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All Journal Mandala of Health : A Scientific Journal Medical and Health Journal
Hajid Rahmadianto Mardihusodo
Universitas Jenderal Soedirman
Dentistry Health Professions Medicine & Pharmacology Public Health

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UJI EFEKTIVITAS YOGHURT SINGLE DAN DOUBLE STRAIN DALAM MENGHAMBAT BAKTERI Salmonella typhi PENYEBAB DEMAM TIFOID Anriani Puspita Karunia Ning Widhi; Annisa Sekar Salsabila; Hajid Rahmadianto Mardihusodo; IDSAP Peramiarti
Mandala Of Health Vol 15 No 2 (2022): Mandala Of Health
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.519 KB) | DOI: 10.20884/1.mandala.2022.15.2.6420

Abstract

Salmonella typhi adalah bakteri gram negatif penyebab demam tifoid. Penyakit ini dapat dicegah dengan mengonsumsi makanan fermentasi yang mengandung bakteri asam laktat (BAL). Yoghurt merupakan salah satu produk olahan hasil fermentasi BAL dengan bahan dasar susu. BAL yang digunakan dalam pembuatan yoghurt adalah single strain Lactobacillus bulgaricus, Streptococcus thermophilus serta double strain Lactobacillus bulgaricus dan Streptococcus thermophilus. Penelitian ini bertujuan untuk mengetahui perbandingan BAL yoghurt single strain dengan double strain dalam menghambat pertumbuhan Salmonella typhi dengan menghitung rata-rata zona hambat yang terbentuk. Metode penelitian ini adalah experimental design dengan post test only with control group design. Pengujian bakteri menggunakan difusi sumuran. Total sampel sebanyak 16 sampel dibagi menjadi 4 kelompok konsentrasi masing-masing 0%, 20%, 40%, 60%, dan 80%. Rancangan percobaan pada penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL). Analisis data menggunakan uji Kruskal Wallis dan Post Hoc Mann Whitney. Hasil penelitian didapatkan median diameter zona hambat BAL yoghurt double strain Streptococcus thermophilus dan Lactobacillus bulgaricus > single strain Lactobacillus bulgaricus > Streptococcus thermophilus. Pada uji Kruskal Wallis didapatkan nilai p = 0,000, terdapat perbedaan antara kelompok yang diberi perlakuan dan tidak diberi perlakuan. Hasil uji penelitian didapatkan perbedaan signifikan median diameter zona hambat antar kelompok. Kesimpulan dari penelitian ini menunjukan bahwa yoghurt double strain memiliki kemampuan penghambatan pertumbuhan Salmonellla typhi yang lebih baik dibandingkan yoghurt single strain Lactobacillus Blugaricus atau Streptococcus thermophilus.
DISTRIBUSI SPASIAL KUALITAS AIR TANAH DAN DESKRIPSI PENYAKIT YANG BERKAITAN DENGAN AIR DI DESA BRINGKENG, KECAMATAN KAWUNGANTEN, KABUPATEN CILACAP Siti Munfiah; Hajid Rahmadianto Mardihusodo; Tirta Wardana; Yudhi Wibowo; Joko Mulyanto
Medical and Health Journal Vol 2 No 2 (2023): February
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.431 KB) | DOI: 10.20884/1.mhj.2023.2.2.8361

Abstract

Access to clean water is fundamental for the population health, but for many rural and remote areas in Indonesia include Cilacap Regency those accesses are still limited. Furthermore, studies that assess the water quality in remote and rural areas in Indonesia are still lacking. Most of the people in Cilacap regency use dug wells and ponds as sources of clean water. Preliminary observations indicate that well and pond water smells fishy, reddish-yellow, or brownish. This condition indicates high iron levels. The test results showed that the iron content exceeded the standard in the pond in Sumbereja Hamlet, RT 03 RW 02, which was 9.8 mg/l (standard quality of 1 mg/l). This study aims to determine the spatial distribution of the water quality and the health-related problems in the rural areas of Cilacap Regency, Central Java, Indonesia. This was a descriptive observational study with a cross-sectional approach. We purposely sampled 40 wells and ponds located in Bringkeng Village, Cilacap Regency, Indonesia and assess the water quality by measuring the level. The results showed the lowest and the highest level of TDS (125.90-5090 mg/l), color (40 -2047 TCU), iron (0.16-6.45 mg/l), manganese (<0.1- 3.61 mg/l). Health problems experienced by respondents and or their families during the last three months, namely 3 people with diarrhea, 7 people with diabetes, 12 people with gout arthritis, 10 people with hypertension, 1 person with nervous system disorders, and 7 people with dry skin disorders/scaly. All dug wells and ponds did not meet the quality standards based on TDS, color, iron, and manganese levels. Water from dug wells and ponds needs special treatment to obtain water that is suitable for hygiene and sanitation purposes.
Co-Authors Annisa Sekar Salsabila Anriani Puspita Karunia Ning Widhi Idsap Peramiarti Joko Mulyanto Siti Munfiah Tirta Wardana Yudhi Wibowo