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All Journal INDONESIAN JOURNAL OF PHARMACY Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Pharmaceutical and Clinical Research
Effendy De Lux Putra, Effendy De Lux
Departement of Phamacy Faculty of Sciences and Mathematics North Sumatra University Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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Journal : INDONESIAN JOURNAL OF PHARMACY

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QUANTITATIVE ANALYSIS OF GENERIC AND BRANDED NAME AMPICILLIN IN TABLETS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) Putra, Effendy De Lux
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.314 KB) | DOI: 10.14499/indonesianjpharm0iss0pp223-232

Abstract

The determination of ampicillin in tablets of branded and generic names by reversed phase high performance liquid chromatography (HPLC) had been carried out. Using column selectosphere C18 (25cm x 4,6 mm) as stationary phase; water : acetonitrile : potassium phosphate 1 M : acetic acid 1 N (909+80+10+1) V/V as mobile phase; flow rate = 2,5 ml/minute; sensitivity = 0,08 AUFS and uv detector at 254 nm wave length. Identification of ampicillin BPFI, ampicillin (Pharos), and ampicillin in tablets of branded and generic names got the retention time 5 minutes.Determination of calibration curve linearity showed a linear correlation between the peak area versus concentrations from 200 to 700 g/ml with the correlation coefficient, r = 0,9984 and got the equation of regression Y = 249,89 X - 4305,15. The recovery test of ampicillin (Pharos) showed the concentration 99,36%104,76% with deviation standard = 1,6447; relative error = 2,06% and coefficient of variation = 1,61%. The quantification of ampicillin in tablets with generic names showed the concentration = 99,69%104,99% (PT. Indofarma); 91,03%97,53% (PT. Phapros); 95,70%102, 44% (PT. Farma); and for tablets of branded names namely : Binotal (PT. Bayer) = 93,38%99,44%; Kalpicillin (PT. Kalbe Farma) = 91,41%97, 95%; Parpicilin (PT, Prafa) = 97,19%101,67% and Cetacilin (PT. Soho) = 95,60%98,58%. All samples fulfilled the requirement of Farmakope Indonesia fourth edition (1995) namely containing ampicillin not less than 90,0% and not more than 120,0% from the label declared.Key words : HPLC, Ampicillin, assay
Co-Authors Desyandri Desyandri Furi, Mustika Nilsya Febrika Zebua, Nilsya Febrika Siti Morin Sinaga Yuliasmi, Sri