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Highly Sensitive Phenol Biosensor Utilizing Selected Bacillus Biofilm Through an Electrochemical Method Ariyanti, Dita; Iswantini, Dyah; Sugita, Purwantiningsih; Nurhidayat, Novik; Effendi, Hefni; Ghozali, Ali Aulia; Kurniawan, Yehezkiel Steven
Makara Journal of Science Vol. 24, No. 1
Publisher : UI Scholars Hub

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Abstract

An eco-friendly phenol biosensor from Bacillus biofilm was prepared and investigated. The biofilm, which produced tyrosinase enzyme, was successfully immobilized on a screen-printed carbon electrode surface. A total of 72 Bacillus isolates were utilized because of their capability to produce tyrosinase enzyme in tyrosine media. Among them, Bacillus isolate code 100 was selected because it produced an adequate amount of tyrosinase enzyme and a high potentiostat current. The response surface methodology was also used to optimize the phenol sensing condition through an electrochemical method. Results showed that the optimum condition was achieved after 6 days on a phosphate buffer solution (pH of 8), with an optical density of 0.33. Furthermore, the limits of detection and quantification were 3.0 and 13 ng/L, respectively. The measurements of precision yielded a relative standard deviation of < 5%, which is remarkable. Although the biosensor material was used for 35 days, the current throughout was still maintained at 90%, indicating that the evaluated biosensor material has the potential to be used for phenol monitoring on environmental samples in the near future.
MAKANAN TAMBAHAN IBU HAMIL UNTUK MENCEGAH STUNTING DALAM UPAYA MENINGKATKAN KUALITAS SDM BAGI PERTAHANAN NEGARA Arfiyanti; Dita Ariyanti
JURNAL KIMIA SAINTEK DAN PENDIDIKAN Vol. 6 No. 2 (2022): Jurnal Kimia Saintek dan Pendidikan
Publisher : Program Studi Kimia - Universitas Sari Mutiara Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/kimia.v6i2.3494

Abstract

Anak dengan stunting memiliki kemampuan verbal dan IQ rendah, disabilitas kognitif, penurunan kemampuan motorik dan gangguan fungsi imunitas. Perlu pemberian makanan tambahan (PMT) sebagai pendamping program suplementasi pemerintah pada ibu hamil untuk meningkatkan status gizi ibu hamil dan mencegah stunting dalam upaya meningkatkan kualitas SDM bagi pertahanan negara.  Mendapatkan formulasi fortifikasi cookies yang sesuai kebutuhan ibu hamil untuk mencegah stunting. Formulasi cookies berawal dari  pembuatan tepung (tempe, kacang hijau, ikan tuna, pisang), dilanjutkan dengan formulasi tepung  komposit. Rancangan metode optimasi tepung komposit RSM mixture design D-optimal, menggunakan software Design Expert 7.0 trial (DX 7 trial). Kandungan zat gizi bahan pangan yang digunakan lalu dibandingkan dengan jumlah zat gizi yang ingin ditambahkan. Formula tepung komposit dipilih berdasarkan kandungan gizi dan rasa. Substitusi tepung beras dengan tepung komposit terpilih pada resep  standar. Cookies berbentuk bulat, aroma, rasa, warna, kenampakan sesuai SNI01-2891-1992, BUTIR1.2. Kandungan protein, energi, vitamin A, B12, calsium, pospor, iodium, asam folat memenuhi AKG ibu hamil trimester II dan persyaratan BPOM. 70% ibu hamil trimester II menyukai cookies. Cookies ikan tuna dapat digunakan sebagai PMT ibu hamil pendamping program suplementasi pemerintah untuk mencegah stunting 180 HPK.