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All Journal Biology, Medicine, & Natural Product Chemistry Journal of Islamic Pharmacy Al-Kimia Chemistry Indonesian Journal of Chemical Research PROFESSIONAL HEALTH JOURNAL
Eka Fitri Yanti
STIKes Harapan Bangsa
Biochemistry, Genetics & Molecular Biology Chemistry Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Al-Kimia

 1 
Sintesis Heptapeptida Linear (H-Tyr-Asp-Pro-Ala-Pro-Pro-Pro-Oh) dengan Menggunakan Dic/Oksima sebagai Reagen Pengkopling Rani Maharani; Eka Fitri Yanti
Al-Kimia Vol 4 No 1 (2016): June
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.099 KB) | DOI: 10.24252/al-kimia.v4i1.1449

Abstract

A tetrapeptide, an analog of  trypsin-modulating oostatic factor (TMOF), has been synthesised by using a method of solid-phase peptide synthesis on 2-chlorotrityl chloride resin. Fmoc strategy was applied on the synthesis. The formation of peptide bond was facilitated by DIC/oxime as coupling reagent. After all of amino acids were attached on the resin, tetrapeptidyl resin was added by a mixture of TFA:water:EDT(90:5:5) in dichloromethane to cleave the peptide.Crude peptide was purified by reverse-phase column chromatography and the purified peptide was analysed by TOF ES-MS spectroscopy.
Co-Authors Eviyanti Nazareth Larissa Tania Linda Astutik Lutvita Trisiwi Yuliatiningsih Mohammad Rofik Usman Novika Purwanti Rani Maharani Rani Maharani Yuana Dwi Agustin Zuhrotul Laili