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All Journal Jurnal Pendidikan dan Pembelajaran Jurnal Teknologi Laboratorium
Eka Puspitasari, Eka
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Chemistry Education Other

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The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O Turista, Dora Dayu Rahma; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.651 KB) | DOI: 10.29238/teknolabjournal.v8i1.155

Abstract

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.
Human immune response to SARS-CoV-2 infection Wina Nurtias, Lia Yosaneri; Rahma Turista, Dora Dayu; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.717 KB) | DOI: 10.29238/teknolabjournal.v9i1.223

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COVID-19 is an acute respiratory infection caused by a new type of Coronavirus, SARS-CoV-2, which first appeared in Wuhan, China in December 2019. COVID-19 then became a pandemic in various countries in early 2020. In this article it contains review that discusses the immune response in humans due to SARS-CoV-2 infection, using the narrative literature review method, a total of 36 articles (6 from Elsevier, 24 from PMC, and 6 from Springer). It is known that the pathogenesis of COVID-19 and the manufacture of drugs and vaccines are still under investigation, but in infected patients, innate immune responses in the form of alveolar macrophages, dendritic cells, airway epithelial cells, congenital lymphocytes, and neutrophils work together in the fight against infection. Next comes the adaptive immune response in the form of antibodies (immunoglobulins) which help in fighting infections due to SARS-CoV-2. These immune responses include increasing levels of cytokines, coagulation parameters, C-reactive protein, neutrophils, and decreasing total lymphocytes. It is also known that COVID-19 patients with severe disease often experience higher total antibody, IgM responses, and IgG responses than COVID-19 patients without congenital disease. IgG antibodies are present in the serum, so the serum in COVID-19 patients who have recovered can be used for therapy in COVID-19 patients who have not healed, as long as the drug and vaccine are under investigation.
PENINGKATAN AKTIVITAS DAN HASIL BELAJAR IPS MENGGUNAKAN COURSE REVIEW HORAY DI MIN PONTIANAK TENGGARA Puspitasari, Eka; ., Sugiyono; ., Syamsiati
Jurnal Pendidikan dan Pembelajaran Khatulistiwa Vol 6, No 2 (2017): Februari 2017
Publisher : Jurnal Pendidikan dan Pembelajaran Khatulistiwa

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Abstract

Abstrak: Penelitian ini bertujuan untuk mendeskripsikan peningkatan aktivitas dan hasil belajar siswa dalam pembelajaran ilmu pengetahuan sosial menggunakan  tipe course review horay di kelas VB MIN Bangka Belitung Pontianak Tenggara. Metode penelitian menggunakan metode deskriptif. Bentuk penelitian adalah penelitian tindakan  kelas yang bersifat kolaboratif. Penelitian dilaksanakan dalam empat siklus yang terdiri dari tahap perencanaan, pelaksanaan, observasi dan refleksi. Teknik pengumpulan data adalah teknik observasi langsung, dan analisis dokumen. Aktivitas belajar siswa menggunakan tipe course review horay siklus I yaitu 71,06%, siklus II 76,56%, siklus III  81,32% dan siklus IV 87,55%, sedangkan hasil belajar menggunakan tipe course review horay baseline yaitu 67,05, siklus I 71,79, siklus II 78,46, siklus III 85,13 dan siklus IV 90,26. Dengan demikian, tipe course review horay dapat meningkatkan aktivitas dan hasil belajar siswa. Kata Kunci: Aktivitas, Hasil, Course Review Horay Absract: The purpose of this research was to describe the improvement of students’ activity and learning outcomes on social sciences learning by using the course reviews horay model to the VB students of MIN Bangka Belitung in South East Pontianak. The method of research was descriptive methods in form of collaborative classroom action research. The Research was carried out in four cycles consist of planning; implementation; observation; and reflection. The data collection technique was direct observation and document analysis. As the result, the students’ activity by using Course Review Horay at the first, second, third, and fourth cycle was 71.06%; 76.56%; 81.32%; and 87.55% respectively. While the learning outcomes by using Course Review Horay baseline was 67.05, with the result at the first, second, third, and fourth cycle was 71.79, 78.46, 85.13, and 90.26 respectively. Thus, the course review horay model can enhance the students’ activity and learning outcomes.   Key Words: Activity, Outcomes, Course Review Horay
Co-Authors Achmad Amirudin, Achmad Sugiyono . Syamsiati . Turista, Dora Dayu Rahma Wina Nurtias, Lia Yosaneri