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All Journal Biology, Medicine, & Natural Product Chemistry Journal of Islamic Pharmacy Al-Kimia Chemistry Indonesian Journal of Chemical Research PROFESSIONAL HEALTH JOURNAL
Eka Fitri Yanti
STIKes Harapan Bangsa
Biochemistry, Genetics & Molecular Biology Chemistry Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Biology, Medicine,

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The Effect of Pumpkin Fruit Ripeness (Cucurbita moschata. D) on Total Flavonoid Levels and Antioxidant Activity Linda Astutik; Eka Fitri Yanti
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.17-23

Abstract

Pumpkin fruit (Cucurbita moschata. D) belongs the Cucurbitaceae family which is a functional vegetable widely distributed in Indonesia because it has nutritional value and health benefits. This study aims to determine the value of total flavonoid content and antioxidant activity in the ethanol extract of unripe, mature and ripened pumpkin. Simplicia powder was extracted by maceration method using 96% solvent. Testing the total flavonoid content with the addition of AlCl3 at a wavelength of 425 nm and the antioxidant activity test was carried out using DPPH (1-1-diphenyl-2-picrylhydrazyl) as a free radical with a maximum wavelength of 515 nm using microplate reader. The results of the study concluded that the ethanolic extract of pumpkin flesh had a total flavonoid content of 0,146 mgQE/100g in unripe fruit, 0,221 mgQE/100g in mature pumpkin, 0,191 mgQE/100g in ripened pumpkin. The antioxidant activity of the ethanol extract of unripe, mature, and ripened pumpkin fruit obtained was not active or did not completely reduce free radicals.
Co-Authors Eviyanti Nazareth Larissa Tania Linda Astutik Lutvita Trisiwi Yuliatiningsih Mohammad Rofik Usman Novika Purwanti Rani Maharani Rani Maharani Yuana Dwi Agustin Zuhrotul Laili