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Pengaruh Ekstrak Metanol Akar Pasak Bumi (Eurycoma Longifolia, Jack.) terhadap Jumlah Splenosit Mencit Diinfeksi Listeria monocytogenes Darmawati, Idiani; HNES, Marsetyawan; Herwiyanti, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2011)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v11i2.935

Percobaan ini dirancang untuk mempelajari pengaruh ekstrak metanol akar E. longifolia, Jack (MEEL) pada respon imun spesifik dengan penekanan khusus pada jumlah splenoctyes. Desain penelitian adalah posttest only control group design. Empat puluh delapan tikus betina Balb / c yang digunakan di seluruh. Empat puluh delapan tikus yang digunakan berpikir studi, dan dibagi menjadi 4 kelompok. Kelompok pertama sebagai kontrol hanya menerima aquadest. Kelompok II, III, dan IV diberikan 100, 200 , dan 400 mg / kgBB per hari, masing-masing yang MEEL diberikan bersama 14 hari. Kemudian, pada hari-15, diinfeksi Listeria monocytogenes. Observasi dilakukan pada hari 0, 3, dan 10 setelah infeksi. Jumlah splenocytes layak diukur dengan MTT Assay. Data dianalisis menggunakan Anova satu jalan. Hasil penelitian menunjukkan bahwa ekstrak metanol E. longifolia, Jack pada 200 mg / kg BB per hari sejauh 14 hari dapat meningkatkan persentase splenocytes, dan puncaknya terdeteksi pada hari-0 infeksi, pada Kii 11.363% yaitu, pada hari-3 setelah infeksi di KIII, yaitu 10.433% dan pada hari-10 di KIII yaitu 8.907%. Uji Anova menunjukkan perbedaan bermakna dengan pengecualian kelompok I dengan IV, dan II dengan III dan III dengan IV. Disimpulan ekstrak metanol E. longifolia, Jack. root mampu meningkatkan persentase splenocytes di Balb / c tikus yang terinfeksi dengan L. monocytogenes.

Pengaruh Ekstrak Metanol Akar Pasak Bumi (Eurycoma Longifolia, Jack.) terhadap Jumlah Splenosit Mencit Diinfeksi Listeria monocytogenes Darmawati, Idiani; HNES, Marsetyawan; Herwiyanti, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2011)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v11i2.935

Percobaan ini dirancang untuk mempelajari pengaruh ekstrak metanol akar E. longifolia, Jack (MEEL) pada respon imun spesifik dengan penekanan khusus pada jumlah splenoctyes. Desain penelitian adalah posttest only control group design. Empat puluh delapan tikus betina Balb / c yang digunakan di seluruh. Empat puluh delapan tikus yang digunakan berpikir studi, dan dibagi menjadi 4 kelompok. Kelompok pertama sebagai kontrol hanya menerima aquadest. Kelompok II, III, dan IV diberikan 100, 200 , dan 400 mg / kgBB per hari, masing-masing yang MEEL diberikan bersama 14 hari. Kemudian, pada hari-15, diinfeksi Listeria monocytogenes. Observasi dilakukan pada hari 0, 3, dan 10 setelah infeksi. Jumlah splenocytes layak diukur dengan MTT Assay. Data dianalisis menggunakan Anova satu jalan. Hasil penelitian menunjukkan bahwa ekstrak metanol E. longifolia, Jack pada 200 mg / kg BB per hari sejauh 14 hari dapat meningkatkan persentase splenocytes, dan puncaknya terdeteksi pada hari-0 infeksi, pada Kii 11.363% yaitu, pada hari-3 setelah infeksi di KIII, yaitu 10.433% dan pada hari-10 di KIII yaitu 8.907%. Uji Anova menunjukkan perbedaan bermakna dengan pengecualian kelompok I dengan IV, dan II dengan III dan III dengan IV. Disimpulan ekstrak metanol E. longifolia, Jack. root mampu meningkatkan persentase splenocytes di Balb / c tikus yang terinfeksi dengan L. monocytogenes.

INHIBITORY ACTIVITY OF SNAIL (Achatina fulica (Lam.) Bowdich) MUCUS ON GROWTH OF MAMMARY CANCER IN RAT INDUCED WITH DMBA (7,12-DIMETHYLBENZ(α)ANTHRACENE) Aan Edison; Phebe Hendra; B. Boy Rahardjo Sidharta; Sri Herwiyanti; Christy Jacub
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 18, No 2 (2021)
Publisher : Sanata Dharma University

Back to nature as a medication concept has been accepted widely because it has fewer side effects than modern medicines. Researches on natural products as anticancer agent therapies are in progress. This present research was conducted to determine that the inhibitory activity of snail (Achatina fulica (Lam.) Bowdich) mucus inhibits the growth rate of mammary cancer in SpragueDawley rats. Five groups of female rats, with four individuals each, were induced with 7,12-dimethylbenz(α)anthracene (DMBA) for five weeks. Snail mucus was applied every seven days to the treated rats with three different dosages (15, 20, and 25 mg/kg BW). Observations were done on diameters and growth rate of the mammary cancer lump developed at the end of week 8, 10, 12, 14, and 16. Histopathological examination was carried out at the end of the 16th week. Inhibitory activity results showed a row of average diameter of the cancerous lumps on rats with the following details. The results obtained from the application of snail mucus at dosage of 15, 20, and 25 mg/kg BW were at 0.40, 0.60, and 0.09 cm respectively along with average growth rate of cancerous lumps at 1.50, 0.75, and 0.25. The histopathology results of the snail mucus treatment at dosage of 15, 20, and 25 mg/kg BW showed normal tissue depiction and similarly normal histopathological form indicated by the presence of their sub-clinic components. The results showed that a snail mucus dose of 25 mg/kg BW was able to perform inhibitory activity on growing mammary cancer in rats induced by DMBA.

The relationship between sirtuin 1 (SIRT1) expression and tumor size, Proliferating Cell Nuclear Antigen (PCNA) expression and histological grading in rat breast carcinoma induced by dimethylbenz()anthracene (DMBA) Novrita Padauleng; Dewajani Purnomosari; Sri Herwiyanti; . Harjadi; . Irianiwati; Sitarina Widyarini
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 04 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Controversy regarding the role of SIRT1 in pathology of cancers exists and is still under debate.SIRT1 could act as either a tumor supressor or tumor promotor. This study was conducted toevaluate the relationship between SIRT1 expression and tumor size, Proliferating Cell NuclearAntigen (PCNA) expression and histological grading in rat breast carcinoma induced bydimethylbenz(á)anthracene (DMBA). Thirty female Sprague Dawley rats were randomly allocatedinto three groups with 10 rats in each group. Group 1 as negative control was just fed thestandard food. Group 2 as vehicle control was fed the standard food and corn oil. Group 3 asinduction group was fed the standard food and induced with DMBA at dose of 20 mg/kg bodyweight (BW) in corn oil twice a week for five weeks. All rats were palpated weekly to determinethe appearance, size and location of tumors. Sixteen weeks after DMBA induction rats weresacrified and histological preparations of the breast carcinoma tissue were then processed forSIRT1 and PCNA expression examination as well as histological grading. The result showed thatSIRT1 expression was significantly higher in breast carcinoma tissue compared to normal gland(26.12 vs 0.05; p = 0.004). SIRT1-positive was observed mostly in poor histological gradecarcinomas (56.2%), and it was not observed in good histological grade carcinomas. However,there was no significantly difference between SIRT1 and histological grading (p = 0.097; r =0.285). A significant correlation between SIRT1 expression and the tumor size (p =0.009; r=0.877), as well as PCNA expression (p =0.000; r =0.790) was observed. In conclusion, thereis relationship between SIRT1 expression and tumor size as well as PCNA expression in rat breastcarcinoma induced by DMBA.

EFFECT OF 1,2-EPOXY-3[3-3[3,4-DIMETOXYI-PHENIL]-4H-1-BENZOPIRAN-4-ON] PROPANE (EPI) ON SIRTUIN-1 AND NUCLEAR FACTOR-κB EXPRESSION OF MAMMARY TUMORS INDUCED IN SPRAGUE DAWLEY RATS BY DMBA Ayyub Harly Nurung; Sri Herwiyanti; Dewi Kartikawati Paramita
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 3 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACT The main factors contribute to breast cancer development is the combination of exogenous and endogenous factors. Endogenous factors include both SIRT1 and NF-kB. Exogenous factor used in this research is 7.12 dimethylbenz(a)anthracene (DMBA). 1,2-epoxy-3[3- 3[3,4-dimetoxy-phenil]-4h-1-benzophiran-4-on] propane (EPI) is a derivative of isoflavone generate from clove leaf oil. To examine the effect of EPI on SIRT1 and NF-kB expression in DMBA-induced Sprague Dawley (SD) rats, and the correlation between SIRT1 and NFkB expressions. Tissue generated form 35 Sprague Dawley female rats aged 2 weeks old were used in this study. Those rats were divided into 7 groups (5 rats/group), namely normal control group; corn oil group; DMBA group; EPI treated groups with 1 mg/kgBW (EPI I), 2 mg/kgBW (EPI II), and 4 mg/kgBW (EPI III), respectively; and doxorubicin group. EPI and doxorubicin were administered from 1st until 15th week, while DMBA were administered from 3rd until 9th week. The paraffin block was prepared from all breast organ of the rats that terminated at the end of week 15th. Examination of SIRT1 and NF-kB expression was performed using light microscope at 400x magnifications, after immunohistochemistry (IHC) staining. Expression level of SIRT1 and NF-kB were analyzed using IHC-profiler plug-in in ImageJ software. SIRT1 and NF-kB expression in EPI treated groups were not significantly different with the one in Doxorubicin group, but lower than DMBA group (p=0.000). There was a positive correlation between SIRT1 and NF-kB expression (p=0.001; r=0.773) in EPI-treated group. EPI was able to prevent an increasing of SIRT1 and NF-kB expression in SD model breast cancer that induced with DMBA. There is a positive correlation between SIRT1 and NF-kB expression in EPI-treated SD rats that were induced by DMBA

The effects of ethanolic extract of Phaleria macrocarpa (Scheff.) Boerl leaf on macrophage phagocytic activity in diabetic rat model Ira Cinta Lestari; Muhammad Ghufron; Sri Herwiyanti; Yustina Andwi Ari Sumiwi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 2 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Diabetic patients suffer inflammation and immune deficiency as a consequence of the decrease in macrophage phagocytic activity, thus making them vulnerable to infection. The ability of Ethanolic Extract of Phaleria macrocarpa Leaf (EEPML) to increase macrophage phagocytic activity has also a potential in the diabetic case. EEPML also has anti-inflammatory effect. In this study the EEPML potential to increase peritoneal macrophage phagocytic activity and change M1 and M2 macrophage percentage in diabetic rat model is investigated. This was a quasi experimental study with post test only control group design. Fourty five male Sprague Dawley rats within the age of 8 weeks were classified into normal control group, diabetic control group with solvent, diabetic with 7mg/200g, 14mg/200g, and 28mg/200g of EEPML peroral administration, once a day. The diabetic rat model was made with streptozotocin and nicotinamide injection. The rats were terminated in 3rd, 14th and 25th day of extract administration. Peritoneal fluid was isolated then cultured for macrophage phagocytic activity assay with latex beads. M1 and M2 macrophage percentage was analyzed using flow cytometry with anti CD40 and CD206 antibody. Result of statistical analysis show that active macrophage and phagocytic index mean of EEPML rat groups on day 3, 14 and 25 was significantly higher than the control group. The mean of M1 macrophage percentage of EEPML rat groups was significantly higher than control on day 3 and 14, and lower on day 25, while mean of M2 macrophage percentage didn’t show any significant difference within groups. Conclusion of this study is administration of EEPML increases peritoneal macrophage phagocytic activity on day 3, 14 and 25. This is also increases M1 macrophage percentage on day 14, decrease M1 macrophage percentage on day 25, and doesn’t change peritoneal M2 macrophage percentage in diabetic rat model.

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