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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia
Kustiariyah Tarman
Departemen Teknologi Hasil Perikanan, IPB University
Agriculture, Biological Sciences & Forestry

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Ekstraksi dan karakterisasi glukosamin dari cangkang udang vaname (Litopenaeus vannamei) dengan metode hidrolisis bertekanan: Extraction and characterization of glucosamine from vannamei shrimp (litopenaeus vannamei) shell using pressure hydrolysis method Muhammad Sujatmiko; Mala Nurilmala; Kustiariyah Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 2 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26(2)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i2.45668

Abstract

Glucosamines belong to the group of amino sugars and act as a precursor for the biosynthesis of glycosylated proteins and lipids in the body. The chemical, biological, and fermented extraction of glucosamine has not yet yielded optimal results. Pressurized hydrolysis treatment is needed to increase the yield of glucosamine. This study aimed to determine the characteristics of pressurized acid glucosamine extract from vannamei shrimp shell. The characteristics observed included the chemical composition, yield, degree of deacetylation, spectrum of functional groups, heavy metal contamination, and appearance. The chemical and heavy metal composition indicate the quality of shrimp shell are of standard allowed. Chitosan had a moisture content of 4.62%, ash content of 0.31%, protein content of 3.03%, deacetylation degree of 78.44%, viscosity of 222 cPs, flaky appearance, brownish-white color, and odorless. Pressurized acid extraction yielded white brownish glucosamine powder. The highest glucosamine yield was found in the 5% HCl acid extraction treatment at a pressure of 0.45 atm. The best glucosamine was obtained from the combined treatment of acid and pressure with the yield and degree of deacetylation that complied with the quality requirements of glucosamine.
Isolasi heparin dari jeroan ikan tuna (Thunnus sp.) : Isolation of heparin from tuna viscera (Thunnus sp.) Safrina Dyah Hardiningtyas; Hanin Bela Kartika; Kustiariyah Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 3 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26 (3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i3.46634

Abstract

Tuna is a valuable commodity in Indonesia and its production value has been increasing annually. Tuna processing produces 50-70% waste. The use of viscera waste in the production of novel biomaterials, including heparin, has been documented. The objective of this study was to obtain heparin from tuna viscera, specifically from the liver, stomach, intestine, and pyloric caeca. Heparin extraction was accomplished using an enzymatic technique utilizing papain. Crude heparin extract was subjected to acetone fractionation for further purification. The functional groups of pure heparin were analyzed using a Fourier Transform Infrared (FTIR) spectrophotometer, while the heparin content was determined using a sulfate glycosaminoglycan (GAG) assay. The findings of the study indicated that the intestinal and pyloric caeca had a heparin content of 43% and yielded 0.66±0.06%. Pure heparin tuna viscera comprise various functional groups, including carboxyl, acetyl, hydroxyl, ester cycles, and sulfated N atoms. This research indicates that tuna viscera has the potential to serve as a substitute for heparin, a commonly utilized medical resource.
Co-Authors Hanin Bela Kartika Mala Nurilmala Muhammad Sujatmiko safrina dyah hardiningtyas