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Desy Maria Helena Mantiri
Sam Ratulangi University
Environmental Science

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Phycoeritryn Pigments In Carrageenan From Algae Kappaphycus alvarezii (Doty) Doty 1996 Abigael Joanete Tumalun; Desy Maria Helena Mantiri; Darus Sa'adah Johanis Paransa; Rizald Max Rompas; Kurniati Kemer; Joppy Mudeng
Jurnal Ilmiah PLATAX Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42456

Abstract

Kappaphycus alvarezii is a marine alga cultivated in Indonesia and produces carrageenan. Carrageenan is a product used in the cosmetic, food, and pharmaceutical industries. This study aimed to test the viscosity and gel strength of refined carrageenan with the addition of natural dye phycoerythrin from the algae Halymenia durvillei with different concentrations. Refine carrageenan was made using alkaline solvents of 4% NaOH and 5% KOH and then boiled using a pressure cooker. The results showed a reddish color change in the pure carrageenan refinement with the addition of phycoerythrin pigment, especially at a concentration of 50%. The average value of refined carrageenan viscosity for 4% NaOH concentration was 49.22 – 50.27 cP, and 5% KOH concentration ranged from 47.16 – 50.12 cP. Testing the gel strength of the refined carrageenan, the average NaOH 4% was 72.67 – 82.00 mm/g/sec, and the 5% KOH concentration was 81.00 – 81.78 mm/g/sec. The addition of phycoerythrin pigment to refine carrageenan had no effect on viscosity and gel strength. Drying at a temperature of 100oC obtained a water content of semi-refined carrageenan between 2.91 - 4.38%, this value is in accordance with the standard for carrageenan water content from FAO, which is a maximum of 12%.Keywords: Carrageenan, phycoerythrin pigments, Kappaphycus alvarezii, Halymenia durvillei, viscosity, gel strength.AbstrakKappaphycus alvarezii merupakan alga laut yang dibudidayakan di Indonesia dan penghasil karagenan. Karagenan merupakan suatu produk yang digunakan dalam industri kosmetik, makanan, dan juga farmasi. Tujuan dari penelitian adalah menguji viskositas dan kekuatan gel terhadap karagenan refine yang ditambahkan pigmen fikoeritrin dari alga Halymenia durvillei dengan perbedaan konsentrasi. Karagenan refine dibuat dengan menggunakan pelarut alkali NaOH 4% dan KOH 5% selanjutnya direbus dengan tekanan tinggi. Hasil yang didapat menunjukan bahwa terjadi perubahan warna kemerahan pada karagenan refine dengan penambahan pigmen fikoeritrin, terutama pada konsentrasi 50%. Nilai rata-rata viskositas karagenan refine untuk konsentrasi NaOH 4% adalah 49.22 – 50.27 cP, dan konsentrasi KOH 5% berkisar 47.16 – 50.12 cP. Pengujian kekuatan gel pada karagenan refine diperoleh rata-rata NaOH 4% sebesar 72.67 – 82.00 mm/g/det dan konsentrasi KOH 5% adalah 81.00 – 81.78 mm/g/det. Penambahan pigmen pada karagenan refine tidak berpengaruh pada viskositas dan kekuatan gel. Pengeringan dengan suhu 100OC memperoleh kadar air pada karagenan semi refine antara 2.91 - 4.38%, nilai tersebut telah sesuai dengan standar kadar air karagenan dari FAO yaitu maksimum 12%.Kata kunci: Karagenan, pigmen fikoeritrin, Kappaphycus alvarezii, Halymenia durvillei, viskositas, kekuatan gel. 
Effect Of Lead Acetate (Pb(Ch3coo)2 On The Growth Of Marine Microalgaes Chlorella vulgaris (Beijerink, 1890) Aprilisa Viony Jeheskiel; Kurniati Kemer; Desy Maria Helena Mantiri; James Paulus; Rizald M. Rompas; Renny Khreekhoff
Jurnal Ilmiah PLATAX Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42458

Abstract

Microalgae are single-celled microorganisms, forming colonies and are very commonly found in large waters such as seas, lakes, rivers, and swamps. The purpose of this study was to determine the growth and density of microalgae Chlorella vulgaris in a controlled container with the administration of lead acetate at different concentrations. Cell growth of Chlorella vulgaris with samples of marine microalgae Chlorella vulgaris from culture containers. At the beginning of the exponential phase, the microalgae were given lead acetate in 3 containers with concentrations of 30 ppm, 50 ppm, 80 ppm, and control/without treatment. The results showed that the growth of Chlorella vulgaris cells with lead acetate administration experienced unstable growth compared to those without lead acetate administration (control).Keywords: Microalgae; Chlorella vulgaris; Culture; Lead AcetateAbstrakMikroalga merupakan mikroorganisme bersel satu, membentuk koloni dan sangat banyak dijumpai di perairan besar seperti pada laut, danau, sungai serta perairan payau. Tujuan dari penelitian ini adalah untuk mengetahui pertumbuhan dan kepadatan mikroalga  Chlorella vulgaris dalam wadah terkontrol dengan pemberian timbal asetat pada konsentrasi yang berbeda. Pertumbuhan sel Chlorella vulgaris denga Sampel mikroalga laut Chlorella vulgaris berasal dari wadah kultur. Pada awal fase eksponensial, mikroalga diberikan timbal asetat ke dalam 3 wadah dengan konsentrasi 30 ppm, 50 ppm, 80 ppm serta kontrol/tanpa perlakuan. Hasil penelitian menunjukkan bahwa pertumbuhan sel Chlorella vulgaris dengan pemberian timbal asetat mengalami pertumbuhan yang tidak stabil dibandingkan tanpa pemberian timbal asetat (kontrol).Kata kunci: Mikroalga;  Chlorella vulgaris;  Kultur; Timbal Asetat
Morphological Characteristics and Shell Color Of Littoraria pallescens Prosobrancia Molusca From Different Mangrove In Tongkaina Waters, Manado City Vellysa Friendly Salawati; Desy Maria Helena Mantiri; Farnis Bineada Boneka; Noldy Gustaf Frans Mamangkey; Veibe Warouw; Ockstan Kalesaran
Jurnal Ilmiah PLATAX Vol. 10 No. 1 (2022): ISSUE JANUARY-JUNE 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i1.38559

Abstract

The purpose of this study was to identify sea slugs L. pallescens taken from two different mangroves, namely Rhizophora mucronata and Avicennia marina in Tongkaina waters, Bunaken District, Manado City based on morphology and anatomy as well as shell color. Identification of mangroves and sea slugs refers to the identification book. The results obtained were L. pallescens species with elongated and tapered morphology at the end of the shell measuring 0.3-2.7 cm. The operculum is purple. The color of the shell obtained was 66.85% consisting of dark colors (black, black, orange, brown and gray spots), occupying the stems and roots of the mangrove, while the light colors (yellow, yellow, dark spots and red) were found to be 33.15%, occupying the leaves and stems of mangroves. The high survival rate of L. pallescens was found in the mangrove roots. This species was found in R. mucronata by 65.26% while in A. marina only 34.74%, this could be caused by differences in the shape of the mangrove roots.Keywords: L. pallescens; Mangrove; Shell color; MorphologyAbstrakTujuan penelitian ini adalah mengindentifikasi siput laut L. pallescens yang diambil dari dua mangrove berbeda yaitu Rhizopora mucronata dan Avicennia marina di perairan Tongkaina, Kecamatan Bunaken, Kota Manado berdasarkan morfologi dan anatomi serta warna cangkang. Identifikasi mangrove dan siput laut merujuk pada buku identifikasi. Hasil penelitian yang diperoleh yaitu spesies L. pallescens dengan bentuk morfologi memanjang dan meruncing, pada bagian ujung cangkang berukuran  0,3-2,7 cm. Operculum berwarna ungu. Warna cangkang yang diperoleh 66,85% yang terdiri dari warna gelap (hitam, hitam bercak orange, coklat dan abu-abu), menempati bagian batang dan akar mangrove sedangkan warna terang (kuning, kuning bercak gelap dan merah) didapat 33,15%, menempati bagian daun dan batang mangrove. Tingginya kelangsungan hidup  L. pallescens berada pada bagian akar mangrove. Spesies ini ditemukan pada R. mucronata sebesar 65,26% sedangkan pada A. marina hanya 34,74%, hal ini dapat disebabkan oleh karena perbedaan bentuk akar mangrove.  Kata Kunci: L. Pallescens; Mangrove; Warna cangkang; Morfologi
Co-Authors Abigael Joanete Tumalun Aprilisa Viony Jeheskiel Darus Sa'adah Johanis Paransa Farnis Bineada Boneka James Paulus Joppy Mudeng Kurniati Kemer Noldy Gustaf Frans Mamangkey Ockstan Kalesaran Renny Khreekhoff Rizald M. Rompas Rizald Max Rompas Veibe Warouw Vellysa Friendly Salawati