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Dinamika Fisika Kimia Air Tambak Udang Vaname (Litopenaeus vannamei) Yang Dipelihara Dengan Sistem Semi Bioflok Irsyah Rahmi; Diana Arfiati; Muhammad Musa; Karimah Karimah
Jurnal Penelitian Pendidikan IPA Vol 9 No 1 (2023): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i1.2528

Abstract

Vannamei shrimp (Litopenaeus vannamei) is one of the leading commodities in Indonesian aquaculture. The cultivation potential that is developing at this time is with intensive technology. However, along with the development of intensive technology, it experienced problems, namely crop failure caused by a decrease in water quality. This study aims to examine the physical and chemical dynamics of vannamei shrimp pond water cultivated with a semi-biofloc system. This study used a survey method and was statistically analyzed using Ms. Excel and Principal component analysis (PCA). Physical and chemical parameters of water that are measured and observed include Temperature, Brightness, pH, Dissolved Oxygen, Salinity, Carbon Dioxide (CO2), Total Organic Matter, Nitrate (NO3), Phosphate (PO4-) and Ammonia (NH3). The results showed that all the physical and chemical parameters of pond water experienced daily and weekly fluctuations during shrimp rearing. Average temperature fluctuations during the study ranged from 26.7 – 32.5 oC, brightness ranged from 26.5 – 85.0 cm, pH ranged from 7 – 8 tended to be normal, dissolved oxygen ranged from 5.8 – 8.8 mg/L, salinity ranged from 18.3 – 22.8, carbon dioxide (CO2) average values during rearing were 0 mg/L, TOM ranged from 18.42 – 71.80 mg/l, nitrate 0.022 – 0.136 mg/l, phosphate 0.017 – 0.136 mg/L and ammonia ranging from 0.008 – 0.088 mg/l. While, the results of the PCA analysis show that dissolved oxygen and brightness significantly affect the life of the shrimp that are cultivated. So it can be concluded that the dynamics of the physics-chemistry of water measured during the study showed that dissolved oxygen and brightness fluctuated and affected aquaculture activities.
Uji Aktivitas Senyawa Antibakteri Ekstrak Aloe Vera (Aloe Vera) Terhadap Infeksi Bakteri Edwardsiella Tarda secara In Vitro Nuranti Anarkhis; Arief Prajitno; Maftuch Maftuch; Karimah Karimah
Jurnal Penelitian Pendidikan IPA Vol 9 No 2 (2023): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i2.2610

Abstract

Edwardsiella tarda is a type of gram-negative intracellular pathogen that can cause mass demise in fish farming. The development of edwarsiellosis due to E.tarda infection causes significant economic losses for cultivators. Aloe vera is a perennial succulent plant like a cactus, drought withstand, and belongs to the Liliaceae family, of which there are more than 360 known species (Wijaya and Masfufatun, 2022). Aloe vera is one of the plants known to contain active ingredients that can inhibit the growth of bacteria. The results of phytochemical analysis of a crude extract of Aloe vera showed the presence of active compounds, specifically flavonoids, tannins, triterpenoids, and saponins. MIC analysis showed that the lowest absorbance value of Aloe vera was obtained at a concentration of 50 mg/L with a value of 0.316, indicating a decrease at each concentration. The concentration of 50 mg/L is close to the positive control which indicates that at a concentration of 50 mg/L, it can be the reference for the lowest concentration in determining the dose of Aloe vera extract which is known that the presence of antibacterial compounds in Aloe vera extract can inhibit the growth of E.tarda bacteria. The inhibition zone test was carried out with graded concentrations of 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L and 250 mg/L with an observation time of 24 hours post-incubation and 48 hours post-incubation. Inhibition zone analysis with the highest diameter was at 200 mg/L. SEM analysis by distributing a crude extract of Aloe vera affected E.tarda bacteria specifically, lysis occurring in bacterial cells