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All Journal Mandala of Health : A Scientific Journal
Anriani Puspita Karunia Ning Widhi
Universitas Jenderal Soedirman
Dentistry Health Professions Public Health

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UJI EFEKTIVITAS YOGHURT SINGLE DAN DOUBLE STRAIN DALAM MENGHAMBAT BAKTERI Salmonella typhi PENYEBAB DEMAM TIFOID Anriani Puspita Karunia Ning Widhi; Annisa Sekar Salsabila; Hajid Rahmadianto Mardihusodo; IDSAP Peramiarti
Mandala Of Health Vol 15 No 2 (2022): Mandala Of Health
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.519 KB) | DOI: 10.20884/1.mandala.2022.15.2.6420

Abstract

Salmonella typhi adalah bakteri gram negatif penyebab demam tifoid. Penyakit ini dapat dicegah dengan mengonsumsi makanan fermentasi yang mengandung bakteri asam laktat (BAL). Yoghurt merupakan salah satu produk olahan hasil fermentasi BAL dengan bahan dasar susu. BAL yang digunakan dalam pembuatan yoghurt adalah single strain Lactobacillus bulgaricus, Streptococcus thermophilus serta double strain Lactobacillus bulgaricus dan Streptococcus thermophilus. Penelitian ini bertujuan untuk mengetahui perbandingan BAL yoghurt single strain dengan double strain dalam menghambat pertumbuhan Salmonella typhi dengan menghitung rata-rata zona hambat yang terbentuk. Metode penelitian ini adalah experimental design dengan post test only with control group design. Pengujian bakteri menggunakan difusi sumuran. Total sampel sebanyak 16 sampel dibagi menjadi 4 kelompok konsentrasi masing-masing 0%, 20%, 40%, 60%, dan 80%. Rancangan percobaan pada penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL). Analisis data menggunakan uji Kruskal Wallis dan Post Hoc Mann Whitney. Hasil penelitian didapatkan median diameter zona hambat BAL yoghurt double strain Streptococcus thermophilus dan Lactobacillus bulgaricus > single strain Lactobacillus bulgaricus > Streptococcus thermophilus. Pada uji Kruskal Wallis didapatkan nilai p = 0,000, terdapat perbedaan antara kelompok yang diberi perlakuan dan tidak diberi perlakuan. Hasil uji penelitian didapatkan perbedaan signifikan median diameter zona hambat antar kelompok. Kesimpulan dari penelitian ini menunjukan bahwa yoghurt double strain memiliki kemampuan penghambatan pertumbuhan Salmonellla typhi yang lebih baik dibandingkan yoghurt single strain Lactobacillus Blugaricus atau Streptococcus thermophilus.
PREVALENCE OF (Aspergillus niger) IN OTOMYCOSIS AT PROF. DR. MARGONO SOEKARJO HOSPITAL Dinda Victoria; Anriani Puspita Karunia Ning Widhi; Darmawan Anton Budhi
Mandala Of Health Vol 16 No 1 (2023): Mandala of Health
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mandala.2023.16.1.8005

Abstract

Otomycosis is a chronic fungal infection with high prevalence. Environmental factors dan predisposing factors can influence a patient’s vulnerability to otomycosis. According to studies conducted in several country, Aspergillus niger is the common fungus that causes otomycosis. This study aims to determine the prevalence of Aspergillus niger in patients at Prof. dr. Margono Soekarjo Hospital, who was clinically diagnosed with otomycosis. The study was done at the ENT Poly of Prof. dr. Margono Soekarjo Hospital and Microbiology Laboratory of Medicine Faculty, Jenderal Soedirman University between August 2022 to Januari 2023 as a descriptive observational study. According to the inclusion criteria, there were 42 subjects and 46 samples that ENT specialists carried out with sterile flocked swabs, the isolated the samples bedside on SDA Chloramphenicol, Czapek Dox agar, and object glass. Samples were observed macroscopically and microscopically with 10% KOH and LPCB staining. Patients with Aspergillus niger infection in otomycosis at Prof. dr. Margono Soekarjo Hospital , are represented by nine (19,56%) out of the 46 samples. The gender with the highest sex precentage is female (55,56%), folllowed by the age range of 26 – 35 years (44,45%). The prevalence of Aspergillus niger as a cause of otomycosis in Prof. dr. Margono Soekarjo Hospital is 19,56%, the most in females and the age group 26 – 35 years.
PERBANDINGAN BAKTERI ASAM LAKTAT YOGHURT SINGLE DAN DOUBLE STRAIN DALAM MENGHAMBAT PERTUMBUHAN Shigella dysenteriae Bella Khoirunisa Prihandini; Anriani Puspita Karunia Ning Widhi; Nendyah Roestijawati
Mandala Of Health Vol 16 No 1 (2023): Mandala of Health
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mandala.2023.16.1.8397

Abstract

Dysentery is an infectious intestinal disease characterized by the appearance of blood in the stool. One of the prevention efforts is consuming fermented milk as yogurt. Yoghurt is a fermented milk using lactic acid bacteria (LAB). LAB used as single strains of Streptococcus thermophilus, Lactobacillus bulgaricus, and double strains of S. thermophilus and L. bulgaricus. The purpose of this study was to determine the ratio of LAB of single strain and double strain yoghurt in inhibiting the growth of Shigella dysenteriae by calculating the inhibition zone formed. This research used experimental design method with postest only with control group design. This study uses well diffusion. The total sample was 15, divided into 5 groups with 0%, 20%, 40%, 60%, dan 80% concentration respectively as controls. The experimental design in this study used the Completely Randomized Design (CRD) method. Data analysis used Kruskal Wallis test and Mann Whitney Post Hoc test. The results show that the average diameter of the LAB inhibition zone for double strains of S. thermophilus and L. bulgaricus > single strain L. blugaricus > S. thermophilus. In the Kruskal Wallis test, the p value was obtained = 0.002, it was found that the difference between the treated and untreated groups was obtained. The test results found a significant difference in the median inhibition zone diameter between groups. The conclusion of this study shows that the LAB double strains of S. thermophilus and L. bulgaricus are better at inhibiting growth S. dysenteriae compared to LAB single strains of L. bulgaricus or S. thermophilus yoghurt.
Co-Authors Annisa Sekar Salsabila Bella Khoirunisa Prihandini Darmawan Anton Budhi Dinda Victoria Hajid Rahmadianto Mardihusodo Idsap Peramiarti Nendyah Roestijawati