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All Journal Jurnal Perikanan dan Kelautan Jurnal Sains dan Inovasi Perikanan/Journal of Fishery and Innovation (JSIPi) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Perikanan Universitas Gadjah Mada Fish Scientiae : Jurnal Ilmu-Ilmu Perikanan dan Kelautan
Olga Olga
Jurusan Budidaya Perairan, Fakultas Perikanan Universitas Lambung Mangkurat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics

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Analisis Fitokimia dan Aktivitas Antibakteri Beberapa Jenis Tanaman terhadap Bakteri Patogen pada Budidaya Ikan Salosso, Yuliana; Olga, Olga; Aisiah, Siti; Ressie, Jeny Dorotea; Foes, Yenni Welhelmina; Pasaribu, Wesley
JSIPi (Jurnal Sains dan Inovasi Perikanan) (Journal of Fishery Science and Innovation) Vol 5, No 2 (2021): JURNAL SAINS dan INOVASI PERIKANAN
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jsipi.v5i2.17498

Abstract

Penelitian ini bertujuan untuk mengetahui kandungan fitokimia serta aktivitas antibakteri sepuluh jenis tanaman  terhadap bakteri Vibrio alginolyticus dan Aeromonas hydropilla. Dipilih  10 jenis tanaman yang sering digunakan sebagai obat dan banyak ditemukan di kota Kupang seperti tanaman Phyllanthus acidus, Euphorbia hirta, Persea gratissima, Euphorbia thymifolia,  Morus australis, Justica gendarussa, Paederia scandeus, Annona squamosa,  Sesbania sesban,  dan Jatropha gossypifolia. Bagian tanaman yang digunakan adalah daun, kecuali E. hirta dan E. thymifolia digunakan seluruh bagian tanaman.  Kesepuluh jenis tanaman tersebut dibuat bubuk,  kemudian diekstraksi dengan cara dimaserasi secara bertingkat mulai dari pelarut n-heksan, etil asetat dan metanol, selain itu, juga digunakan ekstrak air  tanaman tersebut. Semua jenis ekstrak tanaman diuji antibakteri dengan menggunakan metode cakram. Jenis ekstrak yang aktif selanjutnya diuji antibakteri pada konsentrasi 10%, 1% dan 0,1%.  Hasil penelitian menunjukkan bahwa jenis  tanaman  yang berpotensi sebagai antibakteri pada budidaya ikan laut adalah  esktrak air dan metanol E. hirta dan E. thymifolia. Sedangkan jenis tanaman yang berpotensi sebagai antibakteri pada budidaya ikan air tawar adalah ekstrak air  E. hirta dan E. thymifolia daun J. gendarussa. 
Aeromonas hydrophila Bivalen Vaccines to Improve Body Resistance catfish (Pangasius hypophthalmus) Against Attacks Motile Aeromonas Septicemia Dwi Mailani; Fatmawati Fatmawati; Noor Arida Fauzana; Olga Olga
Jurnal Perikanan dan Kelautan Vol 10, No 1 (2020)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jpk.v10i1.8286

Abstract

Motile aeromonas septicemia/MAS diseases due to Aeromonas hydrophila infection is an obstacle in cultivation of catfish (Pangasius hypophthalmus). MAS control can be done by administering vaccines. The purpose of this research is to get the right and effective dose of bivalent vaccine for controlling MAS at catfish. Both isolates will be used as vaccines are local strains of A. hydrophila CASO.01.G and SBMI.2. Both of them were isolated from disease catfish in the aquaculture ponds in Cindai Alus and Sungai Batang villages, Banjar Regency, South Kalimantan. Vaccines are made by heating (heat killed) at 100oC for 60 minutes. A total of  13 test catfish measuring 9-13 cm were included in each aquarium. The design used was a completely randomized design with 5 treatments and 3 replications, namely bivalent vaccine with a dose of 109 cells/ml, 108 cells/ml, 107 cells/ml, 106 cells/ml, and control (PBS solution pH 7.0). The highest antibody titers in treatments vaccinated fish at a dose of 107 cells / ml (1,706.67). The effectiveness of the vaccine can be seen by the high RPS value of the treatment dose 109 cells/ml (97.22%); 108 cells/ml (100%); 107 cells/ml (97.22%); and 106 cells/ml (100%). The control MTD value was 1 day with 97.43% mortality, while in the vaccine treatment with dose of 107 cells/ml, there was a MTD repeat value for 1 day, but only 1 fish died. Water quality of this research are supportive and not a cause of fish death during the challenge test
Protein Aeromonas Hydrophila Sebagai Vaksin untuk Pengendalian Mas (Motile Aeromonas Septicemia) pada Jambal Siam (Pangasius hypophthalamus) Olga Olga; Ririen Kartika Rini; Junius Akbar; Alim Isnansetyo; Langkah Sembiring
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.59

Abstract

Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to evaluate the efficacy of the protein to control Motile Aeromonas Septicemia (MAS) in catfish (Pangasius hypophthalamus). A. hydrophila was isolated from the kidney of diseased catfish. The isolate was cultured, sonicated and sentrifuged into cell-free extract and debris. The protein in the cell-free extract was precipitated with ammonium sulphate, dialized, then fractionated by gel filtration column chromatography on Sephadex G150.The concentration of protein were estimated by spectrophotometer at 280 nm. Fractions obtained from the gel filtration with high concentration of protein were analized by sodium dodocyl sulphate-polyachrylamide gel electrophoresis (SDS-PAGE). The protein bands obtained from SDS-PAGE with molecular weight more than 10 kDa were tested for immunogenicity to mice. Futhermore, the most immunogenic protein was used for fish vaccination. The vaccinated and unvaccinated fishes were chalenged with A. hydrophila.The results indicated that vaccination with the protein significantly increased (P<0.01) the antibody titer either in mice or catfish. Relative Percent Survival of catfish vaccinated with the protein at 5; 7.5; and 10 µg/fish were 61.54%, 80.77% and 76.92%, respectively. The optimum dose of vaccine was 8.33 µg/fish with maximum RPS of 82.05%.
EFIKASI RUTE VAKSIN Aeromonas hydrophila ASB-01 PADA IKAN GABUS (Ophiocephalus striatus) Olga Olga; Fatmawati Fatmawati
Fish Scientiae Vol 3 No 2 (2013): Issue December-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.8 KB)

Abstract

This study aims to determine the vaccine A.hydrophila ASB-01 is effective forthe control of MAS on snakehead fish. Effectiveness of vaccination was evaluated through the antibody titer, survival, RPS (relative percent survival) and RWK (meantime of death). The study consisted of 5 treatments (immersion vaccination (R), oral(O), intramuscular injection (IM), intraperitoneal injection (IP) and control (PBS pH7.0) with 3 replications. Vaccination doses were 107 cells / ml. Booster vaccination after 14 days later. Dose is equal to the initial vaccination. Furthermore, the next day14 fish were challenged with A.hydrophila ASB-01. To obtain data on antibody titerblood draw done at the time before being vaccinated, shortly before the booster vaccination and 14 days after the booster vaccination. Challenged fish were observedfor 14 days to obtain data on survival, RPS and RWK. The result showed that allthese vaccines may increase the antibody titer, but the highest antibody titers obtained from fish vaccinated injection. Survival rates were vaccinated IM (84.47%), IP(82.20%), R (42.27%), O (42.20%) and controls (13.13%). RPS to IM (82.08%), IP(79.46%), R (33.38%), O (33.31%), while RWK through the IP (3.63 days), IM(79.46 3.57 days), R (2.46 days), O (1.85 days) and controls (1.03 days). Effective vaccination route is through injection.
VIRULENSI BAKTERI Edwardsiella ictaluri PENYEBAB PENYAKIT ENTERIC SEPTICEMIA OF CATFISH (ESC) PADA IKAN PATIN (Pangasius pangasius) Nurul Huda Noor Azmi; Fatmawati Fatmawati; Olga Olga
Fish Scientiae Vol 11 No 1 (2021): Issue June-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.998 KB) | DOI: 10.20527/fishscientiae.v11i1.164

Abstract

Penyakit infeksius yang menyerang ikan patin, salah satunya disebabkan bakteri Edwardsiella ictaluri. Bakteri ini menyebabkan penyakit sistemik enteric septicemia of catfish (ESC). Penelitian bertujuan untuk mengetahui gejala klinis dan rerata waktu kematian akibat serangan bakteri E. ictalury pada ikan patin. Bakteri diinokulasi ke media BHIA (Brain Heart Infusion Agar, merek Conda Pronadisa) dan BHIB (Brain Heart Infusion Broth, merek Merck) diinkubasi pada suhu 24 oC selama 24 - 48 jam. Suspensi bakteri dengan kepadatan 2,7 x 101 – 1010 cfu/mL diinfeksikan pada 300 ekor ikan patin berukuran 11-12 cm dan diamati selama 7 hari. Penelitian menggunakan metode eksperimental dan Completely Randomized Design (CRD). Analisis lebih lanjut dengan Duncan multiple range test (DMRT). Hasil Penelitian menunjukkan gejala klinis yang muncul pada ikan patin antara lain, ikan berenang secara vertikal, warna tubuh pucat, nekrosis, petechiae, liver pucat dan membesar, ginjal berwarna gelap dan kaku, asites insang pucat. Nilai rerata waktu kematian (RWK) ikan patin berkisar antara 1,16 – 2,26 hari. Infectious diseases that attack catfish, one of which is caused by the bacterium Edwardsiella ictaluri. This bacterium causes systemic enteric septicemia of catfish (ESC). The aim of the study was to determine the clinical symptoms and the mean time of death due to the attack of E. ictaluri bacteria on catfish. Bacteria were inoculated into BHIA media (Brain Heart Infusion Agar, Conda Pronadisa brand) and BHIB (Brain Heart Infusion Broth, Merck brand) were incubated at 24 oC for 24-48 hours. Bacterial suspension with a density of 2.7 x 101 – 1010 cfu/mL was infected with 300 catfish measuring 11-12 cm and observed for 7 days. The study used experimental methods and completely randomized design (CRD). Further analysis with Duncan multiple range test (DMRT). The results showed clinical symptoms that appeared in catfish, among others, fish swimming vertically, pale body color, necrosis, petechiae, pale and enlarged liver, dark and stiff kidneys, ascites, pale gills. The mean time of death (MTD) of catfish ranged from 1.16 to 2.26 days.
SUPLEMENTASI Artemia sp DAN Daphnia sp UNTUK PERTUMBUHAN DAN SINTASAN LARVA IKAN PAPUYU (Anabas testudineus) Muhammad Alfian; Herliwati Herliwati; Olga Olga
Fish Scientiae Vol 12 No 1 (2022): Issue June-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/fishscientiae.v12i1.187

Abstract

Penelitian ini bertujuan untuk mengkaji pertumbuhan dan sintasan larva ikan papuyu (Anabas testudineus Bloch) dengan pemberian Artemia sp dan Daphnia sp bersuplemen Viterna dan mengetahui pakan alami bersuplemen terbaik untuk dijadikan pakan larva papuyu. Larva ikan papuyu dipelihara selama 34 hari. Rancangan yang digunakan pada penelitian ini adalah rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan yang digunakan antara lain A (Daphnia sp non suplemen), B (Artemia sp non suplemen), C (Daphnia sp bersuplemen), D (Artemia sp bersuplemen). Hasil penelitian ini menunjukkan bahwa pemberian Artemia sp dan Daphnia sp bersuplemen berpengaruh nyata terhadap pertumbuhan panjang dan berat relatif, dan sintasan larva papuyu. Perlakuan terbaik untuk panjang relatif, yakni C (266,67%) yang diikuti D (254,94%), berat relatif pada perlakuan D (187,78%) dan sintasan pada perlakuan D (77%) dan C (70%). Sedangkan kualitas air media pemeliharaan, yakni suhu 25 -27,4 oC, DO 2,01-3,10 mg/L, pH 6,3 – 7,9, dan amoniak (NH3) 0,15 – 0,26 mg/L masih dalam kisaran yang baik untuk kehidupan papuyu. This study aimed to study the growth and survival of climbing perch (Anabas testudineus Bloch) larvae with Artemia sp and Daphnia sp supplemented with Viterna and to find out the best natural food supplemented to be used as feed for climbing perch larvae. Climbing perch fish larvae were reared for 34 days. The design used in this study was a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments used included A (Daphnia sp non-supplemented), B (Artemia sp non-supplemented), C (Daphnia sp supplemented), D (Artemia sp supplemented). The results of this study showed that the supplementation of Artemia sp and Daphnia sp had a significant effect on the growth of relative length and weight, and the survival of climbing perch larvae. The best treatment for relative length, namely C (266.67%) followed by D (254.94%), relative weight in treatment D (187.78%) and survival in treatment D (77%) and C (70%) . While the water quality of the maintenance media, namely the temperature 25 -27.4 oC, DO 2.01-3.10 mg/L, pH 6.3 - 7.9, and ammonia (NH3) 0.15 - 0.26 mg/L is still in a good range for climbing perch's life.
IMUNOGENISITAS ANTIGEN HEAT KILLED Aeromonas hydrophila STRAIN LOKAL DESA SUNGAI BATANG DAN MANDIANGIN TERHADAP IKAN LELE DUMBO (Clarias gariepinus) Alna Chairunisa; Olga Olga; Siti Aisiah
Fish Scientiae Vol 13 No 1 (2023): Issue June-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/fishscientiae.v13i1.203

Abstract

Penelitian ini bertujuan untuk menyeleksi dan mendapatkan antigen bakteri A.hydrophila strain lokal desa Sungai Batang dan Mandiangin Kalimantan Selatan yang bersifat imunogenik terhadap ikan lele dumbo. Antigen diinaktif menggunakan metode pemanasan (heat killed). Rancangan penelitian ini menggunakan Rancangan acak lengkap (RAL) dengan 6 perlakuan 3 ulangan yang meliputi perlakuan vaksinasi dengan antigen Aeromonas hydrophila strain P5.1 (A), strain AM01 (B), strain P1.1 (C), strain P3.1 (D), strain P4.1 (E) dan kontrol diinjeksi dengan PBS pH 7,0 (K). Antigen diinjeksi secara intramuscular sebanyak 0.1 ml (kepadatan bakteri 109 sel/ml) per ekor ikan uji (A,B,C,D,E). Hasil uji imunogenisitas antigen strain lokal P5.1 dan P1.1 bersifat lebih imunogenik terhadap lele dumbo dibandingkan dengan antigen lainnya berdasarkan kemampuannya meningkatkan antibodi pasca vaksinasi. Uji reaksi silang antigen A. hydrophila strain lokal AM01, P1.1 dan P5.1 mampu bereaksi silang dengan antigen lainnya. Antigen P1.1 mampu mengenali antigen lebih banyak dibandingkan P5.1 dan AM01, sehingga dapat direkomendasikan menjadi kandidat vaksin. This study aims to select and obtain antigens of the local strain A. hydrophila bacteria in Sungai Batang and Mandiangin villages, South Kalimantan, which are immunogenic to African catfish. Antigen is inactivated using the heating method (heat killed). The design of this study used a completely randomized design (CRD) with 6 treatments and 3 replications which included vaccination with the antigen Aeromonas hydrophila strain P5.1 (A), strain AM01 (B), strain P1.1 (C), strain P3.1 ( D), strain P4.1 (E) and control were injected with PBS pH 7.0 (K). Antigen was injected intramuscularly as much as 0.1 ml (bacterial density 109 cells/ml) per test fish (A, B, C, D, E). The results of the antigen immunogenicity test of local strains P5.1 and P1.1 were more immunogenic to African catfish compared to other antigens based on their ability to increase post-vaccination antibodies. Antigen cross-reaction test A. hydrophila local strains AM01, P1.1 and P5.1 are capable of cross-reacting with other antigens. P1.1 antigen is able to recognize more antigens than P5.1 and AM01, so it can be recommended as a vaccine candidate.
Hematological and Histological Analysis of Tilapia (Oreochromis Niloticus) Cultured in Floating Net Cages After Disease Outbreak Ririen Kartika Rini; Siti Aisiah; Olga; Lutfia Nafisah
Jurnal Penelitian Pendidikan IPA Vol 10 No 4 (2024): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i4.5057

Abstract

Recently, Tilapia fish cultivated in floating net cages in Karang Intan District experienced a sudden death from June 4 to June 5. At some point, mortality reached 50% of the population in floating net cages. The purpose of this study was to analyze the hemtology and histology of tilapia (Oreochromis niloticus) after exposure to disease outbreaks that were cultivated in floating net cages. The research location was carried out at the Laboratory of Fish Pests and Diseases, Faculty of Fisheries and Maritime Affairs, University of Lambung Mangkurat. The animal samples used were tilapia showing clinical symptoms of sick fish obtained from floating net cages in Mali-Mali Village, Karang Intan District, which experienced quite high fish mortality cases and 3 fish were taken. Based on the results of hemtological analysis of tilapia (Oreochromis niloticus) after being exposed to disease outbreaks which were cultivated in floating net cages, the hemoglobin levels of tilapia obtained in this study ranged from 6.9-7.8 g/dl, the average hematocrit of tilapia was obtained in this study has a range between 37.2 - 58.9%, the average tilapia leukocrit obtained in this study has a range between 2.89 - 6.61% and blood plasma is a liquid component of blood consisting of 99% water and 8-9% protein.
Co-Authors Alim Isnansetyo Alna Chairunisa Dwi Mailani Fatmawati, Fatmawati Fatmawaty Fatmawaty, Fatmawaty Foes, Yenni Welhelmina Herliwati Herliwati Junius Akbar Langkah Sembiring Lutfia Nafisah Muhammad Alfian Noor Arida Fauzana Nurul Huda Noor Azmi Pasaribu, Wesley Ressie, Jeny Dorotea Ririen Kartika Rini Ririen Kartika Rini Siti Aisiah Siti Aisiah Siti Aisiah Yuliana Salosso