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All Journal Jurnal Perikanan Universitas Gadjah Mada Tropical Wetland Journal
Ririen Kartika Rini
Faculty Of Fisheries And Marine Science, Lambung Mangkurat University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Immunology & microbiology Medicine & Pharmacology Other

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The Breeding of Climbing Perch (Anabas Testudineus) With Meristic Phylogenetic Hybridization Technique Sampled From Three Types of Swamp Ecosystems Slamat Slamat; Pahmi Ansyari; Ahmadi Ahmadi; Ririen Kartika
TROPICAL WETLAND JOURNAL Vol 5 No 2 (2019): Tropical Wetland Journal
Publisher : Postgraduate Program - Lambung Mangkurat University (ULM Press Academic)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/twj.v5i2.72

Abstract

This study provides a valuable information on the patterns of hybridization in producing Climbingperch’s fry which having a superior character to beneficially supports fish farming. The research wascarried out at Pokdakan Rawa Sejahtera Amuntai, South Kalimantan Province, Indonesia from June toDecember 2018. The hybridization procedures were applied to the selected broodstocks (total 135),which taken from three different types of habitats namely rain reservoirs, monotonous and tidalswamps. A complete random design was used as a research method with three treatments and ninerepetitions. A comprehensive investigation was done to provide the best performance among thetreatments. The results showed that the hybridized fish from monotonous swamp x tidal swamp wasthe most superior across the trials in term of fecundity (18,500 eggs), GSI (18.1%), hatching rate(89.5%), relative length growth (1,375%), relative weight growth (1,850%) and the percentage of birthfemale (84 %) with the lowest mortality rate (29%). For aquaculture practices, the use of the fryunisexual female would increase the fish growth 270% faster than the fry male.
Protein Aeromonas Hydrophila Sebagai Vaksin untuk Pengendalian Mas (Motile Aeromonas Septicemia) pada Jambal Siam (Pangasius hypophthalamus) Olga Olga; Ririen Kartika Rini; Junius Akbar; Alim Isnansetyo; Langkah Sembiring
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.59

Abstract

Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to evaluate the efficacy of the protein to control Motile Aeromonas Septicemia (MAS) in catfish (Pangasius hypophthalamus). A. hydrophila was isolated from the kidney of diseased catfish. The isolate was cultured, sonicated and sentrifuged into cell-free extract and debris. The protein in the cell-free extract was precipitated with ammonium sulphate, dialized, then fractionated by gel filtration column chromatography on Sephadex G150.The concentration of protein were estimated by spectrophotometer at 280 nm. Fractions obtained from the gel filtration with high concentration of protein were analized by sodium dodocyl sulphate-polyachrylamide gel electrophoresis (SDS-PAGE). The protein bands obtained from SDS-PAGE with molecular weight more than 10 kDa were tested for immunogenicity to mice. Futhermore, the most immunogenic protein was used for fish vaccination. The vaccinated and unvaccinated fishes were chalenged with A. hydrophila.The results indicated that vaccination with the protein significantly increased (P<0.01) the antibody titer either in mice or catfish. Relative Percent Survival of catfish vaccinated with the protein at 5; 7.5; and 10 µg/fish were 61.54%, 80.77% and 76.92%, respectively. The optimum dose of vaccine was 8.33 µg/fish with maximum RPS of 82.05%.
Co-Authors Alim Isnansetyo Junius Akbar Langkah Sembiring Olga Olga Pahmi Ansyari Slamat Slamat