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All Journal JURNAL ILMIAH PLATAX
Nickson J. Kawung
Sam Ratulangi University

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Application of chlorophyll in carrageenan from algae Kappaphycus alvarezii (Doty) Doty 1996 Budi Kurniawan; Desy M. H. Mantiri; Kurniati Kemer; Rizald M. Rompas; Nickson J. Kawung; Joppy D. Mudeng
Jurnal Ilmiah PLATAX Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.41935

Abstract

The aim of this study is to determine the effect of NaOH and KOH on carrageenan. The addition of natural dyes was carried out with different concentrations of carrageenan and the value of viscosity and gel strength of the algae Kappaphycus alvarezii to a mixture of natural dyes. The samples of algae were taken from the cultivation area in Belang waters, Southeast Minahasa Regency. The results of this study on the addition of natural dyes in refined carrageenan have succeeded in giving a green color and did not affect the viscosity and gel strength of the carrageenan gel when compared to the control (without treatment). The viscosity value of carrageenan with 4% NaOH concentration was 53.34-53.69 cP and KOH concentration was 49.55-50.03 cP. The gel strength value at 4% NaOH concentration was 74.11-74.89 mm/g/sec, while at 5% KOH concentration it was 84.22-84.89 mm/g/sec. The viscosity and gel strength still meet standards set by the Food Agriculture Organization (FAO).Keywords: Carrageenan, natural dye, Kappaphycus alvarezii, viscosity, gel strengthAbstrakPenelitian ini bertujuan untuk mengetahui pengaruh NaOH dan KOH terhadap karagenan. Pada penelitian ini dilakukan penambahan pewarna alami dengan konsentrasi yang berbeda terhadap refined carrageenan dan nilai viskositas serta kekuatan gel karagenan dari alga Kappaphycus alvarezii terhadap campuran pewarna alami. Sampel alga diambil dari area budidaya di Perairan Belang, Kabupaten Minahasa Tenggara. Hasil penelitian tentang penambahan pewarna alami pada refined carrrageenan telah berhasil memberikan warna hijau dan tidak mempengaruhi viskositas serta kekuatan gel karagenan jika dibandingkan dengan kontrol (tanpa perlakuan). Nilai viskositas karagenan dengan konsentrasi NaOH 4% adalah 53,34 – 53,69 cP dan konsentrasi KOH 5% sebesar 49,55 – 50,03 cP. Nilai kekuatan gel pada konsentrasi NaOH 4% sebesar 74,11-74,89 mm/g/det, sedangkan pada konsentrasi KOH 5% diperoleh 84,22-84,89 mm/g/det. Viskositas dan kekuatan gel tersebut masih memenuhi standar yang ditetapkan oleh Food Agriiculture Organization (FAO).Kata kunci: Karagenan, pewarna alami, Kappaphycus alvarezii, viskositas, kekuatan gel
Analysis of Carotenoid Pigment Types in the Carapace of the Male Crab Grapsus albolinetaus Latreille in Milbert 1812 Fatika Sari Mokoginta; Darus Saadah Johanis Paransa; Kurnati Kemer; James J. H. Paulus; Nickson J. Kawung; Henky Manoppo
Jurnal Ilmiah PLATAX Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34421

Abstract

Carotenoid pigments have various colors such as yellow, orange, or red-orange. One of the carotenoid pigment sources is Crab G. albolineatus Latreille in Milbert 1812. Column chromatography separation technique was used to determine the metabolism of carotenoid pigments in the crab G. albolineatus latreille in Milbert 1812. This CC separation used hexane and acetone as the developer solution (70:30). The developer solution as known as the mobile phase is semipolar, while the stationary phase is silica powder G60. Therefore, it formed two metabolic pathways. The male G. albolineatus crab used in this study was on the D3 molting stage which had a concentration of 36.37 g/g dry residue and 4.72 g content. The types of pigments identified are: β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Keywords: Carotenoid Pigments; G. albolineatus; Column Chromatography; MoltingAbstrakPigmen karotenoid memiliki berbagai warna seperti kuning, oranye, atau merah oranye. Salah satu sumber pigmen karotenoid adalah pada karapas kepiting G. albolineatus Latreille in Milbert 1812. Untuk mengetahui metabolisme jenis pigmen karotenoid pada kepiting Grapsus albolineatus latreille in Milbert 1812 yaitu menggunakan pemisahan kromatografi Kolom. Pemisahan KK ini menggunakan larutan pengembang heksan dan aseton (70:30). Larutan pengembang merupakan fase gerak yang bersifat semipolar dan fase diamnya menggunakan bubuk silika G60. Terbentuk dua  jalur metabolisme. Kepiting G. albolineatus jantan yang digunakan pada penelitian berada di stadium molting D3 dengan konsentrasi sebesar 36,37 µg/g berat residu kering dan kandungan 4,72 µg. Jenis pigmen yang teridentifikasi yaitu : β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Kata kunci: Pigmen; Karotenoid; G. albolineatus; Kromatofrafi Kolom; Molting