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Expression of Mycobacterium tuberculosis Protein Tyrosine Phosphatase B in Escherichia coli and Its Recovery from Inclusion Body Savalas, Lalu Rudyat Telly; Sedijani, Prapti; Hadisaputra, Saprizal; Ardhuha, Jannatin; Lestari, Chomsa Asih; Wahidah, Etty Nurul
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12384

Abstract

The present study aims at expressing and partially purifying PtpB in active form. To achieve this, Mtb PtpB gene has been cloned into pET30a vector and overexpressed in Escherichia coli BL 21(DE3) under IPTG induction in the form of an inclusion body. Following resolubilization by urea and dialysis, the resulted PtpB has been shown to be active against para-Nitrophenyl phosphate. It is concluded that the resulted PtpB has had been recovered from inclusion body to give the active form of the enzyme, and thus the success in overexpressing PtpB provides the required material to investigate the biochemical properties of the pathogen virulence factor further.
Pelatihan Penyusunan Media Permainan Edukatif Pada Guru-Guru MIN 3 Cakranegara Verawati, Ni Nyoman Sri Putu; Rokhmat, Joni; Kesipudin, Muhammad; Gunada, I Wayan; Ardhuha, Jannatin
Lumbung Inovasi: Jurnal Pengabdian kepada Masyarakat Vol 3, No 1: May 2018
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/linov.v3i1.407

Abstract

Tujuan dari kegiatan pengabdian kepada masyarakat yang dilakukan oleh tim pengabdian yaitu membimbing guru-guru dalam membuat media permainan edukatif yang meliputi program aplikasi findthatword dan eclipse pada Guru-Guru MIN 3 Cakranegara. Metode yang digunakan adalah metode ceramah, pembimbingan penyusunan media permainan edukatif, diskusi dan Tanya jawab. Hasil pengabdian para guru dapat mendesain sendiri media permainan edukatif yang meliputi media permainan acak kata dan TTS sesuai dengan materi pelajaran yang  diajarkan masing-masing, para guru sangat antusias dan semangat mengikuti kegiatan pengabdian ini karena bagi mereka kegiatan ini sangat bermanfaat, membantu para guru dalam mempersiapkan proses pembelajaran dan merupakan salah satu alternative media permaianan yang efektif sehingga proses  pembelajaran berjalan optimal dan menyenangkan bagi peserta didik.Training of Educative Game Media Development in MIN 3 Teachers CakranegaraAbstract: The purpose of the community service activities carried out by the dedication team is to guide teachers in making educational media games that include findthatword and eclipse application programs for MIN 3 Cakranegara Teachers. The method used is the lecture method, guiding the preparation of educational media games, discussions and question and answer. The results of the dedication of the teachers can design their own educational media games which include random word and TTS game media in accordance with the subject matter taught respectively, the teachers are very enthusiastic and enthusiastic in participating in this service because for them this activity is very useful, helping the teachers in preparing the learning process and is an alternative game media that is effective so that the learning process is optimal and enjoyable for students.
Expression of Mycobacterium tuberculosis Protein Tyrosine Phosphatase B in Escherichia coli and Its Recovery from Inclusion Body Savalas, Lalu Rudyat Telly; Sedijani, Prapti; Hadisaputra, Saprizal; Ardhuha, Jannatin; Lestari, Chomsa Asih; Wahidah, Etty Nurul
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12384

Abstract

The present study aims at expressing and partially purifying PtpB in active form. To achieve this, Mtb PtpB gene has been cloned into pET30a vector and overexpressed in Escherichia coli BL 21(DE3) under IPTG induction in the form of an inclusion body. Following resolubilization by urea and dialysis, the resulted PtpB has been shown to be active against para-Nitrophenyl phosphate.  It is concluded that the resulted PtpB has had been recovered from inclusion body to give the active form of the enzyme, and thus the success in overexpressing PtpB provides the required material to investigate the biochemical properties of the pathogen virulence factor further.