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All Journal BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Makara Journal of Science
Nandang Suharna, Nandang
Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Cibinong, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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VARIASI GENETIK MONASCUS PURPUREUS YANG DIISOLASI DARI BERAS MERAH CINA Suharna, Nandang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3470

Abstract

ABSTRACTGenetic Variation of Monascus purpureus Isolated from Chinese Red Rice. An attempt was carried out to detect genetic variation among six Monascus purpureus isolates by RAPD finger printing. The isolates studied were isolated from Chinese red rice (ang-kak) collected from Indonesian markets. Seven types of oligonucleotide 12-mer primers were used in this study. These primers were different from G+C ratio (42%, 50%, 68% and 75%) so as to know the best ratio for DNA amplication. Products of DNA amplication by seven primers were observed such as 158 banding patterns, comprise of 23 common fragments and 135 polimorfic fragments. The result showed that the six isolates were divided into two groups based on DNA banding patterns. The same grouping was also shown by phylogenetic analysis. This analysis indicated two cluster which were different from its genetic distance (49%) and two pairs of isolates were identical to each other. So far, this RAPD analysis proved that there was high genetic variation within the six isolat of M. purpureus. It was assumed that there was a tendency of species separation within those isolates. It is suggested that sequencing analysis on 18S rDNA should be carried out to ensure status of M.purpureus species used in ang-kak production.Key words: Monascus purpureus, variation, genetic, Chinese red-rice, RAPD
Characterization of Cellulase Enzyme Produced by Two Selected Strains of Streptomyces Macrosporeus Isolated from Soil in Indonesia Soeka, Yati; Suharna, Nandang; Triana, Evi; Yulinery, Titin
Makara Journal of Science
Publisher : UI Scholars Hub

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Abstract

This study was conducted to characterize the cellulase enzymes produced by Streptomyces macrosporeus BB 32 and S.microspores KRC 21. D, which were isolated from Tanjung Pendam, Belitung Island, and from Cibodas Botanical Gar-den, Indonesia, respectively. The optimal activity of the enzymes was analyzed using parameters such as incubationtime, pH, temperature, carboxymethylcellulose (CMC) concentration. The effect of the addition of some metal ions asactivators or inhibitors was also analyzed spectrophotometrically at λ 540 nm. Results demonstrated that the activity of the cellulase enzymes of S. macrosporeus BB 32 and S. macrosporeus KRC 21 D reached the optimum level after 2 and5 days of incubation and at pH values of 8.0 and 6.0, temperatures of 35 °C and 50 °C, and CMC concentrations of 1.75% and 2%, respectively. S. macrosporeus BB 32 cellulase was activated by the cations CuCl2, MgCl2, and ZnCl2but inhibited by NaCl and CoCl2, reducing its activity. The cellulase of S. macrosporeusKRC 21.D was activated bythe cation NaCl and by the divalent cations CoCl2, CuCl2, MgCl2, and ZnCl2.S. macrosporeus BB 32 was deposited at the Indonesian Culture Collection with the collection number InaCC A144.
Co-Authors Desnora, Beni Desnora, Beni Evi Triana HEDDY JULISTIONO Rita Dwi Rahayu Soeka, Yati Yulinery, Titin