One of the reproductive health problems in adolescent girls is vaginal discharge. This research is correlational analytic research with cross sectional approach. The sample was selected using purposive sampling with a sample of 40 respondents. Collecting research data using a questionnaire. Data analysis.was carried out in several stages, namely univariate analysis and also bivariate analysis using the Chi-Square test. Based on the results of the analysis showed that there was a significant relationship between attitudes (P-value 0.027), vulvar hygiene (P-value 0.001) and eating patterns (P-value 0.005) on the incidence of vaginal discharge. There is a relationship between attitudes, vulvar hygiene and eating patterns with the incidence of vaginal discharge in the midwifery study program students of UKB Palembang in 2022.