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All Journal JURNAL ILMIAH PLATAX
Rizald M. Rompas
Sam Ratulangi University

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Application of chlorophyll in carrageenan from algae Kappaphycus alvarezii (Doty) Doty 1996 Budi Kurniawan; Desy M. H. Mantiri; Kurniati Kemer; Rizald M. Rompas; Nickson J. Kawung; Joppy D. Mudeng
Jurnal Ilmiah PLATAX Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.41935

Abstract

The aim of this study is to determine the effect of NaOH and KOH on carrageenan. The addition of natural dyes was carried out with different concentrations of carrageenan and the value of viscosity and gel strength of the algae Kappaphycus alvarezii to a mixture of natural dyes. The samples of algae were taken from the cultivation area in Belang waters, Southeast Minahasa Regency. The results of this study on the addition of natural dyes in refined carrageenan have succeeded in giving a green color and did not affect the viscosity and gel strength of the carrageenan gel when compared to the control (without treatment). The viscosity value of carrageenan with 4% NaOH concentration was 53.34-53.69 cP and KOH concentration was 49.55-50.03 cP. The gel strength value at 4% NaOH concentration was 74.11-74.89 mm/g/sec, while at 5% KOH concentration it was 84.22-84.89 mm/g/sec. The viscosity and gel strength still meet standards set by the Food Agriculture Organization (FAO).Keywords: Carrageenan, natural dye, Kappaphycus alvarezii, viscosity, gel strengthAbstrakPenelitian ini bertujuan untuk mengetahui pengaruh NaOH dan KOH terhadap karagenan. Pada penelitian ini dilakukan penambahan pewarna alami dengan konsentrasi yang berbeda terhadap refined carrageenan dan nilai viskositas serta kekuatan gel karagenan dari alga Kappaphycus alvarezii terhadap campuran pewarna alami. Sampel alga diambil dari area budidaya di Perairan Belang, Kabupaten Minahasa Tenggara. Hasil penelitian tentang penambahan pewarna alami pada refined carrrageenan telah berhasil memberikan warna hijau dan tidak mempengaruhi viskositas serta kekuatan gel karagenan jika dibandingkan dengan kontrol (tanpa perlakuan). Nilai viskositas karagenan dengan konsentrasi NaOH 4% adalah 53,34 – 53,69 cP dan konsentrasi KOH 5% sebesar 49,55 – 50,03 cP. Nilai kekuatan gel pada konsentrasi NaOH 4% sebesar 74,11-74,89 mm/g/det, sedangkan pada konsentrasi KOH 5% diperoleh 84,22-84,89 mm/g/det. Viskositas dan kekuatan gel tersebut masih memenuhi standar yang ditetapkan oleh Food Agriiculture Organization (FAO).Kata kunci: Karagenan, pewarna alami, Kappaphycus alvarezii, viskositas, kekuatan gel
Effect Of Lead Acetate (Pb(Ch3coo)2 On The Growth Of Marine Microalgaes Chlorella vulgaris (Beijerink, 1890) Aprilisa Viony Jeheskiel; Kurniati Kemer; Desy Maria Helena Mantiri; James Paulus; Rizald M. Rompas; Renny Khreekhoff
Jurnal Ilmiah PLATAX Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42458

Abstract

Microalgae are single-celled microorganisms, forming colonies and are very commonly found in large waters such as seas, lakes, rivers, and swamps. The purpose of this study was to determine the growth and density of microalgae Chlorella vulgaris in a controlled container with the administration of lead acetate at different concentrations. Cell growth of Chlorella vulgaris with samples of marine microalgae Chlorella vulgaris from culture containers. At the beginning of the exponential phase, the microalgae were given lead acetate in 3 containers with concentrations of 30 ppm, 50 ppm, 80 ppm, and control/without treatment. The results showed that the growth of Chlorella vulgaris cells with lead acetate administration experienced unstable growth compared to those without lead acetate administration (control).Keywords: Microalgae; Chlorella vulgaris; Culture; Lead AcetateAbstrakMikroalga merupakan mikroorganisme bersel satu, membentuk koloni dan sangat banyak dijumpai di perairan besar seperti pada laut, danau, sungai serta perairan payau. Tujuan dari penelitian ini adalah untuk mengetahui pertumbuhan dan kepadatan mikroalga  Chlorella vulgaris dalam wadah terkontrol dengan pemberian timbal asetat pada konsentrasi yang berbeda. Pertumbuhan sel Chlorella vulgaris denga Sampel mikroalga laut Chlorella vulgaris berasal dari wadah kultur. Pada awal fase eksponensial, mikroalga diberikan timbal asetat ke dalam 3 wadah dengan konsentrasi 30 ppm, 50 ppm, 80 ppm serta kontrol/tanpa perlakuan. Hasil penelitian menunjukkan bahwa pertumbuhan sel Chlorella vulgaris dengan pemberian timbal asetat mengalami pertumbuhan yang tidak stabil dibandingkan tanpa pemberian timbal asetat (kontrol).Kata kunci: Mikroalga;  Chlorella vulgaris;  Kultur; Timbal Asetat