari pada suhu 10 oC dan dilakukan pengamatan emisi etilen setiap hari secara real time menggunakan spektrometer fotoakustik, sedangkan laju respirasi, indeks chilling injury (nekrosis dan pitting) dan tekstur dilakukan setiap 2 hari pada suhu kamar setelah buah pisang dikeluarkan dari ruang pend- ingin. Hasil penelitian menunjukkan bahwa perendaman buah dalam larutan poliamin (putresin, spermidin, spermin) mampu menghambat laju respirasi, emisi etilen, indeks chilling injury (nekrosis dan pitting), dan pelunakan tekstur buah pisang Mas.  Jenis poliamin yang paling efektif untuk menghambat chilling injury pada buah pisang Mas untuk semua tingkat kematangan dan kemasakan buah adalah senyawa putresin (put), kemudian berikutnya spermidin (spd), spermin (spm). Senyawa putresin sangat efektif menghambat chilling injury buah pisang Mas pada tingkat kematangan85 %.
Performance Characterization of 450 nm Visible Light Based Photoacoustic Imaging for Phantom Imaging of Synthetic Dye Contrast Agents Mahendra Kusuma Nugraha; Moh. Ali Joko Wasono; Mitrayana Mitrayana
INDONESIAN JOURNAL OF APPLIED PHYSICS Vol 12, No 1 (2022): IJAP Volume 12 ISSUE 01 YEAR 2022
Publisher : Department of Physics, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/ijap.v12i1.49179

Abstract

Performance characterization of 450 nm visible light photoacoustic imaging has been carried out through phantom imaging of methylene blue (MB), methyl orange (MO), and methyl red (MR) dye solutions. The phantom was made of a nylon tube with a diameter of 5.0 mm (outside) and 4.6 mm (inside) having a height of 2.0 mm along with a 6×6 cm black galvanized aluminum plate as the background medium. The nylon tube was filled with each type of solution with varying molecular concentrations of 10, 25, 50 and 100 ppm. Twelve (12) phantom objects were imaged in an area of 10×10 cm. The visible absorption peak known from UV-Visible spectroscopy for each type of solution is at 664 nm (methylene blue), 465 nm (methyl orange), and 522 nm (methyl red). It was also known that the amplitude of PA emissions would increase proportionally to the concentration of dye molecules. Overall, methyl orange solutions had the highest photoacoustic emission amplitude distribution. The analysis showed that the ratio of inner diameter (ID) and wall thickness (WT) between the MB and MO phantom images to the original object were 1:0.83 and 1:0.74 (ID) and 1:3 and 1:1.5 (WT), respectively. On the other hand, the ratio of the outer diameter (OD) of the MR phantom image to the original object is 1:1.28. 
Pengembangan Sistem Deteksi Gas CO pada Gas Hembus Manusia Berbasis Spektroskopi ICOS (Halaman 35 s.d. 38) Restu Widiatmono; Julien Mandon; Frans J. M. Harren; Kusminarto -; M. A. Joko Wasono; Mitrayana -
Jurnal Fisika Indonesia Vol 18, No 52 (2014)
Publisher : Department of Physics Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (915.445 KB) | DOI: 10.22146/jfi.24402

Abstract

Pengembangan sistem deteksi gas hembus pernapasan manusia yang memiliki kepekaan tinggi telah dilakukan dalam penelitian ini. Laser QCL (Quantum Cascade Laser) kontinyu dengan panjang gelombang 4610 nm sebagai sumber radiasi yang dikombinasikan dengan teknik spektroskopi off-axis ICOS (integrated cavity output spectroscopy) digunakan untuk medeteksi gas Karbon Monoksida (CO) bertekanan rendah. Sel sampel gas dengan panjang 15 cm yang dilengkapi dengan cermin high-finesse sekaligus berfungsi sebagai rongga resonator laser dengan lintasan optik efektif mencapai 400 m. Batas deteksi sebesar 1 ppbv dalam waktu akuisisi kurang dari 2 detik telah berhasil dicapai. Pendeteksian kasar pada gas hembus pernapasan manusia telah berhasil mendeteksi gas CO sebesar 0,7 ppmv.  
Kinerja Spektrometer Fotoakustik Laser CO2 untuk Deteksi Gas Etilen (C2H4), Aseton (C3H6O), Amonia (NH3) pada Gas Hembus Perokok Mahardika Yoga Darmawan; Mitrayana -; Ali Joko Wasono
Jurnal Fisika Indonesia Vol 19, No 57 (2015)
Publisher : Department of Physics Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6432.059 KB) | DOI: 10.22146/jfi.27213

Abstract

Kinerja spektrometer fotoakustik (SFA) telah dilakukan optimasi dan diaplikasikan. Karakterisasi SFA laser CO2 meliputi pemayaran garis laser CO2 dan garis serapan, penentuan kurva resonansi dan faktor kualitas, pengukuran noise sehingga diperoleh untuk faktor kualitas sebesar (14,5 + 0,6), (14,8 + 0,7), dan (14,2 + 0,6) dan batas deteksi terendah untuk gas etilen, aseton, amonia pada garis 10P14, 10P20, 10R14 yaitu (57,1 + 0,4) ppb, (63,8 + 2,4) ppb, dan (81,1 + 0,6) ppb. Selanjutnya SFA digunakan untuk mengukur konsentrasi gas etilen, aseton, amonia pada gas hembus perokok. Hasil pengukuran diolah menggunakan analisis multikomponen dan diperoleh konsentrasi rata-rata gas etilen, aseton, amonia pada gas hembus perokok tertinggi sebesar 1,303 ppm, 0,924 ppm, dan 1,753 ppm. Pada kondisi sebelum merokok diperoleh konsentrasi rata-rata gas etilen, aseton, amonia sebesar 0,873 ppm, 0,286 ppm, dan 1,137 ppm dan pada kondisi satu jam setelah merokok, konsentrasi gas hembus perokok diperoleh konsentrasi rata-rata gas etilen, aseton, amonia sebesar 0,901 ppm, 0,305 ppm, dan 1,176 ppm. Berdasarkan penelitian yang telah dilakukan ditemukan pola yang cukup identik yang ditunjukkan untuk ketiga jenis gas yaitu setelah 60 menit relawan merokok maka konsentrasi akhir gas etilen, aseton dan amonia akan sama dengan konsentrasi awalnya sebelum relawan merokok.kata kunci: Spektrometer Fotoakustik Laser CO2; perokok; analisis multikomponen
Penentuan Parameter Difusi Gas Buah Tomat dengan Spektrometer Fotoakustik Laser CO2 Menggunakan Pelacak Gas SF6 Moh Ali Joko Wasono
Jurnal Fisika Indonesia Vol 23, No 3 (2019)
Publisher : Department of Physics Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1516.022 KB) | DOI: 10.22146/jfi.v23i3.41404

Abstract

Telah dilakukan monitoring beberapa parameter difusi (waktu relaksasi, koefisien resistansi dan koefisien difusi) gas SF6 di dalam buah tomat (Lycopersicon esculentum) dengan menggunakan metode spektroskopi foto-akustik (SFA) laser CO2. Gas SF6 menyerap paling kuat pada garis 10P16 dan etilen pada garis laser 10P14 dengan frekuensi resonansi sebesar 1650±5 Hz. Batas deteksi terendah (BDT) yang dihasilkan oleh SFA untuk gas SF6 diperoleh 2,6±0,4ppt dan untuk etilen sebesar 0,32±0,03ppb. Monitoring difusi gas SF6 pada buah tomat dan pengukuran emisi etilen dalam kurun waktu 15 hari menghasilkan pola karakteristik waktu relaksasi, koefisien resistansi dan koefisen difusi terhadap emisi etilen. Dalam proses pematangan, waktu relaksasi dan koefisien resistansi buah tomat mengalami penurunan masing-masing dari 2,85±0,07 menit ke 0,86±0,01 menit dan dari (5,95±0,27)x102 detik cm-1 ke (1,53±0,06)x102 detik cm-1.Sedang  koefisien difusi mengalamai kenaikan dari (1,73±0,01)x10-2 cm2 detik-1 ke (6,70±0,03)x10-2 cm2 detik-1. Dari hasil tersebut dapat direkomendasikan kepada pengelola pengendalian pematangan buah tomat untuk mendapatkan optimasi parameter difusi sehingga diperoleh pematangan sesuai dengan kebutuhan pasar.
Penentuan Waktu Relaksasi Pertukaran Gas, Koefisien Difusi dan Resistensi Buah Tomat terhadap C2H4 Menggunakan Spektrometer Fotoakustik Laser CO2 dengan Pelacak Gas SF6 Moh Ali Joko Wasono; Y. A. Kholishoh
Jurnal Fisika Indonesia Vol 24, No 1 (2020)
Publisher : Department of Physics Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfi.v24i1.50559

Abstract

Telah dilakukan studi metode penentuan waktu relaksasi, koefisien resistansi dan koefisien difusi pertukaran gas SF6 di dalam buah tomat (Lycopersicon esculentum) dengan metode spektroskopi fotoakustik laser CO2 yang bersifat tidak mengganggu dan tidak merusak sampel buah. Gas SF6 menyerap paling kuat pada garis laser 10P16 dan gas C2H4 pada garis laser 10P14 dengan frekuensi resonansi sebesar (1650 ± 5) Hz. Batas deteksi terendah yang dihasilkan oleh spektrometer tsb untuk gas SF6 diperoleh (2; 6 ± 0; 4) ppt dan untuk gas C2H4 sebesar (0; 32 ± 0; 03) ppb. Sesudah diketemukan paramater difusi utk gas pelacak SF6, maka dapat ditentukan parameter difusi untuk hormon pematangan buah, gas C2H4. Sifat-sifat Metode pemantauan difusi gas SF6 pada buah tomat serta pengukuran emisi gas C2H4 dalam kurun waktu 15 hari menghasilkan pola karakteristik waktu relaksasi & koefisien resistansi serta koefisen difusi terhadap emisi etilen. Diperoleh hasil pola emisi C2H4 menunjukkan kurva sesuai dengan yang dimiliki buah klimakterik. Hasil pemantauan gas SF6 ditemukan kecenderungan waktu relaksasi dan koefisien resistensi buah terhadap gas SF6 menurun secara eksponensial dengan berjalannya waktu umur buah tomat. Adapun koefisien difusi mengalamai kenaikan secara eksponensial juga. Dari hasil perolehan transportasi gas didalam buah tomat menentukan metabolisme yang berkaitan langsung dengan penyimpanan buah Hal ini dapat direkomendasikan kepada pengelola pengendalian pematangan buah tomat untuk mendapatkan optimasi parameter difusi sehingga diperoleh pematangan sesuai dengan kebutuhan pasar.
Application of a Photoacoustic Tomography System: A Case Study on the Monitoring of Pig Tissue Decomposition Oey, Lusiana Sandra; Mitrayana, Mitrayana; Wasono, Moh. Ali Joko
Makara Journal of Science Vol. 26, No. 1
Publisher : UI Scholars Hub

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Abstract

Pig carcasses have been used in forensic research because they share several similarities to human cadaver, including decomposition. In several studies, the decomposition of pig’s cadaver for a certain time can be used as a model to determine the time of death of a human. This study was conducted to determine the relationship between the days of spoilage of pig tissue and the level of average sound intensity produced by the sample. Then, in this study, pig skeletal muscles were allowed to decay with a variation of 1–5 days. Afterward, these muscles were imaged using diode laser-based photoacoustic tomography. Results of the experiment show that the average acoustic intensity level from the first day until the fourth day has increased (78–92 a.u for young pig and 76–86 a.u for old pig) but decreased on the fifth day (88 a.u for young and 84 a.u for old pig). These results can improve forensic imaging because such results can be applied to determine the time of death of human by plotting the ratio of the average sound intensity level of the sample to the number of days of decomposition.