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All Journal JURNAL KAJIAN VETERINER
Maxs U. E. Sanam
Laboratory of Animal Diseases and Veterinary Public Health, Veterinary Faculty, Universitas Nusa cendana, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health Veterinary

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POTENTIAL ANTIMICROBIAL IN WHEY AND LACTIC ACID BACTERIA IN SUMBA MARE'S MILK Maxs U. E. Sanam
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.7868

Abstract

Sumba mare’s milk is rich in whey protein, fat, and lactic acid bacteria known to boost antimicrobial activities required in the prevention of diarrhea and inflammation. This study is, therefore, aimed at determining the antibacterial properties of whey and lactic acid bacteria as therapeutic compounds against pathogenic Staphylococcus aureus, SalmonellaTyphimurium, and Salmonella Enteritidis, respectively. The investigations were conducted at the Veterinary Public Health Laboratory of the Faculty of Veterinary Medicine, Nusa Cendana University. Whey antimicrobial test was performed using Salmonella Typhimurium ATCC® 19585 ™ and Staphylococcus aureus, while for lactic acid bacteria, SalmonellaEnteritidis ATCC® 13076 ™ obtained from the Veterinary Public Health Section, Faculty of Veterinary Medicine, Bogor Agricultural University, was applied. However, mare colostrum was acquired from East Sumba, East Indonesia. The results showed the average penicillin inhibitory zones of whey protein were estimated at 20 mm and 17 mm against Staphylococcus aureus. This also revealed the average diameter of the penicillin inhibitory zone and whey protein were equally evaluated at 2.1 mm, although the latter demonstrated sufficient antimicrobial activity against SalmonellaTyphimurium. Furthermore, the lactic acid bacteria inhibitory zone in filtrate and non-filtrates are strongly characterized using the agar method against Salmonella Enteritidis. In conclusion, whey protein and lactic acid bacteria from Sumba mare’s milk have significant antimicrobial potentials during the treatment of Salmonellosis and Staphylococcus aureusinfections.
GROSS PATHOLOGICAL FINDINGS OF AFRICAN SWINE FEVER SUSPECTS IN OEBELO, KUPANG REGENCY, 2021 Maxs U. E. Sanam; Maria Aega Gelolodo; Larry R. W. Toha; Tri Utami; Yohanes T. R. M. R. Simarmata; Theresia F. I. M. D. Murni
JURNAL KAJIAN VETERINER Vol 9 No 3 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i3.7869

Abstract

African swine fever (ASF) is a destructive re-emerging swine disease that has posed a serious economic threat to the global pig farming sector. In past years, ASF has rapidly spread over Europe, Asia, and Oceania, and begin to enter Indonesia in the middle of 2019. The clinical and pathological symptoms of ASF are influenced by the strain's virulence, the transmission pathway, and the pig's immunological and health status. ASF’s clinical manifestations are known to evolve, from after an invasion enters a new free region to after the disease has been established in the territory for a longer period. Identifying ASF clinical signs and pathological changes is crucial for a comprehensive and reliable early detection system. The objective of this research is to observe and identify gross pathology in ASF suspect pigs in order to obtain a better understanding of the cause of death. Two dead pigs from a farm in Oebelo village, Kupang regency, Indonesia with a recent history of massive deaths had been examined in this study. The post-mortem results showed that hemorrhagic splenomegaly and hemorrhagic lymphadenitis were the main lesions observed at the examinations. Furthermore, hemorrhages were also found in various internal organs such as the kidneys, liver, and heart. To determine the exact cause of the pigs' deaths, a molecular diagnostic test should be conducted.
INTRODUCTION STUDY OF MDR STAPHYLOCOCCUS AUREUS IN PIG SLAUGHTER HOUSE IN KUPANG CITY Maxs U. E. Sanam; Novalino H. G. Kallau; Yeremia Y. Sitompul
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.7870

Abstract

Due to the unprudent use of antibiotics globally, it causes the incidence of antibiotic resistance with the emergence of bacteria that are resistant to antibiotics and has implications for the failure of the use of antibiotics in dealing with various cases of diseases caused by bacteria. The incidence of antibiotic resistance in the livestock sector occurs due to the use of antibiotics that are not based on recommendations and antibiotics are given in the long term. The presence of bacteria that carry resistance to one or several kinds of antibiotics in livestock is a threat to animal and human health. The city of Kupang as an area that has pig farms and a high pig population has a big challenge to the emergence of antibiotic resistance due to the use of antibiotics in maintaining livestock health. One of the bacteria that has a large number of distributions and is widely found in livestock and is an indicator of antibiotic resistance is Staphylococcus aureus (S. aureus). This research has used an observational research method based on data collection methods and laboratory examinations, which are adjusted to the objectives that have been set. The approach that has been used in this research is a Cross Sectional Study, because it wants to get an overview of S. aureus bacteria that are resistant to antibiotics. The sample that has been needed is pig feces obtained by field observations. The data analysis technique that has been used is a descriptive analysis model, to explain the presence of antibiotic-resistant S. aureus bacteria in pig farms. The results showed that this isolation and identification process resulted in 28 (51.85%) samples that were positive for S. aureus. The highest prevalence of resistance was found in S. aureus which was resistant to the antibiotic Colistin sulfate (82.1%). The number of antibiotics tested from S. aureus isolates showed various variations from 0 to 3 types of antibiotics that were resistant in 1 isolate. The most common resistance pattern shown in S. aureus isolates was the CS (Colistin sulfate) pattern as many as 19 of the 28 samples. The conclusion of this study encourages the need to increase supervision related to the use of antibiotics and increase public awareness of the use of antibiotics and prevention of the increasing incidence of antibiotic resistance.
IDENTIFIKASI MORFOLOGI LARVA Anisakis sp PADA Epinephelus sp. DAN Rastrelliger sp DI PERAIRAN NTT Maxs U. E. Sanam; Annytha Detha; Diana Wuri; Susana Dangga
JURNAL KAJIAN VETERINER Vol 9 No 2 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i2.8425

Abstract

The purpose of this research is to identify the level of intensity and degree of infection incidence of Anisakis sp in Rastrelliger sp and Epinephelus sp in eastern waters of Indonesia. A total of 190 fish specimens were randomized (random sampling) on ​​95 Rastrelliger sp and 95 Epinephelus sp from fish selling places in various areas of Kupang city. The larvae obtained were collected and fixed with 70% alcohol, and stained using Semichen acetic carmine solution. Morphological identification of Anisakis sp larvae through ventriculus, boring tooth, and mucron shapes on a stereo microscope. The parasites found were grouped into Anisakis sp type I and type II. Based on the results of the study, it was found that the presence of Anisakis sp. in Epinephelus sp that is 94 out of 95 fish. The results also confirmed that Anisakis sp infection implies a high risk of Epinephelus sp fish species so that further identification is needed at the molecular level.
INTENSITY LEVEL AND PREVALENCE OF Anisakis sp IN Epinephelus sp. AND Rastrelliger sp IN EAST INDONESIA Maxs U. E. Sanam; Annytha Detha; Diana Wuri; Susana Dangga
JURNAL KAJIAN VETERINER Vol 9 No 1 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i1.8426

Abstract

Anisakiasis adalah penyebab parasit zoonosis yang disebabkan oleh larva Anisakis sp. Manusia terinfeksi karena menelan ikan atau kerang mentah atau setengah matang yang mengandung Anisakis sp. Tujuan penelitian ini adalah untuk mengidentifikasi tingkat intensitas dan derajat kejadian infeksi Anisakis sp pada Rastrelliger sp dan Epinephelus sp di perairan timur Indonesia. Sebanyak 190 spesimen ikan diacak (random sampling) pada 95 Rastrelliger sp dan 95 Epinephelus sp dari tempat penjualan ikan di berbagai wilayah kota Kupang. Larva yang diperoleh dikumpulkan dan difiksasi dengan alkohol 70%, dan diwarnai menggunakan larutan semichen acetic carmine. Hasil ini memberikan informasi intensitas bahwa intensitas Anisakis sp pada Epinephelus sp dan Rastrelliger sp berturut-turut adalah 98% dan 3,15%. Derajat infeksi Anisakis sp. pada Epinephelus sp secara keseluruhan sebesar 7,80 termasuk kategori sedang, dan pada Rastrelliger sp sebesar 1,33 termasuk kategori rendah. Organ predileksi yang dominan pada ikan Epinephelus sp adalah organ otot, sedangkan pada ikan Rastrelliger sp predileksi yang dominan adalah usus. Hasil penelitian juga menegaskan bahwa infeksi Anisakis sp menyiratkan risiko tinggi spesies ikan Epinephelus sp sehingga diperlukan identifikasi lebih lanjut di tingkat molekuler.
Co-Authors Annytha Detha Diana Wuri Diana Wuri Kallau, Novalino Harold Geoffrey Larry R. W. Toha Maria Aega Gelolodo Susana Dangga Susana Dangga Theresia F. I. M. D. Murni Tri Utami Yeremia Y. Sitompul Yohanes T. R. M. R. Simarmata