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All Journal Jurnal Pangan dan Agroindustri LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat ALKIMIA : Jurnal Ilmu Kimia dan Terapan Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat
Maylina Ilhami Khurniyati
INSTITUT TEKNOLOGI DAN SAINS NAHDLATUL ULAMA PASURUAN
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Social Sciences Other

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Journal : ALKIMIA : Jurnal Ilmu Kimia dan Terapan

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Skrining Bakteri Lipolitik Hasil Isolasi dari Tanah Terkontaminasi Minyak Khurniyati, Maylina Ilhami; Sumarsih, Sri; Chasanah, Uswatun
ALKIMIA Vol 6 No 1 (2022): ALKIMIA
Publisher : SCIENCE AND TECHNOLOGY FACULTY OF UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.546 KB) | DOI: 10.19109/alkimia.v6i1.12297

Abstract

Lipase (triasilglycerol acylhydrolase, E.C, 3.1.1.3) is an enzyme can hydrolyze long chains of triglycerides[1]. Lipase is a water-soluble enzyme and naturally lipase catalyze the hydrolysis of ester bonds in water insoluble lipid substrate and long-chain acylglycerol synthesis, diacylglycerol, monoacylglycerol, and glycerol[2]. Hydrolase enzyme lipase can be used potentialy produced fatty acid which precursors in the chemical industry[3]. The research aims to study the lipolytic activity of bacteria isolated from soil contaminated with oil. Screening of lipolytic bacteria was performed in Rhodamin-B agar plate containing olive oil. The lipolytic activity was determined by spectrofotometry method toward p-nitrofenil palmitat as a substrate. The result this research showed that there are 15 of 150 isolates bacteria from soil contaminated with oil was confirmed by observing the zone of hydrolysis formed around the growth of colony on Rhodamin B agar plate containing olive oil. One of the lipolytic bacteria identified as higher activity was produced lipase with activity of 7,41 U/ml
Co-Authors Abd Rohim Abd. Rohim Anis Nurhayati Anis Nurhayati Pinctada Putri Pamungkas Pinctada Putri Pamungkas Sri Sumarsih Teti Estiasih Uswatun Chasanah Yunita Khilyatun Nisak