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Detection of White Spot Syndrome Virus (WSSV) of Shrimp (Litopenaeus vannamei) in UPT Pengembangan Budidaya Air Payau Bangil , Pasuruan District , East Java Rima Oktavia Kusuma; Muh. Sulaiman Dadiono; Baruna Kusuma; joni Johanda Putra
Journal of Aquaculture Development and Environment Vol 3, No 2 (2020): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v3i2.3426

Abstract

Vanname shrimp (Litopenaeus vanname) is still a favourite in shrimp culture production in Indonesia, since it has faster growth advantages. However, the White spot syndrome virus (WSSV) attack is still a big threat for farmers, because it can proliferates rapidly and cause mass mortality of 90-100%. The UPT Pengembangan Budidaya Air Payau in Bangil maintains vanname and controls virus infection by detecting through a molecular approach. Detection using the Nested PCR method with IQ 2000 kit with molecular weight marker 848 bp, 550 bp, 296 bp. The sample will be tested positive if the bands that appear are in the size 296 bp and / or 550 bp . The results of the detection in shrimp rearing ponds were negative (-) because only the band 848 bp that showed, it was mean the shrimp free from WSSV infection. Water quality data shows that the shrimp rearing environment is in optimal conditions.
The Effect of Work Experience, Job Satisfaction and Work Discipline On Working Achievement with Job Placement as Intervening Variables on PT. Ramayana Lestari Sentosa Tbk. Sidoarjo Dewi Andriani; Dita Listyana Anggraeni; Joni Johanda Putra; Monica Rosiana
Jurnal Akuntansi, Manajemen dan Ekonomi Vol 24 No 2 (2022): April - Juni 2022
Publisher : Faculty of Economics and Business, Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32424/1.jame.2022.24.2.6683

Abstract

This study aims to determine the effect of Experience, Satisfaction, and Work Discipline on Job Performance and the influence of Experience, Satisfaction, and Work Discipline on Job Performance with Placement as an Intervening Variable at PT Ramayana Lestari Sentosa Tbk. The approach used in this research is quantitative. The population in this study was 250 employees. This research used non probability sampling especially purposive sampling. The sample in this study was 96 respondents—analyzed using SmartPLS 3.0 software. The results show that experience, satisfaction, and work discipline have a significant effect on work performance, experience has a significant effect on job performance, satisfaction has a significant effect on job performance, and discipline has a significant effect on job performance. In addition, the results show that experience, satisfaction, and work discipline significantly affect job performance through job placement as an intervening variable.
EKOSISTEM BISNIS PEMBESARAN IKAN BANDENG (CHANOS CHANOS) DI DESA GRINTING, KECAMATAN BULAKAMBA, KABUPATEN BREBES Taufik Wahyu; Nahl Firdaus Alatas; Joni Johanda Putra; Taufik Budhi Pramono
Jurnal Pengabdian Perikanan Indonesia Vol 2 No 3 (2022): Jurnal Pengabdian Perikanan Indonesia
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppi.v2i3.1718

Abstract

Milkfish (Chanos chanos) is a leading fish commodity in Brebes Regency. The sustainable development of milkfish requires a supportive business ecosystem. The purpose of this study was to determine the business ecosystem of milkfish enlargement business in Grinting Village, Bulakamba District, Brebes Regency. Research data collection was carried out in March-April 2022 in Grinting Village, Bulakamba District, Brebes Regency. This research method uses an active survey with purposive sampling. Business ecosystem data is obtained directly through interviews with groups of fish farmers using a questionnaire. The results of the business process analysis show that the supply of milkfish nener in Grinting village comes from Bali. There is 1 member of the group who specializes in nursing efforts to ensure the supply of nener for rearing. The cultivation process generally applies the traditional system which is carried out for 4-6 months. The production output is milkfish consumption with the size of 6-7 fish/Kg and 1-2 fish/Kg. The market or customer for milkfish consumption is still within the scope of the Central Java region. Milkfish cultivators have built a business ecosystem through the application of SIPOC in a close system in a sustainable aquaculture business.