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Journal : Teknosains: Media Informasi Sains dan Teknologi

IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM L-ASPARAGINASE DARI ALGA COKLAT SARGASSUM sp Masri, Mashuri
Teknosains Vol 8, No 2 (2014): JULI
Publisher : Fakultas Sains dan Teknologi UIN ALauddin

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Molecular Identification of bacterial L-asparaginase activity from Sargassum sp were isolated from Barrang Lompo Island South Sulawesi in 2013. Among them three bacterial strains exhibiting glutaminase free L-asparaginase activity were identified as Pseudomonas putida, Enterobacter asburine dan Marinobacter arcticus on the basis of 16S rRNA gene sequencing. The Highest Max Score 1358 and Query Cover 98 % were Pseudomonas putida furthermore identified as bacterial L-asparaginase activity from Sargassum sp. Pseudomonas putida has Identities 756/765 (99%) with 778 bp Query length, Query cover 98% and Gaps 5/765 (0%).Key words: L-Asparaginase, Sargassum sp..
DETEKSI KOI HARPES VIRUS (KHV) PADA IKAN MAS KOI (Cyprinus carpio L) DENGAN MENGGUNAKAN METODE APLIKASI Polymerase Chain Reaction(PCR) Mashuri Masri; Arfan Arifuddin
Teknosains Vol 7 No 2 (2013): JULI
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v7i2.85

Abstract

This thesis discusses the detection of koi harpes virus (KHV) in koi carp (Cyprinus carpio L) Application Method Using the Polymerase Chain Reaction (PCR). The purpose of this study is to determine the level of virus attacks causing harpesviridae group KHV Koi carp (Cyprinus carpio L.) using PCR applications in the Central Fish Karantian Hasanuddin Makassar. The research method used is descriptive with pictures PCR of Polaroid photographs. The results obtained with the results of attacks lightly infected 290 bp, the attack was infected with the results of 290 bp and 440 bp, with the result of severeinfection attack 290 bp 440 bp and 630 bp.
IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM L-ASPARAGINASE DARI ALGA COKLAT SARGASSUM sp Mashuri Masri
Teknosains Vol 8 No 2 (2014): JULI
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v8i2.1913

Abstract

Molecular Identification of bacterial L-asparaginase activity from Sargassum sp were isolated from Barrang Lompo Island South Sulawesi in 2013. Among them three bacterial strains exhibiting glutaminase free L-asparaginase activity were identified as Pseudomonas putida, Enterobacter asburine dan Marinobacter arcticus on the basis of 16S rRNA gene sequencing. The Highest Max Score 1358 and Query Cover 98 % were Pseudomonas putida furthermore identified as bacterial L-asparaginase activity from Sargassum sp. Pseudomonas putida has Identities 756/765 (99%) with 778 bp Query length, Query cover 98% and Gaps 5/765 (0%).