Turista, Dora Dayu Rahma
Departemen Pendidikan Biologi, Fakultas Keguruan Dan Ilmu Pendidikan, Universitas Mulawarman, Samarinda, Kalimantan Timur

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Biodegradation of organic liquid waste by using consortium bacteria as material preparation of environmental pollution course textbook Turista, Dora Dayu Rahma
Jurnal Pendidikan Biologi Indonesia Vol 3, No 2 (2017): JULY
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (945.398 KB) | DOI: 10.22219/jpbi.v3i2.4322

Abstract

Organic waste is one waste type which oftenly pollutes the waters. Biodegradation can be used as an environmental remedy solution that is contaminated by organic matter. This research aimed to determine the ability of bacteria consortium in degrading of organic liquid waste, and construct the textbook for Environmental Pollution subject based on research of biodegradation organic waste by using bacteria consortium. This research was done through two stages. The first stage was an experimental research by using Randomized Complete Designe with bacterial type treatment and 3 repetitions, while the second phase of research was a developmental research from the first stage. The results of the first phase showed that the combination of 3 indigenous isolats bacteria (Enterobacter gergoviae, Vibrio parahaemolyticus, and Pseudomonas stutzeri) was the highest potential bacteria in decreasing BOD (71.75% ), COD (74.40%), TSS (58.44%), and increasing DO (84.15%). The second phase was Educational Research and Development of teaching materials which refers to the development model of Borg & Gall. The stages of research were: Research and Information Collecting, Planning, Develop Preliminary Form of Product, Preliminary Field Testing and Main Product Revision which was produced as textbook for the Environmental Pollution course entitled Biodegradation Organic Waste by Using Bacteria Consortium.
The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O Turista, Dora Dayu Rahma; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.651 KB) | DOI: 10.29238/teknolabjournal.v8i1.155

Abstract

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.
Human immune response to SARS-CoV-2 infection Wina Nurtias, Lia Yosaneri; Rahma Turista, Dora Dayu; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.717 KB) | DOI: 10.29238/teknolabjournal.v9i1.223

Abstract

COVID-19 is an acute respiratory infection caused by a new type of Coronavirus, SARS-CoV-2, which first appeared in Wuhan, China in December 2019. COVID-19 then became a pandemic in various countries in early 2020. In this article it contains review that discusses the immune response in humans due to SARS-CoV-2 infection, using the narrative literature review method, a total of 36 articles (6 from Elsevier, 24 from PMC, and 6 from Springer). It is known that the pathogenesis of COVID-19 and the manufacture of drugs and vaccines are still under investigation, but in infected patients, innate immune responses in the form of alveolar macrophages, dendritic cells, airway epithelial cells, congenital lymphocytes, and neutrophils work together in the fight against infection. Next comes the adaptive immune response in the form of antibodies (immunoglobulins) which help in fighting infections due to SARS-CoV-2. These immune responses include increasing levels of cytokines, coagulation parameters, C-reactive protein, neutrophils, and decreasing total lymphocytes. It is also known that COVID-19 patients with severe disease often experience higher total antibody, IgM responses, and IgG responses than COVID-19 patients without congenital disease. IgG antibodies are present in the serum, so the serum in COVID-19 patients who have recovered can be used for therapy in COVID-19 patients who have not healed, as long as the drug and vaccine are under investigation.
Concept of SARS-CoV-2 Vaccine Design to Fight COVID-19 Pandemic: A Review Insight Viol Dhea Kharisma; Arif Nur Muhammad Ansori; Rasyadan Taufiq Probojati; Dora Dayu Rahma Turista; Yulanda Antonius
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17127

Abstract

Cluster of pneumonia infection emerged in Wuhan, China due to severe acute respiratory syndromecoronavirus 2 (SARS-CoV-2). Additionally, more than 190 countries have confirmed 82 million casesof SARS-CoV-2 infection. Currently, there is a SARS-CoV-2 epidemic, and no effective prophylacticmethods are available. A vaccine is considered as an effective method to restrict an epidemic. Severalvaccine designing techniques have been established, which is enabling researchers from various institutesfor developing vaccine towards SARS-CoV-2 infections. In this review, we condense the development ofvaccine research against SARS-CoV-2.
The Potential Use of EDTA as an Alternative to Defibrination in Preparing Blood Agar Plates with Human AB Blood Type on Staphylococcus aureus Culture Dora Dayu Rahma Turista; Eka Puspitasari; Fanny Kurnanda Razvi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1923

Abstract

Blood Agar Plates (BAP) are composed of blood as one of the compositions. Sheep’s blood is usually used, but since it is difficult to be obtained, human AB blood type was used as an alternative. In preparing BAP, blood is defibrinated to lyse the blood clotting factors. Blood clots can also be prevented by adding anticoagulants, such as ethylenediaminetetraacetic acid (EDTA). This study aims to investigate the potential use of EDTA as a substitute for defibrination in preparing BAP with human AB blood type. This study employed a completely randomized design with true experimental method using Staphylococcus aureus as the sample. The parameters were the number of colonies, types of hemolysis, and hemolysis zone. The results showed that the S. aureus grown on BAP with EDTA-human AB blood type was 64 colonies (mean), produced β-hemolytic pattern, and 6 mm hemolytic zone. In contrast, the S. aureus grown on BAP with defibrinated human AB blood type showed 82 colonies (mean), β-hemolytic pattern, and 5 mm hemolytic zone. There were significant differences in the number of colonies (0.000 < α) and hemolytic zones (0.02 < α). However, there was no difference in the hemolysis type (both treatments produced β-hemolysis). EDTA was possible to be used as a substitute for defibrination in preparing BAP to assess the hemolysis type of S. aureus, but it might not be able to be used as a benchmark for counting the number of colonies and determining the hemolysis zone of S. aureus.
UJI ANTIFUNGI KETEPENG CINA (Cassia alata L.) TERHADAP Trichophyton rubrum DAN Candida albicans Qurrotu A&#039;yunin Lathifah; Eka Puspitasari; Dora Dayu Rahma Turista
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.7362

Abstract

Ketepeng Cina merupakan salah satu tumbuhan liar di Indonesia yang berpotensi sebagai antijamur. Pengujian antijamur dilakukan dengan cara difusi cakram menggunakan ekstrak dengan konsentrasi 20%, 40%, 60%, dan 80%. Hasil pengujian menunjukkan zona hambat pada semua konsentrasi ekstrak dan zona terluas dihasilkan pada konsentrasi 90%.
Implementing of Islamic Learning Integrated in Biology Education through Team Teaching Method to Enhance Students’ Understanding of Sex Education Aldeva Ilhami; Nurhayati Zein; Suci habibah; Zulfahmi Zulfahmi; Niki Dian Permana; Dora Dayu Rahma Turista
Bioeducation Journal Vol 5 No 1 (2021): Bioeducation Journal
Publisher : Universitas Negeri Padang Address: Biology Education Study Program Faculty Mathematics and Natural Science (FMIPA) Jl. Prof. Dr. Hamka Air Tawar Barat, Padang-West Sumatera-Indonesia Telp. +62751-7057420 - Fax.+62751-7058772 - Ph. +6281363229286

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sex education is needed due to increasing in teenager's sexually deviant behavior increase. The integration of Islamic values in biology learning is teachers' strategy to prevent free sex deviant behavior in the disrupssion digital era. The aim of this study was to determine the effect of the implementation of integrated Islamic biology learning with the team teaching method on students' understanding of sex education at SMA 12 Pekanbaru. The research design was a Quasi-experimental with one shot case study design. Population and sample are students at SMAN 12 Pekanbaru. Collection methods using tests and question-naires. The data analysis technique used is descriptive statistics. The results showed that the average level of understanding of students' sex education in the knowledge domain of students was 73% with a good category and an attitude domain of 79% with a good category. It can be concluded that the implementation of integrated Islamic biology learning with the team teaching method has a positive contribution to students' understanding of sex education. Biology and Islamic education teachers can collaborate in designing sex education learning as an effort to prevent adolescent sexual deviant behavior.
Identification of formalin and borax on tuna in Ngemplak market Tulungagung regency Qurrotu A’yunin Lathifah; Dora Dayu Rahma Turista; Ludfiana Azizah; Ahmad Erfan Khulaifi
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.245 KB) | DOI: 10.35584/melysa.v1i1.15

Abstract

Misuse of formalin and borax is still common, including processing and preserving fish. Therefore, this study aims to identify the presence of formalin and borax in tuna on the market Ngemplak, Tulungagung regency. The samples used in this study were fresh tuna in the Ngemplak market originating from the beaches of Prigi, Sine, Tambakrejo, Popoh and Sidem. The samples were tested qualitatively using the colorimetric method by Schryver reagent for formalin and curcumin paper for borax. The results obtained in this study, from the five tuna samples not detected contained formalin and borax which were marked by no change in color in the test. Tuna sold in the Ngemplak market Tulungagung regency negatively contains formalin and borax, making it safe for consumption. It is hoped that the relevant office will carry out routine checks on the misuse of food additives, especially preservatives circulating in the community and the public should always be careful in choosing safe food.
Identification of Salmonella sp in Islamic Boarding School in Bath Water Sumbergempol Sudistrict Hariyanto Hariyanto; Dora Dayu Rahma Turista; Nala Ulya; Emma Rizmanda
International Journal of Clinical Inventions and Medical Sciences Vol 2 No 2 (2020)
Publisher : Lamintang Education and Training Centre, in collaboration with the International Association of Educators, Scientists, Technologists, and Engineers (IA-ESTE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36079/lamintang.ijcims-0202.103

Abstract

Salmonella is a bacterium that causes typhoid fever. Salmonella sp is able to live in water, dry waste, dust and if these bacteria live in a suitable living place then the bacteria multiply reach infective dose. The bathroom water is usually used for various things one of them gargling it allows the pathogenic bacteria left in the mouth and swallowed into the digestive tract so causing abdominal pain and fever. The purpose of this study is to determine whether there are Salmonella sp bacteria in the bathwater bath in the boarding school. This research is descriptive research. Which was held on 7 to 10 May 2018 at the Microbiology Laboratory DIII Health Analyst STIKes Hutama Abdi Husada Tulungagung. Population in this research is all boarding school existing in Sumbergempol District, sampling technique in this research is total sampling, which obtained as many as 6 samples of boarding school. The instruments used are sterile tube, measuring pipette, incubator, plate, plate agar medium, aquadest, oven and laboratory equipment required. The results obtained that from 6 samples, 1 positive Salmonella sp bacteria and 5 samples of Salmonella sp bacteria. The results of the research are then analyzed descriptively. Based on the results of the study it can be concluded that 16.67% positive Salmonella sp identified and 83.33% negative or unidentified Salmonella sp. The presence of Salmonella sp in the bathroom tub is caused by a bathroom tub that has a distance with a septic tank less than 10 meters. Research on the identification of bacteria other than Salmonella sp is also needed to know the presence or absence of other bacteria that exist in the water bathtub boarding school District Sumbergempol.
The CORRELATION OF BLOOD SUGAR LEVELS WITH THE ELASTICITY OF CORONARY ARTERIES INDAH ROHMAWATI; Dora Dayu Rahma Turista
INTERNATIONAL JOURNAL OF NURSING AND MIDWIFERY SCIENCE (IJNMS) Vol 3 No 1 (2019): IJNMS Volume 3 Issue 1 April 2019
Publisher : Bina Sehat Press. Departement Research and Community Engagement Bina Sehat PPNI Institute of Health Science, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.29082/IJNMS/2019/Vol3/Iss1/209

Abstract

Coronary arteries are blood vessels that carry oxygen and nutrients to the heart muscle. These vessels can experience constriction due to the accumulation of plaque which causes atherosclerosis. Atherosclerosis is characterized by reduced coronary artery elasticity. Blood glucose levels are one of the factors that cause atherosclerosis. The aims of this study was to determine whether there was a correlation of blood sugar levels with the elasticity of coronary arteries. This study is an analytical study with cross sectional design. The population in this study were all patients who visited the Gipateda Tulungagung treatment clinic that was more than 30 years old and female. Through accidental sampling technique, 30 respondents were obtained. The study was conducted from March 1, 2016 to May 1, 2016. Data was collected using a quantum resonance magnetic analyzer (QMRA) to determine the value of blood sugar levels and the value of elasticity of coronary arteries. The data obtained were analyzed statistically by bivariate linear regression tests to determine whether there was a significant correlation of blood sugar levels with the elasticity of coronary arteries. The results showed that the p value is 0.000 < 0.05, which means that there was a significant correlation between blood sugar levels and the elasticity of the coronary arteries. High blood glucose levels can damage the vascular endothelium and also increasing platelet agegrat, so that can resulting atherosclerosis. Every individual should be able to control blood sugar levels with a healthy lifestyle and early detection regularly to prevent the decrease of coronary arteries elasticity because that can lead to coronary heart disease (CHD).